FastQCFastQC Report
Thu 2 Feb 2017
SRR4063897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495955
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16570.11076536393140168No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15330.10247634454244947No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA15110.10100571207021602No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14970.10006985504243109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA602.8733662E-421.99983412
TATATGA1101.2666897E-719.999182
GATATAC2700.019.5573671
TCCCCGC4550.018.3753281
ATAGACT1509.606083E-917.5992774
CGTCTTA1408.376628E-817.28558315
CGCCCCT4700.016.8503725
ATAGCGT4100.016.6334656
GTAGGAC6750.016.621543
GATTTCG3050.016.59003641
GCATACG1206.40904E-616.49987637
CTAGACC800.001989192816.4993233
AATAGCG4300.016.371425
CTAGAAC1753.6288839E-916.3421883
GAATTAG953.344615E-416.2120291
GTATCAA43300.016.1077291
TAGCGTA4050.015.75243957
CCAATAG4550.015.4718913
CGTCGTA4000.015.39988310
TACGCTA5300.015.3583759
Produced by FastQC (version 0.11.5)