##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1302252
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97469767756164	32.0	32.0	32.0	32.0	32.0
2	30.713617640825277	32.0	32.0	32.0	32.0	32.0
3	31.11967192217789	32.0	32.0	32.0	32.0	32.0
4	31.32784130874823	32.0	32.0	32.0	32.0	32.0
5	31.158667446853606	32.0	32.0	32.0	32.0	32.0
6	34.75628065842863	36.0	36.0	36.0	32.0	36.0
7	34.802442230843184	36.0	36.0	36.0	32.0	36.0
8	34.70319799854406	36.0	36.0	36.0	32.0	36.0
9	34.93502409671861	36.0	36.0	36.0	32.0	36.0
10	34.6383726037664	36.0	36.0	36.0	32.0	36.0
11	34.948759533485074	36.0	36.0	36.0	36.0	36.0
12	34.78726928428599	36.0	36.0	36.0	32.0	36.0
13	34.878917444549906	36.0	36.0	36.0	32.0	36.0
14	34.79636199445269	36.0	36.0	36.0	32.0	36.0
15	34.73197660667828	36.0	36.0	36.0	32.0	36.0
16	34.767510435768195	36.0	36.0	36.0	32.0	36.0
17	34.71435943273652	36.0	36.0	36.0	32.0	36.0
18	34.7187871471881	36.0	36.0	36.0	32.0	36.0
19	34.71793324179959	36.0	36.0	36.0	32.0	36.0
20	34.71932621335963	36.0	36.0	36.0	32.0	36.0
21	34.71242278760179	36.0	36.0	36.0	32.0	36.0
22	34.69250882317708	36.0	36.0	36.0	32.0	36.0
23	34.62392916271198	36.0	36.0	36.0	32.0	36.0
24	34.60416109938783	36.0	36.0	36.0	32.0	36.0
25	34.57399105549464	36.0	36.0	36.0	32.0	36.0
26	34.4899957919051	36.0	36.0	36.0	32.0	36.0
27	34.48842927482546	36.0	36.0	36.0	32.0	36.0
28	34.45581730724929	36.0	36.0	36.0	32.0	36.0
29	34.42785190577553	36.0	36.0	36.0	32.0	36.0
30	34.402634820295916	36.0	36.0	36.0	32.0	36.0
31	34.403273713536244	36.0	36.0	36.0	32.0	36.0
32	34.35310677196119	36.0	36.0	36.0	32.0	36.0
33	34.31713216796749	36.0	36.0	36.0	32.0	36.0
34	34.30210665831191	36.0	36.0	36.0	32.0	36.0
35	34.25647263356094	36.0	36.0	36.0	32.0	36.0
36	34.22655830054398	36.0	36.0	36.0	32.0	36.0
37	34.21118032454548	36.0	36.0	36.0	32.0	36.0
38	34.149345134428664	36.0	36.0	36.0	32.0	36.0
39	34.152977303931955	36.0	36.0	36.0	32.0	36.0
40	34.12133673052527	36.0	36.0	36.0	32.0	36.0
41	34.09184167119728	36.0	36.0	36.0	32.0	36.0
42	34.01951772775162	36.0	36.0	36.0	32.0	36.0
43	34.01748279134914	36.0	36.0	36.0	32.0	36.0
44	33.9473105051864	36.0	36.0	36.0	32.0	36.0
45	33.89338699422232	36.0	36.0	36.0	32.0	36.0
46	33.879770582037885	36.0	36.0	36.0	32.0	36.0
47	33.86585622444811	36.0	36.0	36.0	32.0	36.0
48	33.78856319667776	36.0	36.0	36.0	32.0	36.0
49	33.76976115221939	36.0	36.0	36.0	32.0	36.0
50	33.0288016451501	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	0.0
19	1.0
20	7.0
21	27.0
22	121.0
23	388.0
24	895.0
25	2317.0
26	4894.0
27	9246.0
28	16476.0
29	26671.0
30	41290.0
31	63693.0
32	98353.0
33	165490.0
34	313551.0
35	558829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22127336705183	19.23873827519633	12.789961863189761	25.75002649456209
2	15.50549511306602	21.309381930323564	37.38473777652183	25.800385180088586
3	17.7731532852881	25.70637629631905	29.108040345710677	27.412430072682177
4	11.97510159324002	17.1909123579768	37.160549571050765	33.67343647773242
