##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063899_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1347000
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.360415738678544	32.0	32.0	32.0	32.0	32.0
2	31.49727988121752	32.0	32.0	32.0	32.0	32.0
3	31.557640682999256	32.0	32.0	32.0	32.0	32.0
4	31.62440311804009	32.0	32.0	32.0	32.0	32.0
5	31.606479584261322	32.0	32.0	32.0	32.0	32.0
6	35.21101855976244	36.0	36.0	36.0	36.0	36.0
7	35.22321455085375	36.0	36.0	36.0	36.0	36.0
8	35.174740905716405	36.0	36.0	36.0	36.0	36.0
9	35.27262583518931	36.0	36.0	36.0	36.0	36.0
10	35.171728285077954	36.0	36.0	36.0	36.0	36.0
11	35.27386265775798	36.0	36.0	36.0	36.0	36.0
12	35.21948329621381	36.0	36.0	36.0	36.0	36.0
13	35.240802524127695	36.0	36.0	36.0	36.0	36.0
14	35.197521900519675	36.0	36.0	36.0	36.0	36.0
15	35.18156347438753	36.0	36.0	36.0	36.0	36.0
16	35.21220267260579	36.0	36.0	36.0	36.0	36.0
17	35.20477728285078	36.0	36.0	36.0	36.0	36.0
18	35.18890794357832	36.0	36.0	36.0	36.0	36.0
19	35.16221677802524	36.0	36.0	36.0	36.0	36.0
20	35.149970304380105	36.0	36.0	36.0	36.0	36.0
21	35.12972383073497	36.0	36.0	36.0	36.0	36.0
22	35.10030512249443	36.0	36.0	36.0	36.0	36.0
23	35.084995545657016	36.0	36.0	36.0	36.0	36.0
24	35.0725152190052	36.0	36.0	36.0	36.0	36.0
25	35.034052709725316	36.0	36.0	36.0	36.0	36.0
26	34.993704528582036	36.0	36.0	36.0	36.0	36.0
27	34.940424647364516	36.0	36.0	36.0	36.0	36.0
28	34.896544914625096	36.0	36.0	36.0	36.0	36.0
29	34.878533036377135	36.0	36.0	36.0	36.0	36.0
30	34.8225991091314	36.0	36.0	36.0	36.0	36.0
31	34.81429621380846	36.0	36.0	36.0	36.0	36.0
32	34.779375649591685	36.0	36.0	36.0	32.0	36.0
33	34.808612472160355	36.0	36.0	36.0	36.0	36.0
34	34.77927765404603	36.0	36.0	36.0	36.0	36.0
35	34.764017817371936	36.0	36.0	36.0	36.0	36.0
36	34.69916035634744	36.0	36.0	36.0	32.0	36.0
37	34.622633259094286	36.0	36.0	36.0	32.0	36.0
38	34.58444394951744	36.0	36.0	36.0	32.0	36.0
39	34.4431700074239	36.0	36.0	36.0	32.0	36.0
40	34.512053452115815	36.0	36.0	36.0	32.0	36.0
41	34.485780252412766	36.0	36.0	36.0	32.0	36.0
42	34.47211135857461	36.0	36.0	36.0	32.0	36.0
43	34.46497624350408	36.0	36.0	36.0	32.0	36.0
44	34.36451150705271	36.0	36.0	36.0	32.0	36.0
45	34.32573793615442	36.0	36.0	36.0	32.0	36.0
46	34.1857713437268	36.0	36.0	36.0	32.0	36.0
47	34.2008292501856	36.0	36.0	36.0	32.0	36.0
48	34.14854491462509	36.0	36.0	36.0	32.0	36.0
49	34.09302227171492	36.0	36.0	36.0	32.0	36.0
50	33.64786340014848	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	13.0
21	35.0
22	99.0
23	344.0
24	897.0
25	2006.0
26	4164.0
27	8004.0
28	14230.0
29	21434.0
30	31977.0
31	45420.0
32	65687.0
33	104686.0
34	218199.0
35	829798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.58066204612866	22.10745535067953	13.914105510121502	24.397777093070307
2	11.156001089831676	22.84147626163978	46.56446896318255	19.438053685345995
3	16.67009405394089	29.954424410520318	31.183606744801196	22.191874790737593
4	9.830141054194506	19.556124721603563	41.620415738678545	28.993318485523385
5	9.740237564959168	42.357089829250185	36.41974758723088	11.482925018559763
6	26.992285055040995	41.312297883586886	20.572444164644885	11.122972896727235
7	25.483334050978545	33.6837916611606	24.274052374242576	16.55882191361828
8	22.983090559087273	41.62074359372204	21.009072761004276	14.387093086186404
9	27.07445852864709	15.21843712369467	21.892859423093768	35.81424492456447
10	14.211240973345618	31.32533966649913	35.60839297897598	18.85502638117927
11	31.743207126948775	22.726503340757237	28.751373422420194	16.778916109873794
12	24.33730413853621	25.926616075153785	34.124029879777105	15.6120499065329
13	28.9175749854862	24.809687348273265	27.155874487933872	19.11686317830666
14	19.00596734367831	25.568189410823177	29.59766829646369	25.828174949034814
15	19.646587860060613	38.17499996287844	23.935093687403764	18.24331848965719
16	17.90004536036247	30.049124307437836	33.031846537950145	19.018983794249554
