##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063900_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2263628
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.385673794457393	32.0	32.0	32.0	32.0	32.0
2	31.42980958001933	32.0	32.0	32.0	32.0	32.0
3	31.488065176787	32.0	32.0	32.0	32.0	32.0
4	31.56378565736066	32.0	32.0	32.0	32.0	32.0
5	31.52099549926048	32.0	32.0	32.0	32.0	32.0
6	35.105063641198996	36.0	36.0	36.0	36.0	36.0
7	35.125058976121515	36.0	36.0	36.0	36.0	36.0
8	35.07379613611424	36.0	36.0	36.0	36.0	36.0
9	35.17658731911781	36.0	36.0	36.0	36.0	36.0
10	35.04416273345267	36.0	36.0	36.0	36.0	36.0
11	35.176172940076725	36.0	36.0	36.0	36.0	36.0
12	35.1043740402575	36.0	36.0	36.0	36.0	36.0
13	35.128218064098874	36.0	36.0	36.0	36.0	36.0
14	35.078872500251805	36.0	36.0	36.0	36.0	36.0
15	35.05483409818221	36.0	36.0	36.0	36.0	36.0
16	35.07545100166635	36.0	36.0	36.0	36.0	36.0
17	35.04895989977152	36.0	36.0	36.0	36.0	36.0
18	35.03930990427756	36.0	36.0	36.0	36.0	36.0
19	35.03793953776857	36.0	36.0	36.0	36.0	36.0
20	35.03114866930432	36.0	36.0	36.0	36.0	36.0
21	35.031774655552944	36.0	36.0	36.0	36.0	36.0
22	35.010128872765314	36.0	36.0	36.0	36.0	36.0
23	34.969011692733964	36.0	36.0	36.0	36.0	36.0
24	34.948594026933755	36.0	36.0	36.0	36.0	36.0
25	34.92577358117146	36.0	36.0	36.0	36.0	36.0
26	34.87508945816185	36.0	36.0	36.0	32.0	36.0
27	34.858459517199826	36.0	36.0	36.0	32.0	36.0
28	34.84908120945668	36.0	36.0	36.0	32.0	36.0
29	34.831288975043606	36.0	36.0	36.0	32.0	36.0
30	34.813104450024476	36.0	36.0	36.0	32.0	36.0
31	34.80708270086781	36.0	36.0	36.0	32.0	36.0
32	34.769117982283305	36.0	36.0	36.0	32.0	36.0
33	34.76375800264001	36.0	36.0	36.0	32.0	36.0
34	34.75514439651745	36.0	36.0	36.0	32.0	36.0
35	34.73507705329674	36.0	36.0	36.0	32.0	36.0
36	34.711519295573304	36.0	36.0	36.0	32.0	36.0
37	34.69276974838622	36.0	36.0	36.0	32.0	36.0
38	34.675979887154604	36.0	36.0	36.0	32.0	36.0
39	34.66044553256984	36.0	36.0	36.0	32.0	36.0
40	34.6636982755117	36.0	36.0	36.0	32.0	36.0
41	34.64124052185253	36.0	36.0	36.0	32.0	36.0
42	34.610026029011834	36.0	36.0	36.0	32.0	36.0
43	34.592255883033786	36.0	36.0	36.0	32.0	36.0
44	34.552652202570385	36.0	36.0	36.0	32.0	36.0
45	34.52956360320689	36.0	36.0	36.0	32.0	36.0
46	34.51529977540479	36.0	36.0	36.0	32.0	36.0
47	34.48613597287187	36.0	36.0	36.0	32.0	36.0
48	34.45923005016726	36.0	36.0	36.0	32.0	36.0
49	34.440668696446586	36.0	36.0	36.0	32.0	36.0
50	33.8762570528373	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	6.0
19	4.0
20	19.0
21	62.0
22	170.0
23	579.0
24	1424.0
25	3574.0
26	7194.0
27	13816.0
28	23268.0
29	36121.0
30	53357.0
31	75899.0
32	109674.0
33	175628.0
34	373025.0
35	1389805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.29036840024122	18.34777671989697	13.119881949338064	26.241972930523737
2	15.393032825811808	20.482325837211718	37.73380190535935	26.390839431617124
3	17.973045864969563	24.172532263543886	28.969797532539648	28.884624338946903
4	12.791280192681837	17.00676966356663	35.61247696176227	34.58947318198927
5	14.469780370272852	36.905313063807306	33.50775834191837	15.11714822400147
6	33.64868297531614	35.62498205312276	17.18858026395715	13.537754707603955
7	30.203310970981956	30.70308434343836	20.72595031157974	18.367654373999944
8	27.974737898629986	33.59500766026927	19.23323973727132	19.197014703829428
9	27.36634704376426	15.21533129675893	18.559554339206706	38.8587673202701
10	15.928885307590393	27.80672120784004	31.91718895163524	24.347204532934324
11	35.81401184293532	21.93359509601401	22.872486115209743	19.379906945840926