5	14.27711379978683	37.33808817341037	33.331106421798545	15.053691605004255
6	33.157151084661166	37.03520829333845	17.018160875407947	12.789479746592436
7	29.499282780905695	31.057122584568887	21.3351947242162	18.108399910309217
8	27.705697514766726	33.42148831408975	19.498376658281195	19.374437512862333
9	27.224719196033302	14.287963152806352	18.602211722389754	39.88510592877059
10	15.221973677845021	27.4106564873538	32.07451109580426	25.29285873899692
11	36.73735958938823	21.376738142847927	22.92505597994858	18.96084628781526
12	24.49671799313804	23.875486464985272	29.50166327254633	22.12613226933036
13	29.289966273705165	20.083808921188588	25.642197185175174	24.984027619931073
14	23.36114165658154	19.978683015831088	25.84730404654106	30.812871281046313
15	24.771249509477546	27.86735812093999	22.750949751302237	24.610442618280224
16	25.29180489003563	26.20807224474751	24.313183437768767	24.18693942744809
17	23.529009746193516	26.3945841511474	25.759837573680056	24.31656852897903
18	24.35590373898734	25.431349256019004	26.80750485121532	23.405242153778342
19	25.157976457687496	25.49831023358784	25.859684500146287	23.484028808578376
20	25.435191435085464	24.669128572777893	25.637611413538025	24.258068578598618
21	26.311343733778102	24.76824762027626	24.890574174583723	24.02983447136192
22	25.29441396723205	24.885659259987346	25.50044231206345	24.31948446071716
23	23.949468840756662	24.79354899151159	25.903247159138903	25.35373500859285
24	24.400209791230083	25.496315215162713	25.63791661643298	24.465558377174222
25	24.486572243877415	25.060588468378807	25.715926857662406	24.736912430081368
26	24.199773612841096	25.690411720527052	26.07760441224579	24.03221025438606
27	24.924089651772317	25.321726255842066	25.37732473041651	24.376859361969107
28	24.002580228995324	25.19163575767349	26.12981009207424	24.67597392125694
29	24.222639967809016	25.237132053680305	25.992452934509163	24.54777504400151
30	24.198920144277693	25.43489495941902	26.191377587810273	24.17480730849302
31	24.68112622018322	25.27206697174927	25.322060789718016	24.724746018349496
32	24.347824751443497	25.343970561019308	25.24444813046039	25.063756557076804
33	24.00770689235462	25.104072995850903	25.889813555560504	24.998406556233974
34	24.5258880192655	25.286356264360048	26.016798953175492	24.17095676319896
35	25.222985794182673	24.966729713188244	25.88777719159615	23.922507301032933
36	24.199760241018446	25.622033251391365	25.581254257411445	24.596952250178745
37	25.086813954738485	25.496425307746062	25.24331713010958	24.173443607405872
38	24.345239740860542	25.279613195346794	25.685094566242444	24.690052497550216
39	24.80575329631933	25.183726127122352	25.359504227428754	24.651016349129556
40	25.09447277625176	25.518515589063966	25.444258182329193	23.94275345235508
41	24.028406939857184	25.784335483256633	25.968252603060783	24.219004973825406
42	25.432697734744135	25.792924022278584	25.399908004456943	23.37447023852033