17	18.089086859688198	32.79354120267261	30.342538975501114	18.774832962138085
18	18.038758895733757	28.97741192611103	36.05086585389393	16.93296332426128
19	22.937210792287154	27.082351211842028	29.683169772212153	20.297268223658666
20	23.965582521028367	28.976161664154894	30.450188939784262	16.608066875032478
21	21.661618411284337	27.642093541202673	28.206236080178176	22.490051967334818
22	22.599976094972742	32.094280833587604	28.52003382338639	16.785709248053262
23	18.559872664425626	32.32877403874708	30.667219998337035	18.444133298490264
24	21.92873767163204	28.58065545900319	31.771387421631115	17.719219447733657
25	19.944408726523303	30.585519839284203	31.254055386369746	18.21601604782275
26	17.251701649976837	32.02658197023152	32.10141893256358	18.62029744722806
27	18.605652583219246	31.582108567372767	30.546935090621297	19.265303758786686
28	17.784847247485057	31.960800326663946	29.516314636771966	20.73803778907903
29	20.232536903224943	29.761041271179963	28.936152205433086	21.07026962016201
30	18.35626527053202	29.814685992203167	31.012942506702146	20.816106230562667
31	21.58192727969269	28.462247775284837	30.327103289445358	19.62872165557712
32	19.08274930213221	31.45111955811605	30.413152580626	19.052978559125737
33	17.737355199240945	32.18086666265757	32.20091214362662	17.88086599447487
34	19.263606464880436	31.35340801948076	31.9895023645664	17.393483151072406
35	18.33769747201668	32.16428365144402	31.250450086602232	18.247568789937066
36	17.652762578198434	30.80665590610694	32.84601460842501	18.694566907269614
37	18.285601014435514	30.723349537772577	29.21119917799839	21.779850269793517
38	19.174409669504016	32.89331546534117	29.27393339594555	18.658341469209265
39	21.32887588180181	29.297350028731405	30.10086431796686	19.272909771499926
40	22.136961405457253	28.94917363164061	31.069920963816784	17.84394399908536
41	21.734885768609143	28.00270832297048	31.95479595147291	18.307609956947466
42	19.694621012010554	31.709926339030513	31.22157534399123	17.373877304967696
43	17.759029776063333	32.2801687914072	31.612531069319104	18.34827036321036
44	17.467193471656763	31.830201902318358	30.777928236925646	19.924676389099236
45	17.956695880058053	32.12931101682647	30.320604906513438	19.593388196602042
46	18.937779790495846	29.229170223980876	30.921461926220683	20.911588059302595
47	21.47945278703105	29.3311437291523	30.011485028259706	19.17791845555695
48	17.94408842272811	30.601551317610305	30.19790880960724	21.256451450054346
49	20.66738084791577	29.294942750429286	29.84891577338331	20.18876062827163
50	18.40278963552702	32.94510856089076	29.12843934532356	19.523662458258663
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	22.0
2	19.0
3	56.5
4	94.0
5	125.5
6	157.0
7	197.5
8	238.0
9	294.0
10	350.0
11	536.0
12	722.0
13	1414.0
14	2106.0
15	2994.5
16	3883.0
17	4282.0
18	4681.0
19	5066.5
20	5452.0
21	6495.5
22	7539.0
23	9693.5
24	11848.0
25	15289.5
26	18731.0
27	26306.5
28	33882.0
29	45007.5
30	56133.0
31	71202.0
32	86271.0
33	106824.5
34	127378.0
35	147356.0
36	167334.0
37	165613.0
38	163892.0
39	152238.0
40	140584.0
41	127692.5
42	114801.0
43	104442.0
44	94083.0
45	86162.5
46	78242.0
47	71396.5
48	64551.0
49	57675.5
50	50800.0
51	44441.0
52	38082.0
53	32299.0
54	26516.0
55	22114.5
56	17713.0
57	14767.5
58	11822.0
59	9704.0
60	7586.0
61	6079.0
62	4572.0
63	3612.0
64	2652.0
65	2144.5
66	1637.0
67	1402.0
68	1167.0
69	909.5
70	652.0
71	479.5
72	307.0
73	253.0
74	199.0
75	163.5
76	128.0
77	99.5
78	71.0
79	54.0
80	37.0
81	29.0
82	21.0
83	17.0
84	13.0
85	9.5
86	6.0
87	6.0
88	6.0
89	6.5
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	2.5
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008537490720118782
2	2.2271714922048998E-4
3	5.196733481811432E-4
4	0.0
5	0.0
6	2.969561989606533E-4
7	1.4847809948032666E-4
8	7.423904974016333E-5
9	0.001633259094283593
10	0.0012620638455827764
11	0.0
12	1.4847809948032666E-4