12	24.766414593223438	23.952455043368474	28.955092404840727	22.326037958567362
13	29.486379769024634	20.589163012487088	25.167320634488682	24.757136583999596
14	23.467501669888637	19.72929249734055	25.671003664919617	31.132202167851197
15	24.6924706784642	27.806729683449348	22.551251941662645	24.949547696423807
16	25.036899007824225	26.549879815922434	24.095129419321605	24.318091756931732
17	23.94558646562068	26.524764669813237	25.416411177101537	24.113237687464547
18	24.2215445457425	26.145765825225737	26.18093078466896	23.451758844362804
19	25.25485636332472	25.529150549471908	25.654480329806837	23.56151275739653
20	25.744542115544846	25.28761630778501	25.370713807968347	23.597127768701796
21	25.804151565539925	24.8779834849189	25.06432152279438	24.2535434267468
22	25.50913842733877	25.175161289752555	25.185896269174968	24.129804013733704
23	24.878667261586582	25.323222639942394	25.410163411541742	24.387946686929286
24	24.67366373095083	25.347585456190448	25.54161129802935	24.43713951482937
25	24.747922325087426	25.280704792568258	25.591847986471034	24.379524895873278
26	24.537772134584852	25.689487138026436	25.524653478206698	24.248087249182017
27	24.72263849519676	25.649798186149418	25.220722854093182	24.406840464560638
28	24.811511223619746	25.396968451931272	25.415876970925943	24.37564335352304
29	24.7248819786762	25.477276201264022	25.327689750810002	24.470152069249778
30	24.6724805944697	25.594756862069424	25.298582302863228	24.43418024059765
31	24.932713276381875	25.151892157451815	25.218781257621114	24.696613308545192
32	24.72186548375283	25.57331074946887	25.27939359319798	24.425430173580324
33	24.638605714538166	25.438773594283255	25.517191106791397	24.405429584387186
34	24.78542687549369	25.22871012262079	25.65184814743475	24.33401485445077
35	24.687142019653052	25.431100559426785	25.62795799726715	24.25379942365302
36	24.722338195369467	25.430547547885862	25.512457725026565	24.334656531718103
37	24.9573677383784	25.19924507763048	25.204723212802577	24.638663971188542
38	24.79136890889772	25.425093165506564	25.280358038300026	24.503179887295694
39	25.07917924838803	25.26044439339352	25.22090447022025	24.4394718879982
40	24.997426630246306	25.144848688067384	25.38561890536582	24.47210577632049
41	24.74345630230717	25.33628562542411	25.506902425921734	24.41335564634698
42	24.926786361963632	25.322575158565126	25.441457584243725	24.309180895227517
43	24.710205527775166	25.09653925384799	25.579887143723656	24.613368074653195
44	24.487716867704073	25.243992733672854	25.56140859306801	24.706881805555064
45	24.725326184819362	25.135293696661147	25.411182394015885	24.728197724503598
46	24.712748028034927	25.04363614676589	25.42554906885256	24.818066756346624
47	24.7053116791204	25.142583997547245	25.53567847010353	24.616425853228826
48	24.548016059544718	25.49002109935783	25.279822722983457	24.682140118113992
49	24.604924156747433	25.14132255647601	25.415132248579265	24.838621038197292
50	24.43802789461524	25.51984877126654	25.386962574069255	24.655160760048965
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	14.5
2	25.0
3	85.5
4	146.0
5	181.0
6	216.0
7	213.5
8	211.0
9	287.5
10	364.0
11	532.0
12	700.0
13	1315.5
14	1931.0
15	2569.5
16	3208.0
17	3147.0
18	3086.0
19	3115.0
20	3144.0
21	3835.5
22	4527.0
23	5867.5
24	7208.0
25	9384.0
26	11560.0
27	15162.5
28	18765.0
29	23495.5
30	28226.0
31	34473.0
32	40720.0
33	47977.0
34	55234.0
35	64710.5
36	74187.0
37	82867.5
38	91548.0
39	100319.0
40	109090.0
41	117701.0
42	126312.0
43	134876.0
44	143440.0
45	151686.5
46	159933.0
47	165925.5
48	171918.0
49	177357.5
50	182797.0
51	183249.5
52	183702.0
