##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063899_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1347000
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32598292501856	32.0	32.0	32.0	32.0	32.0
2	30.9920282108389	32.0	32.0	32.0	32.0	32.0
3	31.023412769116554	32.0	32.0	32.0	32.0	32.0
4	31.03410838901262	32.0	32.0	32.0	32.0	32.0
5	31.015170749814402	32.0	32.0	32.0	32.0	32.0
6	34.691665181885675	36.0	36.0	36.0	32.0	36.0
7	34.60695694135115	36.0	36.0	36.0	32.0	36.0
8	34.58298589458055	36.0	36.0	36.0	32.0	36.0
9	34.657870824053454	36.0	36.0	36.0	32.0	36.0
10	34.43138752783965	36.0	36.0	36.0	32.0	36.0
11	34.66300148478099	36.0	36.0	36.0	32.0	36.0
12	34.516606533036374	36.0	36.0	36.0	32.0	36.0
13	34.559575352635484	36.0	36.0	36.0	32.0	36.0
14	34.503313288789904	36.0	36.0	36.0	32.0	36.0
15	34.47455456570156	36.0	36.0	36.0	32.0	36.0
16	34.47190571640683	36.0	36.0	36.0	32.0	36.0
17	34.432855233853005	36.0	36.0	36.0	32.0	36.0
18	34.4311425389755	36.0	36.0	36.0	32.0	36.0
19	34.379529324424645	36.0	36.0	36.0	32.0	36.0
20	34.34469933184855	36.0	36.0	36.0	32.0	36.0
21	34.32444172234595	36.0	36.0	36.0	32.0	36.0
22	34.28481811432814	36.0	36.0	36.0	32.0	36.0
23	34.276705270972535	36.0	36.0	36.0	32.0	36.0
24	34.24958203414996	36.0	36.0	36.0	32.0	36.0
25	33.89418337045286	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	14.0
5	14.0
6	53.0
7	21.0
8	92.0
9	102.0
10	139.0
11	37.0
12	67.0
13	81.0
14	150.0
15	251.0
16	380.0
17	498.0
18	629.0
19	815.0
20	1194.0
21	1536.0
22	2536.0
23	3769.0
24	5706.0
25	8304.0
26	12023.0
27	16254.0
28	22336.0
29	30955.0
30	41278.0
31	57256.0
32	81838.0
33	119492.0
34	272629.0
35	666547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32529458335004	21.215942629586223	13.4838445188113	23.974918268252434
2	11.484349478810916	21.98029578594126	47.43867490274107	19.096679832506755
3	17.05598491428911	29.479721151918753	30.82422028701456	22.64007364677758
4	9.906180421352882	18.811950284606596	41.63452608009076	29.647343213949767
5	9.88303932916711	41.92067047650726	36.76632292965209	11.429967264673541
6	26.73526909089289	41.11445402934673	20.976333425898837	11.173943453861542
7	25.489415641844953	33.676132823725716	24.153886253143895	16.680565281285435
8	22.96274400876961	41.4074452567755	21.20184066758363	14.427970066871252
9	27.37632418283565	15.18797416924286	21.883500135927896	35.552201511993594
10	14.31772074133998	31.00812163811502	35.80317897540925	18.870978645135747
11	31.740742363949835	22.713716180128998	28.65438084463603	16.891160611285137
12	24.26985473611955	25.75317472227216	34.46031578587655	15.516654755731734
13	28.740323611131746	24.9336582321739	27.26557508580598	19.06044307088837
14	18.99714271704875	25.394157948461928	29.772799201208883	25.835900133280436
15	19.696801986751524	38.0660076352947	24.008982874445532	18.22820750350824
16	17.84170064307535	29.87685285342399	33.38449605952904	18.896950443971612
17	18.01349702913807	32.51076835300288	30.623204964957353	18.852529652901698
18	17.951747161127866	28.881934274435327	36.25226526504437	16.914053299392435
19	22.767194756891016	27.03661958854879	29.684995902428007	20.511189752132186
20	23.75712364492856	28.805159487914878	30.721986521729438	16.715730345427122
21	21.61397288548139	27.620268945948535	28.184343657614374	22.581414510955693
22	22.3109129748305	32.31919754806353	28.541803659329435	16.82808581777654
23	18.612314834629192	32.18385167748532	30.82676486672849	18.377068621157
24	21.670371702245905	28.5496271684117	32.11136504804163	17.668636081300765
25	19.730463908793432	30.491177440878303	31.668707480971385	18.109651169356876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	134.0
1	134.0
2	339.0
3	544.0
4	544.0
5	544.0
6	1374.0
7	2204.0
8	2204.0
9	2204.0
10	2797.5
11	3391.0
12	3391.0
13	3391.0
14	5170.0
15	6949.0
16	6949.0
17	6949.0
18	13028.5
19	19108.0
20	19108.0
21	19108.0
22	33578.0
23	48048.0
24	48048.0
25	48048.0
26	75001.5
27	101955.0
28	101955.0
29	101955.0
30	135007.0
31	168059.0
32	168059.0
33	168059.0
34	180927.5
35	193796.0
36	193796.0
37	193796.0
38	197086.5
39	200377.0
40	200377.0
41	200377.0
42	195873.5
43	191370.0
44	191370.0
45	191370.0
46	182751.5
47	174133.0
48	174133.0
49	174133.0
50	149561.5
51	124990.0
52	124990.0
53	124990.0
