##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4063897_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1495955 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.05791684910308 32.0 32.0 32.0 32.0 32.0 2 30.509498614597366 32.0 32.0 32.0 32.0 32.0 3 30.533973281281856 32.0 32.0 32.0 32.0 32.0 4 30.513778155091565 32.0 32.0 32.0 27.0 32.0 5 30.45211186165359 32.0 32.0 32.0 21.0 32.0 6 33.85785468145767 36.0 36.0 36.0 32.0 36.0 7 33.8095444047448 36.0 36.0 36.0 32.0 36.0 8 33.75268641102172 36.0 36.0 36.0 27.0 36.0 9 33.98636456310518 36.0 36.0 36.0 32.0 36.0 10 33.49094123820569 36.0 36.0 36.0 21.0 36.0 11 33.99644040094789 36.0 36.0 36.0 32.0 36.0 12 33.70155051455425 36.0 36.0 36.0 32.0 36.0 13 33.868214618755246 36.0 36.0 36.0 32.0 36.0 14 33.679190216283246 36.0 36.0 36.0 32.0 36.0 15 33.58346206938043 36.0 36.0 36.0 27.0 36.0 16 33.60564388634685 36.0 36.0 36.0 27.0 36.0 17 33.50570906210414 36.0 36.0 36.0 21.0 36.0 18 33.50629664662373 36.0 36.0 36.0 21.0 36.0 19 33.50666898402692 36.0 36.0 36.0 27.0 36.0 20 33.4670073631894 36.0 36.0 36.0 21.0 36.0 21 33.42022320190112 36.0 36.0 36.0 21.0 36.0 22 33.384995537967384 36.0 36.0 36.0 21.0 36.0 23 33.340454759668575 36.0 36.0 36.0 21.0 36.0 24 33.29342259626794 36.0 36.0 36.0 21.0 36.0 25 32.53186091827629 36.0 32.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 11.0 5 18.0 6 59.0 7 19.0 8 76.0 9 114.0 10 128.0 11 50.0 12 70.0 13 73.0 14 337.0 15 759.0 16 1182.0 17 1491.0 18 1795.0 19 2326.0 20 2971.0 21 3938.0 22 5315.0 23 7349.0 24 9772.0 25 13672.0 26 19208.0 27 26008.0 28 35862.0 29 50245.0 30 69753.0 31 102519.0 32 153690.0 33 220890.0 34 389421.0 35 376830.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.15494817005278 17.258793971529474 11.730132522297142 25.8561253361206 2 16.466360134610266 19.78602475876979 37.64046971870626 26.10714538791369 3 18.750718615552046 24.553455638357892 28.010898891126068 28.684926854963994 4 12.51187406703759 15.65565239015527 35.45394198970925 36.378531553097886 5 14.698536470575652 36.56102674357349 33.238469992699734 15.50196679315112 6 34.08484960314914 35.12096731061335 17.217618146032564 13.57656494020495 7 30.216761942967942 30.001872095744325 21.054925951927256 18.72644000936048 8 28.53442142371866 32.50924127896091 19.21677820395543 19.739559093365003 9 27.697610543063256 14.239702744040066 18.248190803443105 39.81449590945357 10 16.025611013832837 26.690646615897663 31.163076585572703 26.120665784696794 11 37.1885907974898 20.835231099420206 22.486471519463556 19.489706583626443 12 25.371959311223126 23.169098682591155 28.764541261330677 22.69440074485504 13 29.93414143738353 19.394965425257958 25.02331069375568 25.647582443602836 14 23.902013863665704 19.637562065757095 25.05954901280078 31.400875057776425 15 25.139538134626793 27.20935101139648 22.313125915065836 25.33798493891089 16 25.62883984441628 25.34549820570362 24.123905111319953 24.90175683856015 17 24.014974619798632 25.667948440968708 25.25124359307935 25.065833346153305 18 24.880337323315675 24.57120456079533 26.521129200904188 24.02732891498481 19 25.93235282706101 24.637193502596205 24.90450700192792 24.525946668414864 20 25.936691382867416 24.035546374317985 24.920191914029367 25.107570328785233 21 26.930502188781023 23.980865558540525 24.121477346919118 24.96715490575933 22 25.861984767549593 24.30843537915556 24.556355718189632 25.27322413510521 23 24.34957244715966 24.16713830608529 25.402766683659067 26.080522563095986 24 24.969613372390544 24.972623605175194 24.9285404183956 25.129222604038663 25 24.982406002563508 24.495861731249345 25.040138397193523 25.481593868993624 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 147.0 1 147.0 2 417.0 3 687.0 4 687.0 5 687.0 6 1763.5 7 2840.0 8 2840.0 9 2840.0 10 2777.0 11 2714.0 12 2714.0 13 2714.0 14 2875.5 15 3037.0 16 3037.0 17 3037.0 18 5488.0 19 7939.0 20 7939.0 21 7939.0 22 13710.5 23 19482.0 24 19482.0 25 19482.0 26 30584.0 27 41686.0 28 41686.0 29 41686.0 30 53071.5 31 64457.0 32 64457.0 33 64457.0 34 78750.5 35 93044.0 36 93044.0 37 93044.0 38 105760.5 39 118477.0 40 118477.0 41 118477.0 42 