53	180126.0
54	176550.0
55	168654.5
56	160759.0
57	150652.0
58	140545.0
59	127950.5
60	115356.0
61	99708.0
62	84060.0
63	72391.5
64	60723.0
65	49604.5
66	38486.0
67	31694.0
68	24902.0
69	20149.5
70	15397.0
71	12156.0
72	8915.0
73	7562.0
74	6209.0
75	5012.0
76	3815.0
77	3104.5
78	2394.0
79	1938.0
80	1482.0
81	1169.5
82	857.0
83	661.5
84	466.0
85	350.0
86	234.0
87	177.5
88	121.0
89	92.0
90	63.0
91	40.5
92	18.0
93	19.0
94	20.0
95	21.0
96	22.0
97	20.5
98	19.0
99	16.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008526135919859623
2	5.742993106641198E-4
3	2.208843502554307E-4
4	0.0
5	0.0
6	1.325306101532584E-4
7	8.835374010217226E-5
8	0.0
9	0.001016068011174981
10	7.510067908684643E-4
11	0.0
12	8.835374010217226E-5
13	4.417687005108614E-4
14	1.767074802043445E-4
15	0.004594394485312958
16	7.510067908684643E-4
17	0.0
18	4.8594557056194746E-4
19	0.0
20	9.718911411238949E-4
21	0.0
22	0.0
23	7.951836609195504E-4
24	5.301224406130336E-4
25	0.0017670748020434455
26	0.005919700586845542
27	0.006891591727969436
28	0.004329333265006441
29	0.002385550982758651
30	0.004329333265006441
31	0.007951836609195505
32	0.002871496553320599
33	0.0045502176152618715
34	0.002827319683269512
35	0.002694789073116254
36	0.007642598518837901
37	0.003710857084291235
38	0.008084367219348762
39	0.00410844891475101
40	0.002606435333014082
41	0.0033574421238825457
42	0.0020763128924010483
43	0.0018112516720945313
44	0.0015903673218391008
45	0.0015020135817369286
46	0.001016068011174981
47	0.0022088435025543066
48	0.002297197242656479
49	5.301224406130336E-4
50	0.001634544191890187
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2263628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.34424264444807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.39597601114078	25.5742186296401
2	16.3133359698437	13.815517132949285
3	7.91809230581232	10.058568656353625
4	4.63048662899697	7.842977975204804
5	2.903779135063921	6.147916414051611
6	1.914556959507974	4.864227867001343
7	1.305578731905515	3.869861983066656
8	0.971438139222227	3.290784982503768
9	0.7164099962977926	2.7302254844527685
>10	2.8541171192212333	19.02485849387792
>50	0.0612846809627153	1.7148127851512582
>100	0.014839235368357517	1.0258922654983986
>500	1.0508665654432199E-4	0.040137330248327045
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	4.4176870051086134E-5	0.0
24	0.0	0.0	0.0	1.7670748020434454E-4	0.0
25	0.0	0.0	0.0	1.7670748020434454E-4	0.0
26	0.0	0.0	0.0	3.0923809035760295E-4	0.0
27	0.0	0.0	0.0	3.534149604086891E-4	0.0
28	0.0	0.0	0.0	7.951836609195504E-4	0.0
29	0.0	0.0	0.0	0.0021204897624521346	0.0
30	0.0	0.0	0.0	0.004771101965517302	0.0
31	0.0	0.0	0.0	0.01382736032598996	0.0
32	0.0	0.0	0.0	0.02385550982758651	0.0
33	0.0	0.0	0.0	0.035606557261175426	0.0
34	0.0	0.0	0.0	0.04846202644604149	0.0
35	0.0	0.0	0.0	0.06449823027458575	0.0
36	0.0	0.0	0.0	0.09051840673467548	0.0
37	0.0	0.0	0.0	0.13138201153193016	0.0
38	0.0	0.0	0.0	0.1940248132643703	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2685	0.0	29.827124	1
GGTATCA	930	0.0	27.206282	1
TATCAAC	3945	0.0	19.906677	2
TAGGACG	510	0.0	19.841513	4
TCAACGC	4050	0.0	19.336689	4
ATCAACG	4115	0.0	19.030828	3
CAACGCA	4205	0.0	18.676237	5
AACGCAG	4350	0.0	18.104265	6
GTATAGG	325	0.0	16.924309	1
GTAGGAC	780	0.0	16.639296	3
TTAGGAC	555	0.0	16.250563	3
TATACCG	95	3.3500153E-4	16.2092	5
GTCCTAC	765	0.0	15.24294	1
ACGCAGA	5310	0.0	14.831179	7
AGAGTAC	5430	0.0	14.422711	11
CGCAGAG	5510	0.0	14.412614	8
GTATAGA	400	0.0	14.301042	1
TAGGACC	680	0.0	14.23413	4
CAGAGTA	5620	0.0	14.131141	10
CTAGTAT	190	1.7571074E-7	13.893292	3
>>END_MODULE