54	92561.0
55	60132.0
56	60132.0
57	60132.0
58	44495.5
59	28859.0
60	28859.0
61	28859.0
62	20905.5
63	12952.0
64	12952.0
65	12952.0
66	9455.0
67	5958.0
68	5958.0
69	5958.0
70	4022.0
71	2086.0
72	2086.0
73	2086.0
74	1455.0
75	824.0
76	824.0
77	824.0
78	571.5
79	319.0
80	319.0
81	319.0
82	225.0
83	131.0
84	131.0
85	131.0
86	109.5
87	88.0
88	88.0
89	88.0
90	70.0
91	52.0
92	52.0
93	52.0
94	44.5
95	37.0
96	37.0
97	37.0
98	270.5
99	504.0
100	504.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03875278396436525
2	0.005939123979213066
3	0.0022271714922048997
4	0.003934669636228657
5	0.010170749814402375
6	0.015293244246473645
7	0.025463994060876023
8	0.03949517446176689
9	0.051818856718634
10	0.06258351893095769
11	0.05842613214550854
12	0.06666666666666667
13	0.06978470675575352
14	0.07171492204899778
15	0.06555308092056421
16	0.0718634001484781
17	0.06874536005939123
18	0.08396436525612472
19	0.08025241276911656
20	0.08389012620638456
21	0.08010393466963622
22	0.08351893095768374
23	0.08730512249443206
24	0.08047512991833705
25	0.08403860430586488
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1347000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.23007584139789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.88837418311151	48.50254613394464
2	12.171447539723346	14.41831521754819
3	2.9617113008348426	5.262671549063188
4	1.0653535633922395	2.524038894305034
5	0.5002736481490255	1.4815623060659795
6	0.28740797532392665	1.0213917705515274
7	0.1811788870256761	0.75118674535736
8	0.12409305514689786	0.5880032854193226
9	0.09422944187508438	0.5023095289878458
>10	0.6029694187386773	6.848644866983646
>50	0.06279305890087668	2.5655874415607105
>100	0.048689377898645594	6.086761789105368
>500	0.00643303344885448	2.505527553889026
>1k	0.004540964787426692	4.829730102610809
>5k	5.045516430474102E-4	2.111722814607387
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	8139	0.6042316258351893	No Hit
GTCCTACAGTGGACATTTCTAAATT	6874	0.5103192279138827	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6874	0.5103192279138827	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6378	0.4734966592427617	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4618	0.3428359317000742	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4134	0.3069042316258352	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3411	0.2532293986636971	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3376	0.2506310319227914	No Hit
ATTTAGAAATGTCCACTGTAGGACG	3150	0.23385300668151446	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2813	0.20883444691907946	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2666	0.19792130660727542	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2656	0.1971789161098738	No Hit
GAATATGGCAAGAAAACTGAAAATC	2361	0.17527839643652562	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1846	0.1370452858203415	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1639	0.12167780252412769	No Hit
GCCATATTCCACGTCCTACAGTGGA	1637	0.12152932442464737	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1553	0.11529324424647365	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1468	0.10898292501855976	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1454	0.10794357832219748	No Hit
CTGTAGGACATGGAATATGGCAAGA	1436	0.10660727542687452	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1416	0.10512249443207126	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1399	0.10386043058648849	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1363	0.10118782479584261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGA	150	0.0	13.938211	19
ACCTGGC	50	0.0014987688	13.300703	8
TAGGACC	1170	0.0	12.746642	4
GGCGAAG	60	4.0793276E-4	12.671102	19
TCCAACG	195	0.0	12.670631	18
TATTGCG	90	5.385118E-7	12.670159	16
CCACCTT	765	0.0	12.665924	13
GTATCAA	3600	0.0	12.609402	1
AAATGTC	1015	0.0	12.355332	7
TTGCGAG	100	1.435983E-7	12.353866	18
CACCTTT	770	0.0	12.339231	14
GGTATCA	1225	0.0	12.248703	1
ACCTTTT	820	0.0	12.167989	15
ATTGCGA	95	1.0354961E-6	12.003756	17
TATGGAC	80	2.8717752E-5	11.8725395	3
TATAGCG	65	8.003658E-4	11.695532	16
ATTTAGA	1155	0.0	11.675498	1
TCCACCT	845	0.0	11.5783415	12
TTCCACC	880	0.0	11.549602	11
CCCTTAT	75	2.0802859E-4	11.395944	1
>>END_MODULE