##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063900_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2263628
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27575158109018	32.0	32.0	32.0	32.0	32.0
2	30.91884134672305	32.0	32.0	32.0	32.0	32.0
3	30.92728266305241	32.0	32.0	32.0	32.0	32.0
4	30.951907733956286	32.0	32.0	32.0	32.0	32.0
5	30.87877911034852	32.0	32.0	32.0	32.0	32.0
6	34.52782259275818	36.0	36.0	36.0	32.0	36.0
7	34.480073580994755	36.0	36.0	36.0	32.0	36.0
8	34.45424645745679	36.0	36.0	36.0	32.0	36.0
9	34.574180033114985	36.0	36.0	36.0	32.0	36.0
10	34.31703309907812	36.0	36.0	36.0	32.0	36.0
11	34.54581715723608	36.0	36.0	36.0	32.0	36.0
12	34.39817363983835	36.0	36.0	36.0	32.0	36.0
13	34.446768196894546	36.0	36.0	36.0	32.0	36.0
14	34.36709653706351	36.0	36.0	36.0	32.0	36.0
15	34.31893314625901	36.0	36.0	36.0	32.0	36.0
16	34.323357901563334	36.0	36.0	36.0	32.0	36.0
17	34.27095618184613	36.0	36.0	36.0	32.0	36.0
18	34.25555214902802	36.0	36.0	36.0	32.0	36.0
19	34.26196707232814	36.0	36.0	36.0	32.0	36.0
20	34.24456889559592	36.0	36.0	36.0	32.0	36.0
21	34.24198675754143	36.0	36.0	36.0	32.0	36.0
22	34.21884382062777	36.0	36.0	36.0	32.0	36.0
23	34.170655249007346	36.0	36.0	36.0	32.0	36.0
24	34.16324413728758	36.0	36.0	36.0	32.0	36.0
25	33.764101698689004	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	16.0
5	33.0
6	96.0
7	17.0
8	127.0
9	135.0
10	219.0
11	56.0
12	149.0
13	116.0
14	202.0
15	349.0
16	607.0
17	771.0
18	1012.0
19	1346.0
20	2035.0
21	2964.0
22	4511.0
23	7077.0
24	10695.0
25	15941.0
26	22857.0
27	30977.0
28	42650.0
29	57853.0
30	76065.0
31	104867.0
32	146903.0
33	208384.0
34	457456.0
35	1067134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10750066953691	17.478316284817026	12.557904999120542	25.85627804652552
2	16.01397656023581	19.65010885777096	38.304593232439615	26.03132134955361
3	18.728587692222057	23.55114192348183	28.688814641147946	29.03145574314817
4	13.056399485939998	16.284957825079115	35.635278935865756	35.02336375311513
5	14.736403551790165	36.44804194710487	33.82648043537617	14.989074065728794
6	33.5594604231201	35.316429913636064	17.430431015552756	13.69367864769108
7	30.18592130821861	30.61042601590379	20.706744883584875	18.496907792292724
8	27.800394551233325	33.54595850141239	19.29893796424291	19.35470898311137
9	27.2002376114743	15.329343381729284	18.740190681440183	38.73022832535623
10	15.943088561221613	27.60964177513192	31.84365443999572	24.603615223650745
11	35.46973658329595	22.038335650209845	22.982118428118998	19.509809338375213
12	24.683120724881512	23.8615376590585	29.089415785746553	22.365925830313437
13	29.34996238085429	20.615999144175486	25.165440250098797	24.868598224871427
14	23.360567686400298	19.842455706968853	25.794551420055928	31.002425186574918
15	24.611738849454483	27.720391854892394	22.648707092799874	25.019162202853245
16	24.87363500608727	26.54606475935172	24.109919198902965	24.470381035658047
17	23.817461759384383	26.50477037948642	25.404022387165703	24.273745473963494
18	23.966827785399218	26.18205193614375	26.273037672427474	23.57808260602955
19	25.076195587685273	25.4563556448397	25.724267023646053	23.743181743828977
20	25.533909114421572	25.244412219174084	25.48726598300952	23.734412683394822
21	25.55071999886823	24.887486361313346	25.19147212933277	24.370321510485653
22	25.3294639446543	25.204212823451183	25.2922378789808	24.174085352913714
23	24.702866505908183	25.264725362082395	25.48331445438058	24.54909367762884