136467.5 43 154458.0 44 154458.0 45 154458.0 46 176146.5 47 197835.0 48 197835.0 49 197835.0 50 199546.0 51 201257.0 52 201257.0 53 201257.0 54 188233.0 55 175209.0 56 175209.0 57 175209.0 58 162337.5 59 149466.0 60 149466.0 61 149466.0 62 131385.5 63 113305.0 64 113305.0 65 113305.0 66 93399.5 67 73494.0 68 73494.0 69 73494.0 70 54995.0 71 36496.0 72 36496.0 73 36496.0 74 27922.5 75 19349.0 76 19349.0 77 19349.0 78 15803.5 79 12258.0 80 12258.0 81 12258.0 82 8271.5 83 4285.0 84 4285.0 85 4285.0 86 3266.5 87 2248.0 88 2248.0 89 2248.0 90 1546.5 91 845.0 92 845.0 93 845.0 94 531.0 95 217.0 96 217.0 97 217.0 98 470.0 99 723.0 100 723.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039038607444742655 2 0.0054146013750413615 3 0.0015374794027895225 4 0.0032086526666911773 5 0.007954784736171876 6 0.011965600569535849 7 0.020388313819600188 8 0.0328218429030285 9 0.04532221891701287 10 0.05461394226430608 11 0.051873218111507365 12 0.060095390569903505 13 0.06236818620880976 14 0.06470782877827208 15 0.05641880938931986 16 0.06283611472270222 17 0.05962746205601104 18 0.07540333766724266 19 0.07226153193110756 20 0.07480171529223807 21 0.0707908994588741 22 0.07486856222279414 23 0.07814406182004138 24 0.07045666480609378 25 0.07533649073668659 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1495955.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.20037691819637 #Duplication Level Percentage of deduplicated Percentage of total 1 69.5134457455511 31.420339485815035 2 16.08461470534424 14.540612945310475 3 6.198083257014406 8.404670981622399 4 2.84916496249743 5.151333208280107 5 1.5847504963250314 3.58156598775951 6 0.9595316024528351 2.6022714057473464 7 0.5851844312500692 1.8515389801414448 8 0.3961427316312582 1.4324640626509426 9 0.2958288109820213 1.203441638368431 >10 1.320375118641288 10.627307153487463 >50 0.10262665684200058 3.2484920685420726 >100 0.09323286436519054 9.07092618961184 >500 0.012283001909155019 3.7066377659566663 >1k 0.004735615193891091 3.1583981267062944 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3819 0.2552884277936168 No Hit TATCAACGCAGAGTACTTTTTTTTT 2715 0.1814894164597197 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2146 0.14345351297331804 No Hit CTATTGGAGCTGGAATTACCGCGGC 1707 0.114107710459205 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 1646 0.11003004769528495 No Hit GTACATGGAAGCAGTGGTATCAACG 1614 0.10789094591749082 No Hit GAATAGGACCGCGGTTCTATTTTGT 1613 0.10782409898693476 No Hit GTATCTGATCGTCTTCGAACCTCCG 1597 0.10675454809803771 No Hit GTACATGGGGTGGTATCAACGCAAA 1524 0.10187472216744488 No Hit GATTAAGAGGGACGGCCGGGGGCAT 1507 0.10073832434799176 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1499 0.10020354890354323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.3423465278033097E-4 2 0.0 0.0 0.0 0.0 3.3423465278033097E-4 3 0.0 0.0 0.0 0.0 3.3423465278033097E-4 4 0.0 0.0 0.0 0.0 3.3423465278033097E-4 5 0.0 0.0 0.0 0.0 3.3423465278033097E-4 6 0.0 0.0 0.0 0.0 4.6792851389246336E-4 7 0.0 0.0 0.0 0.0 4.6792851389246336E-4 8 0.0 0.0 0.0 0.0 4.6792851389246336E-4 9 0.0 0.0 0.0 0.0 4.6792851389246336E-4 10 0.0 0.0 0.0 6.68469305560662E-5 6.016223750045957E-4 11 0.0 0.0 0.0 6.68469305560662E-5 6.016223750045957E-4 12 0.0 0.0 0.0 6.68469305560662E-5 0.0012700916805652577 13 0.0 0.0 0.0 6.68469305560662E-5 0.0016711732639016548 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAAT 45 6.7804614E-4 14.772498 1 TCTCGAA 40 0.005277436 14.2506275 12 ACACGTG 40 0.005282123 14.248721 5 GTTTTAG 95 4.9240043E-9 13.994998 1 CGACCAT 265 0.0 13.62324 10 CGAACGA 120 5.0931703E-11 13.459375 16 CTTAAAC 85 2.7061287E-7 13.408767 3 CCGACCA 275 0.0 13.128729 9 AACCGCG 160 0.0 13.062638 7 GCGTTAT 135 2.7284841E-11 12.662142 1 ACGAACG 130 1.9826984E-10 12.424039 15 CGTCGTA 195 0.0 12.180023 10 AATGGTA 55 0.0030658029 12.092251 8 TTTCGTT 150 1.4551915E-11 12.034669 17 TTCGTTC 160 3.6379788E-12 11.877509 18 AGAACCG 220 0.0 11.658045 5 GTTCTAG 90 7.4896507E-6 11.606963 1 ATAACGA 140 6.7848305E-10 11.536222 12 TAGCGTA 165 7.2759576E-12 11.515273 7 CAAGACT 165 7.2759576E-12 11.513348 4 >>END_MODULE