FastQCFastQC Report
Thu 2 Feb 2017
SRR4063898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT141.222707423580786No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT90.7860262008733625RNA PCR Primer, Index 47 (95% over 24bp)
GAGCAGAGCAGAAGGGGTAGCAGAGGGCACTGGCTGGAGGAGGCTGTGCA70.611353711790393No Hit
GTACATGGGGATTCCTCCCCTTTGTGACCACGTCCCAATCTAACTGAAGA60.5240174672489083No Hit
GCCCAGGAGAGTGTAGTGCCACAGAGCCGGGGGAACTGAAAGGGAGCATG60.5240174672489083No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT60.5240174672489083RNA PCR Primer, Index 47 (95% over 22bp)
CATTTGGATGCAGAAAGACGACGACATCATTAGATTCCAGCTGCATCTGT50.43668122270742354No Hit
GAGCCGGGGGAACTGAAAGGGAGCATGTTCGAATGAGCCCAGAGCCACAG50.43668122270742354No Hit
TATCAACGCAGAGTACATGGGGATTCCTCCCCTTTGTGACCACGTCCCAA40.34934497816593885No Hit
GTGCATCACAGAGGACGTTTTCTTCAGTTAGATTGGGACGTGGTCACAAA30.26200873362445415No Hit
GTCCCTGGCTCGGGGCAGGTTCCCTGCTCTGCTCTGCTGTCCCTCGAGCC30.26200873362445415No Hit
AGCAAGACACGACCTCGTGGACACCAGGGCTCGAGGGACAGCAGAGCAGA30.26200873362445415No Hit
GCTCTGCTCTGCTGTCCCTCGAGCCCTGGTGTCCACGAGGTCGTGTCTTG30.26200873362445415No Hit
GGACGGAGAGTCTCCAGCAAAGAGACTGCAGTGGCAGCTGTGGATGGGGG30.26200873362445415No Hit
CTGCTACCCCTTCTGCTCTGCTCACAGCAACCGCCGAGAGATGGGTCTGC30.26200873362445415No Hit
CACCAGGGGAATGTGCAGGGAGAGAAGACAAGCAAGACACGACCTCGTGG30.26200873362445415No Hit
CTGAAAGGGAGCATGTTCGAATGAGCCCAGAGCCACAGAAGCCACATGGG30.26200873362445415No Hit
CCCCACAGGCCTCTCGTTCCTCATTCTGTGGTCGTCCAGCTTCTGGTGCC30.26200873362445415No Hit
GGTCTGCACTTGCTGTCACCCCCTCCCCACAGGCCTCTCGTTCCTCATTC30.26200873362445415No Hit
TTCCCCTGGTGTGATTTCACCCCCATCCACAGCTGCCACTGCAGTCTCTT30.26200873362445415No Hit
GCCCAGAGCCACAGAAGCCACATGGGAGCCGGGCAGAAGCACGCCTGTGG30.26200873362445415No Hit
CTGAAACCCTCTTTTTCTAGAATCTGTAAGTGGACATTTGGAGGGCTTTG30.26200873362445415No Hit
GCTCACAGCAACCGCCGAGAGATGGGTCTGCACTTGCTGTCACCCCCTCC30.26200873362445415No Hit
CTCCAGGACATCTCCACTGACTGGCATTTCCTCACAGATGCAGCTGGAAT30.26200873362445415No Hit
GGAGAGTGTAGTGCCACAGAGCCGGGGGAACTGAAAGGGAGCATGTTCGA30.26200873362445415No Hit
CTCCCTTTCAGTTCCCCCGGCTCTGTGGCACTACACTCTCCTGGGCCACC30.26200873362445415No Hit
TCCTCATTCTGTGGTCGTCCAGCTTCTGGTGCCATCGTACCCCCTGGTCA30.26200873362445415No Hit
GTTCGAATGAGCCCAGAGCCACAGAAGCCACATGGGAGCCGGGCAGAAGC30.26200873362445415No Hit
GAGCAGGGAACCTGCCCCGAGCCAGGGACAGAGGGGACAAAGAGGAGGTG30.26200873362445415No Hit