24	24.458723359480725	25.39708504408421	25.614555061825765	24.5296365346093
25	24.501759173210697	25.35221043938214	25.663591613620305	24.48243877378686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	58.0
2	204.0
3	350.0
4	350.0
5	350.0
6	907.0
7	1464.0
8	1464.0
9	1464.0
10	1969.0
11	2474.0
12	2474.0
13	2474.0
14	3751.0
15	5028.0
16	5028.0
17	5028.0
18	9685.0
19	14342.0
20	14342.0
21	14342.0
22	22824.0
23	31306.0
24	31306.0
25	31306.0
26	46920.5
27	62535.0
28	62535.0
29	62535.0
30	82856.5
31	103178.0
32	103178.0
33	103178.0
34	127690.5
35	152203.0
36	152203.0
37	152203.0
38	179988.5
39	207774.0
40	207774.0
41	207774.0
42	233521.0
43	259268.0
44	259268.0
45	259268.0
46	281742.0
47	304216.0
48	304216.0
49	304216.0
50	310210.0
51	316204.0
52	316204.0
53	316204.0
54	297108.5
55	278013.0
56	278013.0
57	278013.0
58	247365.0
59	216717.0
60	216717.0
61	216717.0
62	182446.0
63	148175.0
64	148175.0
65	148175.0
66	116628.5
67	85082.0
68	85082.0
69	85082.0
70	63524.0
71	41966.0
72	41966.0
73	41966.0
74	30499.5
75	19033.0
76	19033.0
77	19033.0
78	13636.5
79	8240.0
80	8240.0
81	8240.0
82	5721.5
83	3203.0
84	3203.0
85	3203.0
86	2237.0
87	1271.0
88	1271.0
89	1271.0
90	852.0
91	433.0
92	433.0
93	433.0
94	320.0
95	207.0
96	207.0
97	207.0
98	547.5
99	888.0
100	888.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03843387694444494
2	0.00547793188633468
3	0.0015461904517880148
4	0.0034899727340358044
5	0.009100435230523743
6	0.013208884145274753
7	0.02297197242656479
8	0.03551820352107325
9	0.0490805026267567
10	0.05769499228671849
11	0.05491184947350006
12	0.06228938677203145
13	0.06547012141570965
14	0.06776731865836612
15	0.060168897009579315
16	0.06759061117816179
17	0.06357051600351295
18	0.07664686953863444
19	0.07448220290613122
20	0.07832559060057571
21	0.0742171416858247
22	0.07828141373052462
23	0.08172720959450934
24	0.07540991717720404
25	0.07819305999042245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2263628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.301265385610215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.98250945426579	23.77069766625629
2	16.718912291105255	13.47586642405147
3	8.242638204149195	9.96566249228959
4	4.758444215107829	7.670852925427293
5	2.9906081913264515	6.026264719151355
6	2.029637299304905	4.90781708614921
7	1.3741870196968562	3.8767033039184633
8	1.0028182037247515	3.2331874049465714
9	0.7499140755197661	2.720023755653397
>10	3.0462396332485655	19.571622980773746
>50	0.0775911768712412	2.1038131587853828
>100	0.02418348101231178	1.7602127527434432
>500	0.001875468063805916	0.5481154702359722
>1k	4.4128660324841074E-4	0.3691598596178599
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3651	0.16128975255651545	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2268	0.10019314127586335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	670	0.0	16.02209	1
GTATCAA	2500	0.0	13.869741	1
TAGGACC	405	0.0	13.133526	4
GGACCGT	130	2.6175258E-9	11.692091	6
CGTTATT	80	3.7827858E-4	10.68423	2
TAGCCTA	120	1.526907E-6	10.290338	5
ACCGTCG	130	3.838377E-7	10.23171	8
TGGACCG	140	9.711766E-8	10.177257	5
GCGTTAT	75	0.0026487787	10.133144	1
GTGTAGG	330	0.0	10.07557	1
TCGCTCA	180	3.929017E-10	10.0287	9
CGTCGTA	95	1.6430492E-4	10.000919	10
CCGGTCC	95	1.6430492E-4	10.000919	9
AACCGAG	95	1.6437825E-4	10.000478	7
GTAGGGT	190	1.0004442E-10	9.997161	3
AGCCTAT	105	4.107294E-5	9.952196	6
CTTAGAC	125	2.5802783E-6	9.877195	3
TACCGTC	135	6.441169E-7	9.852322	7
ACCGTGC	145	1.6156264E-7	9.828489	8
GTCCTAC	600	0.0	9.8164835	1
>>END_MODULE