##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063901_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1302252
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.014430386745424	32.0	32.0	32.0	32.0	32.0
2	30.41377398537303	32.0	32.0	32.0	32.0	32.0
3	30.44675838470588	32.0	32.0	32.0	21.0	32.0
4	30.434716168606382	32.0	32.0	32.0	21.0	32.0
5	30.36412307295362	32.0	32.0	32.0	21.0	32.0
6	33.73770437672586	36.0	36.0	36.0	27.0	36.0
7	33.71551512303302	36.0	36.0	36.0	27.0	36.0
8	33.630334988926876	36.0	36.0	36.0	21.0	36.0
9	33.91600780801258	36.0	36.0	36.0	32.0	36.0
10	33.35905185785854	36.0	36.0	36.0	21.0	36.0
11	33.92383885760974	36.0	36.0	36.0	32.0	36.0
12	33.5938067286516	36.0	36.0	36.0	27.0	36.0
13	33.78617579393236	36.0	36.0	36.0	32.0	36.0
14	33.597177811974944	36.0	36.0	36.0	27.0	36.0
15	33.474302976689614	36.0	36.0	36.0	21.0	36.0
16	33.490088707869134	36.0	36.0	36.0	21.0	36.0
17	33.39286328606138	36.0	36.0	36.0	21.0	36.0
18	33.4002259163357	36.0	36.0	36.0	21.0	36.0
19	33.38033652472793	36.0	36.0	36.0	21.0	36.0
20	33.36557670865547	36.0	36.0	36.0	21.0	36.0
21	33.320579273443236	36.0	36.0	36.0	21.0	36.0
22	33.2906104194887	36.0	36.0	36.0	21.0	36.0
23	33.23596201042502	36.0	36.0	36.0	21.0	36.0
24	33.19448232753722	36.0	36.0	36.0	21.0	36.0
25	32.37930677011823	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	6.0
5	17.0
6	43.0
7	31.0
8	76.0
9	68.0
10	103.0
11	59.0
12	83.0
13	60.0
14	260.0
15	563.0
16	892.0
17	1238.0
18	1499.0
19	1929.0
20	2445.0
21	3497.0
22	4644.0
23	6518.0
24	9010.0
25	12576.0
26	17526.0
27	24286.0
28	34065.0
29	46851.0
30	65427.0
31	95338.0
32	140640.0
33	198279.0
34	335251.0
35	298970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89498520858923	17.94665566625108	11.88137065792779	25.2769884672319
2	16.294139239709228	20.176917890378178	38.454567934227526	25.07437493568507
3	18.408079014742313	24.993127216376728	28.900400694803867	27.69839307407709
4	12.090746640925989	16.22760841302423	36.914912161472074	34.766732784577705
5	14.060527310091878	37.30182865954617	34.022498072427574	14.61514595793438
6	32.84133663157232	36.44118887128497	17.6740964082116	13.04337808893111
7	29.476393386215754	30.820624758066	21.589652290922945	18.1133295647953
8	27.672217784059484	33.1193541449026	19.936397714004794	19.272030357033124
9	27.200651475807813	14.599818693053484	19.038996358496075	39.160533472642626
10	15.43933083325458	27.245557058880927	32.03197089029387	25.28314121757062
11	36.224443933961226	21.60496435538134	23.16473814131695	19.00585356934048
12	24.398718105340638	23.93548729130987	29.613656742048285	22.052137861301212
13	29.014630368252536	20.320698655598164	25.742102590069237	24.92256838608006
14	23.2722983771822	20.142995451192526	26.09417260880256	30.49053356282272
15	24.63141428273976	27.92492522256515	22.936023707500947	24.50763678719414
16	25.084751492947877	26.24714549595375	24.585232494168054	24.08287051693032
17	23.339408402958448	26.364393535429283	26.068195473817013	24.22800258779526
18	24.072921373527233	25.57596603990496	26.990867537800895	23.360245048766917
19	24.954853880260963	25.516048965289357	26.008545103855283	23.520552050594393
20	25.181281372666547	24.884345711800275	25.69346464897963	24.24090826655355
21	26.14234086223365	24.837071912742317	24.968782202358952	24.051805022665082
22	25.199956965449406	24.92599594245666	25.518873724332963	24.355173367760973
23	23.686807207805384	25.053717331562737	25.944395261193797	25.315080199438082
24	24.117850921243427	25.676937369893626	25.746942188007893	24.45826952085506
25	24.19969491694748	25.23866793208252	25.714658971708733	24.84697817926127
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	100.0
2	320.5
3	541.0
4	541.0
5	541.0
6	1375.5
7	2210.0
8	2210.0
9	2210.0
10	2375.5
11	2541.0
12	2541.0
13	2541.0
14	3114.0
15	3687.0
16	3687.0
17	3687.0
18	6519.5
19	9352.0