GTGCAGGGAGAGAAGACAAGCAAGACACGACCTCGTGGACACCAGGGCTC30.26200873362445415No Hit
AAGCACGCCTGTGGAGCTAGGGGCGAGAGACCTCCAGTGACCAGGGGGTA30.26200873362445415No Hit
GTACATGGGGAGCACATTTGGATGCAGAAAGACGACGACATCATTAGATT30.26200873362445415No Hit
CCCATCTCTCGGCGGTTGCTGTGAGCAGAGCAGAAGGGGTAGCAGAGGGC30.26200873362445415No Hit
CCCTGGCTCGGGGCAGGTTCCCTGCTCTGCTCTGCTGTCCCTCGAGCCCT30.26200873362445415No Hit
CTGTGATGCACAGCCTCCTCCAGCCAGTGCCCTCTGCTACCCCTTCTGCT30.26200873362445415No Hit
TCTTTGTCCCCTCTGTCCCTGGCTCGGGGCAGGTTCCCTGCTCTGCTCTG30.26200873362445415No Hit
GGAATGTGCAGGGAGAGAAGACAAGCAAGACACGACCTCGTGGACACCAG30.26200873362445415No Hit
TCCCTGCTCTGCTCTGCTGTCCCTCGAGCCCTGGTGTCCACGAGGTCGTG30.26200873362445415No Hit
CCCAGAAGCTGGACGACCACAGAATGAGGAACGAGAGGCCTGTGGGGAGG20.17467248908296942No Hit
GCTCTGCTCTGCTGTCCCTCGAGCCCTGGTGTCCACCTGTCTCTTATACA20.17467248908296942No Hit
CCACAGGCGTGCTTCTGCCCGGCTCCCATGTGGCTTCTGTGGCTCTGGGC20.17467248908296942No Hit
ACGCAGAGTACATGGGGAGCACATTTGGATGCAGAAAGACGACGACATCA20.17467248908296942No Hit
GGCGTGCTTCTGCCCGGCTCCCATGTGGCTTCTGTGGCTCTGGGCTCATT20.17467248908296942No Hit
CATGGGAGCCGGGCAGAAGCACGCCTGTGGAGCTAGGGGCGAGAGACCTC20.17467248908296942No Hit
TATCTCTGCAGTGATCACACAGGTGATGTAACTCTTGTCTATATCTGCCT20.17467248908296942No Hit
GTATCACTCAGCGCCCCAGGCCGCGGTCGGCATCACCATCCGGACCGGCG20.17467248908296942No Hit
GGGCAAAGCGGCGCACATCGCGACTGGTCTACTTACAGCTCGTAAGGACT20.17467248908296942No Hit
TCAACGCAGAGTACATGGGGATTCCTCCCCTTTGTGACCACGTCCCAATC20.17467248908296942No Hit
GCCGACCGGGAGGTTCAGGCCTCGTACACGAAGCCGCCGTCGATCATGGT20.17467248908296942No Hit
CACTTGCTGTCACCCCCTCCCCACAGGCCTCTCGTTCCTCATTCTGTGGT20.17467248908296942No Hit
CTTTGCTGGAGACTCTCCGTCCTCTGCCCCAGTCCTGACCTCTCTCCCGA20.17467248908296942No Hit
GGTATTTAGGCCACAAACCATCCGGTTTTGTCCCTTTACGTAACAGGTTT20.17467248908296942No Hit
ATAATACTAAAGTAAAAAATTTCAAGTAACAACCCCATTTCTAATTAAAG20.17467248908296942No Hit
TTCCTCCCCTTTGTGACCACGTCCCAATCTAACTGAAGAAAACGTCCTCT20.17467248908296942No Hit
CTCCACAGGCGTGCTTCTGCCCGGCTCCCATGTGGCTTCTGTGGCTCTGG20.17467248908296942No Hit
GTGAGGAAATGCCAGTCAGTGGAGATGTCCTGGAGCTCGGGAGAGAGGTC20.17467248908296942No Hit
CGTCCCAATCTAACTGAAGAAAACGTCCTCTGTGATGCACAGCCTCCTCC20.17467248908296942No Hit
ATCCAGGGAGAGGCTGGAAAACTTACGTGTTCGGTTGGAGGAAGTCTTAC20.17467248908296942No Hit
GTATCAACGCAGAGTACATGGGGATTCCTCCCCTTTGTGACCACGTCCCA20.17467248908296942No Hit
TCTCCACTGACTGGCATTTCCTCACAGATGCAGCTGGAATCTAATGATGT20.17467248908296942No Hit
