FastQCFastQC Report
Thu 2 Feb 2017
SRR4063900_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063900_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263628
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36510.16128975255651545No Hit
TATCAACGCAGAGTACTTTTTTTTT22680.10019314127586335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.016.022091
GTATCAA25000.013.8697411
TAGGACC4050.013.1335264
GGACCGT1302.6175258E-911.6920916
CGTTATT803.7827858E-410.684232
TAGCCTA1201.526907E-610.2903385
ACCGTCG1303.838377E-710.231718
TGGACCG1409.711766E-810.1772575
GCGTTAT750.002648778710.1331441
GTGTAGG3300.010.075571
TCGCTCA1803.929017E-1010.02879
CGTCGTA951.6430492E-410.00091910
CCGGTCC951.6430492E-410.0009199
AACCGAG951.6437825E-410.0004787
GTAGGGT1901.0004442E-109.9971613
AGCCTAT1054.107294E-59.9521966
CTTAGAC1252.5802783E-69.8771953
TACCGTC1356.441169E-79.8523227
ACCGTGC1451.6156264E-79.8284898
GTCCTAC6000.09.81648351
Produced by FastQC (version 0.11.5)