43	24.659239430087066	24.981762110342686	25.590638855996694	24.76835960357355
44	24.376631324971836	25.563510231224836	25.344502813999583	24.715355629803746
45	24.60164731792499	25.725405033488503	25.171742799703278	24.50120484888323
46	24.222784499076592	25.523461407031707	25.43085210540259	24.82290198848911
47	24.617768706103874	25.283093051722368	25.710823500140528	24.38831474203323
48	25.172223932964172	25.89997611787174	24.75348037864308	24.174319570521007
49	24.486366983734985	25.88503067399863	25.19414579306627	24.434456549200114
50	24.662734979727237	26.031760658557562	24.782605356923455	24.522899004791743
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	18.0
2	17.0
3	51.0
4	85.0
5	121.5
6	158.0
7	163.5
8	169.0
9	226.0
10	283.0
11	428.5
12	574.0
13	1089.0
14	1604.0
15	2259.0
16	2914.0
17	3281.0
18	3648.0
19	3840.5
20	4033.0
21	4580.0
22	5127.0
23	6057.0
24	6987.0
25	8032.5
26	9078.0
27	11384.5
28	13691.0
29	16623.0
30	19555.0
31	22454.0
32	25353.0
33	29282.0
34	33211.0
35	38597.0
36	43983.0
37	49888.5
38	55794.0
39	59976.0
40	64158.0
41	69370.0
42	74582.0
43	75833.5
44	77085.0
45	83093.5
46	89102.0
47	94176.5
48	99251.0
49	100907.0
50	102563.0
51	96948.0
52	91333.0
53	88631.0
54	85929.0
55	85424.5
56	84920.0
57	81263.5
58	77607.0
59	70572.0
60	63537.0
61	56174.5
62	48812.0
63	42354.0
64	35896.0
65	30532.5
66	25169.0
67	21557.5
68	17946.0
69	16360.0
70	14774.0
71	11397.0
72	8020.0
73	6802.0
74	5584.0
75	4434.5
76	3285.0
77	2854.5
78	2424.0
79	1996.0
80	1568.0
81	1263.0
82	958.0
83	819.0
84	680.0
85	516.0
86	352.0
87	249.0
88	146.0
89	128.5
90	111.0
91	83.0
92	55.0
93	48.5
94	42.0
95	40.0
96	38.0
97	30.0
98	22.0
99	21.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007525425186523038
2	3.071602116948179E-4
3	5.375303704659313E-4
4	0.0
5	0.0
6	1.5358010584740894E-4
7	0.0
8	0.0
9	6.911104763133403E-4
10	2.3037015877111342E-4
11	0.0
12	0.0
13	3.071602116948179E-4
14	3.071602116948179E-4
15	0.005298513651735609
16	9.214806350844537E-4
17	0.0
18	7.679005292370447E-5
19	7.679005292370447E-5
20	6.911104763133403E-4
21	0.0
22	3.071602116948179E-4
23	4.6074031754222684E-4
24	3.839502646185224E-4
25	0.0018429612701689074
26	0.00445382306957486
27	0.00445382306957486
28	0.003225182222795588
29	0.0018429612701689074
30	0.0031483921698718838
31	0.006987894816057108
32	0.0025340717464822477
33	0.0031483921698718838
34	0.002150121481863725
35	0.0029948120640244744
36	0.008523695874531197
37	0.003225182222795588
38	0.0073718450806756295
39	0.003225182222795588
40	0.0019197513230926118
41	0.0026876518523296566
42	0.001151850793855567
43	0.0013822209526266806
44	9.982706880081581E-4
45	0.0010750607409318626
46	5.375303704659313E-4
47	0.002303701587711134
48	0.001766171217245203
49	6.911104763133403E-4
50	9.214806350844537E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1302252.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.31954052259762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43322480542366	44.57550551312719