20	9352.0
21	9352.0
22	15476.0
23	21600.0
24	21600.0
25	21600.0
26	32774.0
27	43948.0
28	43948.0
29	43948.0
30	55285.5
31	66623.0
32	66623.0
33	66623.0
34	79992.0
35	93361.0
36	93361.0
37	93361.0
38	105837.5
39	118314.0
40	118314.0
41	118314.0
42	132431.5
43	146549.0
44	146549.0
45	146549.0
46	160554.5
47	174560.0
48	174560.0
49	174560.0
50	173744.5
51	172929.0
52	172929.0
53	172929.0
54	157461.0
55	141993.0
56	141993.0
57	141993.0
58	127505.5
59	113018.0
60	113018.0
61	113018.0
62	97988.5
63	82959.0
64	82959.0
65	82959.0
66	67761.5
67	52564.0
68	52564.0
69	52564.0
70	39624.0
71	26684.0
72	26684.0
73	26684.0
74	20279.0
75	13874.0
76	13874.0
77	13874.0
78	11308.0
79	8742.0
80	8742.0
81	8742.0
82	5944.0
83	3146.0
84	3146.0
85	3146.0
86	2392.5
87	1639.0
88	1639.0
89	1639.0
90	1114.0
91	589.0
92	589.0
93	589.0
94	372.5
95	156.0
96	156.0
97	156.0
98	364.5
99	573.0
100	573.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03708959556214926
2	0.005068143492964495
3	0.0010750607409318626
4	0.0025340717464822477
5	0.0073718450806756295
6	0.01098097756808974
7	0.01873677291338389
8	0.031637501804566245
9	0.04545971133083305
10	0.053215506676127206
11	0.050143904559179026
12	0.056901429216465016
13	0.0599730313334132
14	0.06235352297404804
15	0.055519208263838334
16	0.06143204233896358
17	0.05820686011616799
18	0.07371845080675629
19	0.07080042879565553
20	0.07371845080675629
21	0.06995573821349478
22	0.07463993144184075
23	0.07648289271200966
24	0.07010931831934218
25	0.07425598117722224
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1302252.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.87016326525125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63891389693124	40.20810477568716
2	14.723980911531282	15.86366511237266
3	5.057494710877759	8.173441973643886
4	2.192594222496197	4.7246163496126705
5	1.0784953455399153	2.9049360172524405
6	0.607749887290297	1.9643751391659807
7	0.3701429176545348	1.3957761583868367
8	0.2454132386411019	1.057636098644018
9	0.17961769095642413	0.870843090343501
>10	0.751642570033492	7.106902817235174
>50	0.06853977044934378	2.617516788955377
>100	0.07543315557852324	8.560275924717898
>500	0.007842750158399917	3.0045200770798157
>1k	0.002138931861381796	1.547389676902713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2837	0.21785338014454958	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2069	0.15887861949914456	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1496	0.11487791917386189	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3037015877111342E-4
2	0.0	0.0	0.0	0.0	2.3037015877111342E-4
3	0.0	0.0	0.0	0.0	2.3037015877111342E-4
4	0.0	0.0	0.0	0.0	2.3037015877111342E-4
5	0.0	0.0	0.0	0.0	2.3037015877111342E-4
6	0.0	0.0	0.0	0.0	3.071602116948179E-4
7	0.0	0.0	0.0	0.0	3.071602116948179E-4
8	0.0	0.0	0.0	0.0	3.071602116948179E-4
9	0.0	0.0	0.0	0.0	3.071602116948179E-4
10	0.0	0.0	0.0	0.0	3.839502646185224E-4
11	0.0	0.0	0.0	0.0	3.839502646185224E-4
12	0.0	0.0	0.0	7.679005292370447E-5	9.214806350844537E-4
13	0.0	0.0	0.0	7.679005292370447E-5	0.0012286408467792715
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGT	80	2.0009593E-6	13.061179	6
GTCTTAG	110	2.752131E-9	12.95224	1
GTATTAG	155	0.0	12.868677	1
GCTCGTA	120	7.385097E-10	12.667333	9
GTCCTAA	155	1.8189894E-12	12.255883	1
CGTCTTA	70	1.0885381E-4	12.216806	15
ACGCGCG	55	0.0030632468	12.093404	13
GGCTAAG	55	0.0030720772	12.0887575	1
AAGCTCG	120	1.0002623E-8	11.875168	7
CGAACGA	145	9.640644E-11	11.795537	16
CGTCAAT	145	9.640644E-11	11.79463	19
CCGATAA	145	9.640644E-11	11.793723	9
CGATAAC	145	9.640644E-11	11.793723	10
ATTAACC	145	9.822543E-11	11.787834	3
CGAGCCG	210	0.0	11.764333	15
CGGTCCA	210	0.0	11.762523	10
GTGCGTA	65	8.0183404E-4	11.692923	10
ATTAGCC	65	8.052013E-4	11.687083	3
TCGCCAG	155	2.5465852E-11	11.647117	17
ACGAACG	150	1.7462298E-10	11.402352	15
>>END_MODULE