CGTGGACACCAGGGCTCGAGGGACAGCAGAGCAGAGCAGGGAACCTGCCC20.17467248908296942No Hit
GTACACACCTAGGCTGAAAAAGGACTGCAAAGTATCCAAACTTTCATTCA20.17467248908296942No Hit
TCTTTCTATTCCCAGTACAGTCTTTGTTGTTGAGTGCAACCATTCTGGTG20.17467248908296942No Hit
GAAGAAAACGTCCTCTGTGATGCACAGCCTCCTCCAGCCAGTGCCCTCTG20.17467248908296942No Hit
GCTCCCATGTGGCTTCTGTGGCTCTGGGCTCATTCGAACATGCTCCCTTT20.17467248908296942No Hit
CCCATCCACAGCTGCCACTGCAGTCTCTTTGCTGGAGACTCTCCGTCCTC20.17467248908296942No Hit
GTCGGTGCGGGGGCCGGCGGCATCGACGGCGTACGACGTCGCGAGCGTCA20.17467248908296942No Hit
CCCGAGCTCCAGGACATCTCCACTGACTGGCATTTCCTCACAGATGCAGC20.17467248908296942No Hit
GCAAAGAGACTGCAGTGGCAGCTGTGGATGGGGGTGAAATCACACCAGGG20.17467248908296942No Hit
ACTGAAAGGGAGCATGTTCGAATGAGCCCAGAGCCACAGAAGCCACATGG20.17467248908296942No Hit
CTTCTGGTGCCATCGTACCCCCTGGTCACTGGAGGTCTCTCGCCCCTAGC20.17467248908296942No Hit
GCTGGAGACTCTCCGTCCTCTGCCCCAGTCCTGACCTCTCTCCCGAGCTC20.17467248908296942No Hit
TATCAACGCAGAGTACATGGGGAGCACATTTGGATGCAGAAAGACGACGA20.17467248908296942No Hit
GTGCAGACCCATCTCTCGGCGGTTGCTGTGAGCAGAGCAGAAGGGGTAGC20.17467248908296942No Hit
CGTACCCCCTGGTCACTGGAGGTCTCTCGCCCCTAGCTCCACAGGCGTGC20.17467248908296942No Hit
CCTCAGGGGCTAGGACCAGAGGTGGAATGTTCAGAAGCACGGAGGGAGGA20.17467248908296942No Hit
TTATTACATTGTTTTGACCTGTCAGTTCAAGTAAGGCTGCATTTTAGAGC20.17467248908296942No Hit
GTGATGCACAGCCTCCTCCAGCCAGTGCCCTCTGCTACCCCTTCTGCTCT20.17467248908296942No Hit
ATCAGGAAGAATCAATGATGGATTCTGGGCTTAATACTTAAGTGATGGGA20.17467248908296942No Hit
GAGAGAAGACAAGCAAGACACGACCTCGTGGACACCAGGGCTCGAGGGAC20.17467248908296942No Hit
CACCCCCTCCCCACAGGCCTCTCGTTCCTCATTCTGTGGTCGTCCAGCTT20.17467248908296942No Hit
GGTTTACAGGTCCATTCCCTCGTTTCTTATGCCAAAACAATAAACTACAG20.17467248908296942No Hit
ATTAGATTCCAGCTGCATCTGTGAGGAAATGCCAGTCAGTGGAGATGTCC20.17467248908296942No Hit
CCTCTGCCCCAGTCCTGACCTCTCTCCCGAGCTCCAGGACATCTCCACTG20.17467248908296942No Hit
GTGTAGCTTTTGGCAGAAGAAGATTGGATTTTTGGGTCACGTGGTTTCAG20.17467248908296942No Hit
GCTCTGCTCACAGCAACCGCCGAGAGATGGGTCTGCACTTGCTGTCACCC20.17467248908296942No Hit
GGGTACGATGGCACCAGAAGCTGGACGACCACAGAATGAGGAACGAGAGG20.17467248908296942No Hit
CCTGTGGAGCTAGGGGCGAGAGACCTCCAGTGACCAGGGGGTACGATGGC20.17467248908296942No Hit
TCATTAGATTCCAGCTGCATCTGTGAGGAAATGCCAGTCAGTGGAGATGT20.17467248908296942No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG20.17467248908296942TruSeq Adapter, Index 9 (95% over 24bp)
GGACATTTGGAGGGATTTGAGGGCAATGGTGGAAAAGGAAATGTGTTCAC20.17467248908296942No Hit