2	14.083080652747851	16.426375856218776
3	4.6211361925461665	8.085076183249159
4	1.9602524222175615	4.5728408228814885
5	0.9551904021005612	2.785313268105
6	0.5258462647945484	1.840026752900536
7	0.3186542745667672	1.300863961480687
8	0.20927131820956804	0.9763685698032262
9	0.14954425391200607	0.784921695834858
>10	0.6239172519610028	6.341297820216588
>50	0.055554590952960684	2.3111464549320138
>100	0.056783007196246925	6.749700081968131
>500	0.0064856772839114825	2.5648648169482757
>1k	0.0010588860871692217	0.6856982023339915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	7.679005292370447E-5
11	0.0	0.0	0.0	0.0	7.679005292370447E-5
12	0.0	0.0	0.0	7.679005292370447E-5	3.071602116948179E-4
13	0.0	0.0	0.0	7.679005292370447E-5	5.375303704659313E-4
14	0.0	0.0	0.0	7.679005292370447E-5	5.375303704659313E-4
15	0.0	0.0	0.0	7.679005292370447E-5	5.375303704659313E-4
16	0.0	0.0	0.0	7.679005292370447E-5	5.375303704659313E-4
17	0.0	0.0	0.0	7.679005292370447E-5	5.375303704659313E-4
18	0.0	0.0	0.0	7.679005292370447E-5	5.375303704659313E-4
19	0.0	0.0	0.0	7.679005292370447E-5	5.375303704659313E-4
20	0.0	0.0	0.0	7.679005292370447E-5	9.214806350844537E-4
21	0.0	0.0	0.0	1.5358010584740894E-4	9.214806350844537E-4
22	0.0	0.0	0.0	1.5358010584740894E-4	0.0010750607409318626
23	0.0	0.0	0.0	4.6074031754222684E-4	0.0010750607409318626
24	0.0	0.0	0.0	9.214806350844537E-4	0.0010750607409318626
25	0.0	0.0	0.0	0.0010750607409318626	0.0010750607409318626
26	0.0	0.0	0.0	0.001151850793855567	0.0015358010584740896
27	0.0	0.0	0.0	0.001305430899702976	0.0015358010584740896
28	0.0	0.0	0.0	0.0019965413760163163	0.0015358010584740896
29	0.0	0.0	0.0	0.0027644419052533613	0.0015358010584740896
30	0.0	0.0	0.0	0.004684193228345973	0.0015358010584740896
31	0.0	0.0	0.0	0.009061226244997129	0.0016893811643214985
32	0.0	0.0	0.0	0.014666900108427555	0.001766171217245203
33	0.0	0.0	0.0	0.020042203813086867	0.0018429612701689074
34	0.0	0.0	0.0	0.027030098629143975	0.0019197513230926118
35	0.0	0.0	0.0	0.034709103921514425	0.0019197513230926118
36	0.0	0.0	0.0	0.04676514223053602	0.0019197513230926118
37	0.0	0.0	0.0	0.06488759472053028	0.0019197513230926118
38	0.0	0.0	0.0	0.09053547239704758	0.0019197513230926118
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	300	0.0	21.998331	4
GATTTCG	110	5.2586984E-9	21.99833	41
GTTATAT	155	3.45608E-11	19.87251	1
GTATCAA	3170	0.0	19.086655	1
CGCGGGA	290	0.0	18.206215	44
AGGACGT	350	0.0	17.598665	5
GGACGTG	365	0.0	17.478125	6
ATTTCGT	155	1.4406396E-8	17.030964	42
TTAGGAC	350	0.0	16.970793	3
AATCACG	195	4.9112714E-11	16.923742	34
TATGGCG	365	0.0	16.876728	16
GCATTCG	425	0.0	16.564083	22
GACGTGA	215	1.2732926E-11	16.37085	7
AATAGCG	260	0.0	16.075703	5
GCCGCTA	440	0.0	16.00063	35
CGTATTG	445	0.0	15.821454	27
ATACCGT	380	0.0	15.630393	6
CGCTTCG	310	0.0	15.614716	32
CGTGAAA	240	3.6379788E-12	15.583346	9
CTATTAG	170	4.4863555E-8	15.530618	1
>>END_MODULE