FastQCFastQC Report
Thu 2 Feb 2017
SRR4063899_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063899_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347000
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT81390.6042316258351893No Hit
GTCCTACAGTGGACATTTCTAAATT68740.5103192279138827No Hit
CTTTAGGACGTGAAATATGGCGAGG68740.5103192279138827No Hit
CTGTAGGACGTGGAATATGGCAAGA63780.4734966592427617No Hit
GTCCTACAGTGTGCATTTCTCATTT46180.3428359317000742No Hit
GTATCAACGCAGAGTACTTTTTTTT41340.3069042316258352No Hit
CTGTAGGACCTGGAATATGGCGAGA34110.2532293986636971No Hit
CTGAAGGACCTGGAATATGGCGAGA33760.2506310319227914No Hit
ATTTAGAAATGTCCACTGTAGGACG31500.23385300668151446No Hit
TTTCTAAATTTTCCACCTTTTTCAG28130.20883444691907946No Hit
GTCCTTCAGTGTGCATTTCTCATTT26660.19792130660727542No Hit
TATCAACGCAGAGTACTTTTTTTTT26560.1971789161098738No Hit
GAATATGGCAAGAAAACTGAAAATC23610.17527839643652562No Hit
GGAATATGGCGAGAAAACTGAAAAT18460.1370452858203415No Hit
GAAATATGGCGAGGAAAACTGAAAA16390.12167780252412769No Hit
GCCATATTCCACGTCCTACAGTGGA16370.12152932442464737No Hit
GGTATCAACGCAGAGTACTTTTTTT15530.11529324424647365No Hit
CCATATTTCACGTCCTAAAGTGTGT14680.10898292501855976No Hit
ATTCCAGGTCCTTCAGTGTGCATTT14540.10794357832219748No Hit
CTGTAGGACATGGAATATGGCAAGA14360.10660727542687452No Hit
CCATATTCCAGGTCCTTCAGTGTGC14160.10512249443207126No Hit
TTCCAGGTCCTTCAGTGTGCATTTC13990.10386043058648849No Hit
ACAGTGGACATTTCTAAATTTTCCA13630.10118782479584261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1500.013.93821119
ACCTGGC500.001498768813.3007038
TAGGACC11700.012.7466424
GGCGAAG604.0793276E-412.67110219
TCCAACG1950.012.67063118
TATTGCG905.385118E-712.67015916
CCACCTT7650.012.66592413
GTATCAA36000.012.6094021
AAATGTC10150.012.3553327
TTGCGAG1001.435983E-712.35386618
CACCTTT7700.012.33923114
GGTATCA12250.012.2487031
ACCTTTT8200.012.16798915
ATTGCGA951.0354961E-612.00375617
TATGGAC802.8717752E-511.87253953
TATAGCG658.003658E-411.69553216
ATTTAGA11550.011.6754981
TCCACCT8450.011.578341512
TTCCACC8800.011.54960211
CCCTTAT752.0802859E-411.3959441
Produced by FastQC (version 0.11.5)