FastQCFastQC Report
Thu 2 Feb 2017
SRR4063901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302252
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG3000.021.9983314
GATTTCG1105.2586984E-921.9983341
GTTATAT1553.45608E-1119.872511
GTATCAA31700.019.0866551
CGCGGGA2900.018.20621544
AGGACGT3500.017.5986655
GGACGTG3650.017.4781256
ATTTCGT1551.4406396E-817.03096442
TTAGGAC3500.016.9707933
AATCACG1954.9112714E-1116.92374234
TATGGCG3650.016.87672816
GCATTCG4250.016.56408322
GACGTGA2151.2732926E-1116.370857
AATAGCG2600.016.0757035
GCCGCTA4400.016.0006335
CGTATTG4450.015.82145427
ATACCGT3800.015.6303936
CGCTTCG3100.015.61471632
CGTGAAA2403.6379788E-1215.5833469
CTATTAG1704.4863555E-815.5306181