13	4.4543429844097997E-4
14	1.4847809948032666E-4
15	0.005493689680772086
16	6.681514476614699E-4
17	0.0
18	4.4543429844097997E-4
19	7.423904974016333E-5
20	7.423904974016332E-4
21	0.0
22	2.2271714922048998E-4
23	5.939123979213066E-4
24	3.711952487008166E-4
25	0.0019302152932442465
26	0.005345211581291759
27	0.005642167780252413
28	0.0037119524870081666
29	0.0023014105419450633
30	0.003192279138827023
31	0.006384558277654046
32	0.002969561989606533
33	0.004677060133630289
34	0.0022271714922048997
35	0.002746844840386043
36	0.007869339272457313
37	0.003266518188567186
38	0.006310319227913883
39	0.00311804008908686
40	0.001633259094283593
41	0.003043801039346696
42	0.0013363028953229399
43	8.908685968819599E-4
44	8.908685968819599E-4
45	0.0011135857461024498
46	7.423904974016332E-4
47	0.0021529324424647365
48	0.0011878247958426132
49	2.2271714922048998E-4
50	0.0010393466963622864
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1347000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.53618713056832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.43884192087766	59.68896609609813
2	11.574085485534932	16.559318903168435
3	2.725744672619053	5.849681429118789
4	0.9303558475667366	2.6621644003821032
5	0.40671439242685997	1.4547398442671629
6	0.217502695980597	0.933558813664266
7	0.13669527372317306	0.6845061076647616
8	0.09405634237231443	0.538274568701015
9	0.06885189782971592	0.4432862022697196
>10	0.35755938172058677	4.672484680420457
>50	0.02783242804761893	1.3904978560151313
>100	0.018412070703709114	2.5002074148111766
>500	0.002406080741580538	1.18928709388689
>1k	9.4150985540108E-4	1.4330265895318437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	3461	0.25694135115070527	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	3077	0.22843355605048254	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	3005	0.2230883444691908	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2589	0.19220489977728286	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1993	0.14795842613214552	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1613	0.11974758723088344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	1.4847809948032666E-4	0.0
15	0.0	0.0	0.0	2.2271714922048998E-4	0.0
16	0.0	0.0	0.0	2.969561989606533E-4	0.0
17	0.0	0.0	0.0	2.969561989606533E-4	0.0
18	0.0	0.0	0.0	2.969561989606533E-4	0.0
19	0.0	0.0	0.0	4.4543429844097997E-4	0.0
20	0.0	0.0	0.0	5.939123979213066E-4	0.0
21	0.0	0.0	0.0	7.423904974016332E-4	0.0
22	0.0	0.0	0.0	9.651076466221232E-4	0.0
23	0.0	0.0	0.0	0.0017817371937639199	0.0
24	0.0	0.0	0.0	0.0026726057906458797	0.0
25	0.0	0.0	0.0	0.003192279138827023	0.0
26	0.0	0.0	0.0	0.0032665181885671865	0.0
27	0.0	0.0	0.0	0.003637713437268003	0.0
28	0.0	0.0	0.0	0.005716406829992576	0.0
29	0.0	0.0	0.0	0.008314773570898293	0.0
30	0.0	0.0	0.0	0.014179658500371195	0.0
31	0.0	0.0	0.0	0.030883444691907944	0.0
32	0.0	0.0	0.0	0.052264291017074985	0.0
33	0.0	0.0	0.0	0.07401633259094284	0.0
34	0.0	0.0	0.0	0.09970304380103935	0.0
35	0.0	0.0	0.0	0.12962138084632516	0.0
36	7.423904974016333E-5	0.0	0.0	0.17082405345211582	0.0
37	7.423904974016333E-5	0.0	0.0	0.2315515961395694	0.0
38	7.423904974016333E-5	0.0	0.0	0.3149220489977728	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGAGC	40	7.034946E-4	27.4984	19
GTATCAA	3470	0.0	26.75746	1
TGTACGA	60	9.758462E-6	25.665174	2
TAGGACC	1630	0.0	24.428024	4
CTGTACG	55	1.592774E-4	24.002167	1
GTACGAC	55	1.5943791E-4	23.998604	3
TGCGAGG	65	1.8144085E-5	23.690928	19
CGGACAT	95	2.6864655E-8	23.157408	10
GGTATCA	1370	0.0	22.001986	1
TATCCGT	80	3.617879E-6	21.99872	6
ATCCGTA	70	3.2171512E-5	21.998718	7
AGGACCT	2985	0.0	21.151196	5
GCGGACA	105	7.7494406E-8	20.951939	9
GGACCTG	3010	0.0	20.098497	6
GTCACGG	55	0.0044834563	20.00032	35
TACGGCG	55	0.004485383	19.998837	16
AGCGGAC	110	1.2666715E-7	19.998837	8
GTCCTAC	4595	0.0	19.392391	1
GACCTGG	3095	0.0	19.333284	7
TGTAGGA	5070	0.0	19.30854	2
>>END_MODULE