FastQCFastQC Report
Thu 2 Feb 2017
SRR4063899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347000
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA34610.25694135115070527No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA30770.22843355605048254No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA30050.2230883444691908No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC25890.19220489977728286No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA19930.14795842613214552No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA16130.11974758723088344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGC407.034946E-427.498419
GTATCAA34700.026.757461
TGTACGA609.758462E-625.6651742
TAGGACC16300.024.4280244
CTGTACG551.592774E-424.0021671
GTACGAC551.5943791E-423.9986043
TGCGAGG651.8144085E-523.69092819
CGGACAT952.6864655E-823.15740810
GGTATCA13700.022.0019861
TATCCGT803.617879E-621.998726
ATCCGTA703.2171512E-521.9987187
AGGACCT29850.021.1511965
GCGGACA1057.7494406E-820.9519399
GGACCTG30100.020.0984976
GTCACGG550.004483456320.0003235
TACGGCG550.00448538319.99883716
AGCGGAC1101.2666715E-719.9988378
GTCCTAC45950.019.3923911
GACCTGG30950.019.3332847
TGTAGGA50700.019.308542
Produced by FastQC (version 0.11.5)