GGCTGGAGGAGGCTGTGCATCACAGAGGACGTTTTCTTCAGTTAGATTGG20.17467248908296942No Hit
GCTCTGCTGTCCCTCGAGCCCTGGTGTCCACGAGGTCGTGTCTTGCTTGT20.17467248908296942No Hit
GTCCCATGGTCAAGTATATACAATTTTTCCTAGACAGAATCTATGGCTTT20.17467248908296942No Hit
CCAGGGGAATGTGCAGGGAGAGAAGACAAGCAAGACACGACCTCGTGGAC20.17467248908296942No Hit
GAGAAGACAAGCAAGACACGACCTCGTGGACACCAGGGCTCGAGGGACAG20.17467248908296942No Hit
GAGTACATGGGGAGCACATTTGGATGCAGAAAGACGACGACATCATTAGC20.17467248908296942No Hit
GAGCAGAAGGGGTAGCAGAGGGCACTGGCTGGAGGAGGCTGTGCATCACA20.17467248908296942No Hit
TCCCAATCTAACTGAAGAAAACGTCCTCTGTGATGCACAGCCTCCTCCAG20.17467248908296942No Hit
CCTCTTTGTCCCCTCTGTCCCTGGCTCGGGGCAGGTTCCCTGCTCTGCTC20.17467248908296942No Hit
CTCGTGGACACCAGGGCTCGAGGGACAGCAGAGCAGAGCAGGGAACCTGC20.17467248908296942No Hit
GCACTCTCTTGAACGTGCCTTGAGTCGTAAGGATCAGTCCCTGTCTCTTA20.17467248908296942No Hit
CCTCTGTGATGCACAGCCTCCTCCAGCCAGTGCCCTCTGCTACCCCTTCT20.17467248908296942No Hit
CAACTGAAAATTTCCTTCCATTTTAGAAGTTTTAGCTTGGTCCTACATCT20.17467248908296942No Hit
GGAAAGGAGGCAGCATTGTGCTGGTTCAAGATCTGATTTAAAGGCATTCC20.17467248908296942No Hit
GTTACAGTTCATATATGGAGAAACCGTTAGGCTGAACTCTGTCTCTTATA20.17467248908296942No Hit
CTAATATCCTGCAGTTGTTGGGAAGAAAAAGATGAAGATCAGGCCTGGAA20.17467248908296942No Hit
GCACTACACTCTCCTGGGCCACCTCCTCTTTGTCCCCTCTGTCCCTGGCT20.17467248908296942No Hit
GGGGCAGAGGACGGAGAGTCTCCAGCAAAGAGACTGCAGTGGCAGCTGTG20.17467248908296942No Hit
CCCTTTCAGTTCCCCCGGCTCTGTGGCACTACACTCTCCTGGGCCACCTC20.17467248908296942No Hit
CTGTGGCACTACACTCTCCTGGGCCACCTCCTCTTTGTCCCCTCTGTCCC20.17467248908296942No Hit
AGAGAGTACCCATTGGCAGGCAATGGCATATAAGATTAGCCAGGTAAAGT20.17467248908296942No Hit
CTTTACAAAAAAAGCTGTATATTTTTCATCTCACTGGCAACTGTCTCTTA20.17467248908296942No Hit
CCTGTGGGGAGGGGGTGACAGCAAGTGCAGACCCATCTCTCGGCGGTTGC20.17467248908296942No Hit
CTCCCCACAGGCCTCTCGTTCCTCATTCTGTGGTCGTCCAGCTTCTGGTG20.17467248908296942No Hit
CCCCGAGCCAGGGACAGAGGGGACAAAGAGGAGGTGGCCCAGGAGAGTGT20.17467248908296942No Hit
TTGGATAGCTTTGAGGATTTCGTTGGAAACGGGATTGTCTTCATATAAAC20.17467248908296942No Hit
GTATGGGTGCTGAGCCCAGACATGGACATTTTCTTAAAACTCTCCAGGTG20.17467248908296942No Hit
CTGTTGCGGTGTTTATGGGAAATCAATTTCCCGCCAGAAGCGTCGTCGCT20.17467248908296942No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.17467248908296942No Hit
CTCGACGCGCGAACGCGATCCGCGCAAGGGCCGCAACCCGCGCACCGGCG20.17467248908296942No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers