FastQCFastQC Report
Thu 2 Feb 2017
SRR4063900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263628
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26850.029.8271241
GGTATCA9300.027.2062821
TATCAAC39450.019.9066772
TAGGACG5100.019.8415134
TCAACGC40500.019.3366894
ATCAACG41150.019.0308283
CAACGCA42050.018.6762375
AACGCAG43500.018.1042656
GTATAGG3250.016.9243091
GTAGGAC7800.016.6392963
TTAGGAC5550.016.2505633
TATACCG953.3500153E-416.20925
GTCCTAC7650.015.242941
ACGCAGA53100.014.8311797
AGAGTAC54300.014.42271111
CGCAGAG55100.014.4126148
GTATAGA4000.014.3010421
TAGGACC6800.014.234134
CAGAGTA56200.014.13114110
CTAGTAT1901.7571074E-713.8932923
Produced by FastQC (version 0.11.5)