FastQCFastQC Report
Thu 2 Feb 2017
SRR4063897_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063897_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495955
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38190.2552884277936168No Hit
TATCAACGCAGAGTACTTTTTTTTT27150.1814894164597197No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21460.14345351297331804No Hit
CTATTGGAGCTGGAATTACCGCGGC17070.114107710459205No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16460.11003004769528495No Hit
GTACATGGAAGCAGTGGTATCAACG16140.10789094591749082No Hit
GAATAGGACCGCGGTTCTATTTTGT16130.10782409898693476No Hit
GTATCTGATCGTCTTCGAACCTCCG15970.10675454809803771No Hit
GTACATGGGGTGGTATCAACGCAAA15240.10187472216744488No Hit
GATTAAGAGGGACGGCCGGGGGCAT15070.10073832434799176No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14990.10020354890354323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT456.7804614E-414.7724981
TCTCGAA400.00527743614.250627512
ACACGTG400.00528212314.2487215
GTTTTAG954.9240043E-913.9949981
CGACCAT2650.013.6232410
CGAACGA1205.0931703E-1113.45937516
CTTAAAC852.7061287E-713.4087673
CCGACCA2750.013.1287299
AACCGCG1600.013.0626387
GCGTTAT1352.7284841E-1112.6621421
ACGAACG1301.9826984E-1012.42403915
CGTCGTA1950.012.18002310
AATGGTA550.003065802912.0922518
TTTCGTT1501.4551915E-1112.03466917
TTCGTTC1603.6379788E-1211.87750918
AGAACCG2200.011.6580455
GTTCTAG907.4896507E-611.6069631
ATAACGA1406.7848305E-1011.53622212
TAGCGTA1657.2759576E-1211.5152737
CAAGACT1657.2759576E-1211.5133484
Produced by FastQC (version 0.11.5)