##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063897_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1495955
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00003141805736	32.0	32.0	32.0	32.0	32.0
2	30.741901327245806	32.0	32.0	32.0	32.0	32.0
3	31.113767459582675	32.0	32.0	32.0	32.0	32.0
4	31.329061368824597	32.0	32.0	32.0	32.0	32.0
5	31.14198354897039	32.0	32.0	32.0	32.0	32.0
6	34.76054827852442	36.0	36.0	36.0	32.0	36.0
7	34.801160462714456	36.0	36.0	36.0	32.0	36.0
8	34.71249736790211	36.0	36.0	36.0	32.0	36.0
9	34.9315741449442	36.0	36.0	36.0	32.0	36.0
10	34.63226835031802	36.0	36.0	36.0	32.0	36.0
11	34.95105601438546	36.0	36.0	36.0	36.0	36.0
12	34.7812701585275	36.0	36.0	36.0	32.0	36.0
13	34.873668659819316	36.0	36.0	36.0	32.0	36.0
14	34.79304257146772	36.0	36.0	36.0	32.0	36.0
15	34.71843270686618	36.0	36.0	36.0	32.0	36.0
16	34.74982803627115	36.0	36.0	36.0	32.0	36.0
17	34.69643204508157	36.0	36.0	36.0	32.0	36.0
18	34.69397007262919	36.0	36.0	36.0	32.0	36.0
19	34.707193063962485	36.0	36.0	36.0	32.0	36.0
20	34.69228085069404	36.0	36.0	36.0	32.0	36.0
21	34.685900979641765	36.0	36.0	36.0	32.0	36.0
22	34.66760230087135	36.0	36.0	36.0	32.0	36.0
23	34.59528461751857	36.0	36.0	36.0	32.0	36.0
24	34.56636864076794	36.0	36.0	36.0	32.0	36.0
25	34.52463677049109	36.0	36.0	36.0	32.0	36.0
26	34.4501532465883	36.0	36.0	36.0	32.0	36.0
27	34.43923914823641	36.0	36.0	36.0	32.0	36.0
28	34.404051592461	36.0	36.0	36.0	32.0	36.0
29	34.36725102025128	36.0	36.0	36.0	32.0	36.0
30	34.3440644939186	36.0	36.0	36.0	32.0	36.0
31	34.33917865176426	36.0	36.0	36.0	32.0	36.0
32	34.28421376311453	36.0	36.0	36.0	32.0	36.0
33	34.25018199076844	36.0	36.0	36.0	32.0	36.0
34	34.229824426536894	36.0	36.0	36.0	32.0	36.0
35	34.186308411683505	36.0	36.0	36.0	32.0	36.0
36	34.13543254977589	36.0	36.0	36.0	32.0	36.0
37	34.12592290543499	36.0	36.0	36.0	32.0	36.0
38	34.05966088552129	36.0	36.0	36.0	32.0	36.0
39	34.04756092262134	36.0	36.0	36.0	32.0	36.0
40	34.018281298568475	36.0	36.0	36.0	32.0	36.0
41	34.003293548268495	36.0	36.0	36.0	32.0	36.0
42	33.89768475656019	36.0	36.0	36.0	32.0	36.0
43	33.91532365612602	36.0	36.0	36.0	32.0	36.0
44	33.81938494139195	36.0	36.0	36.0	32.0	36.0
45	33.76518210775057	36.0	36.0	36.0	32.0	36.0
46	33.743287732585536	36.0	36.0	36.0	32.0	36.0
47	33.70440086767316	36.0	36.0	36.0	27.0	36.0
48	33.65511395730486	36.0	36.0	36.0	27.0	36.0
49	33.6334789482304	36.0	36.0	36.0	27.0	36.0
50	32.87492203976724	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	4.0
20	12.0
21	57.0
22	171.0
23	479.0
24	1336.0
25	3157.0
26	6379.0
27	12182.0
28	20760.0
29	32563.0
30	49761.0
31	75068.0
32	114209.0
33	190747.0
34	360634.0
35	628431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.70218993131646	18.52998236449474	12.590199793294229	26.177627910894568
2	15.843711353989104	20.710852635449044	36.649754336709115	26.795681673852734
3	18.297086675125037	25.17403610004051	28.340777858142697	28.188099366691755
4	12.472099762359163	16.568680207626567	35.73623538141187	35.2229846486024
5	14.910074166669451	36.62075396652974	32.51682035890117	15.952351507899637
6	34.43822692053416	35.66453714058816	16.577748870116736	13.31948706876094
7	30.200681438521134	30.15315320735344	20.88434596541469	18.76181938871074
8	28.58194263864889	32.66014017801338	18.928644243977928	19.829272939359807
9	27.745902762597872	13.927164531898523	17.876435461951527	40.45049724355208
10	15.874637186101637	26.82155639307836	31.077726067652172	26.22608035316783
11	37.65902049192656	20.647479369366057	22.25013452944774	19.443365609259637
12	25.37760210380941	23.151061564099944	28.612596786128975	22.85873954596167
13	30.092830580680786	19.363201067414643	24.919933874839483	25.624034477065088
14	24.074419450343694	19.387173260122477	24.826181036436303	31.712226253097526
15	25.373490528702686	27.052894334335193	22.154532959686822	25.419082177275303
16	25.848964530662993	25.284102303568325	23.86198644330655	25.00494672246213
17	24.182946679545843	25.614473697404	24.992797243232584	25.209782379817575
18	25.27905344429961	24.476620207894648	26.29573180921822	23.94859453858752
19	26.07245944728247	24.40101767835107	25.03225366555389	24.494269208812568
20	26.181214608349674	23.79963354120723	24.921203337836637	25.09794851260646
21	27.109237911568197	23.841225170543233	24.226062949754503	24.823473968134067
22	26.1179328494946	24.100890870234558	24.60224351968277	25.17893276058807
23	24.565007403347046	23.908231920291186	25.467313303630817	26.059447372730947
24	25.271950752397636	24.749406899022762	24.90582913477781	25.072813213801787
25	25.353926988562296	24.16550239650251	25.12731210684992	25.35325850808527
26	24.748141898893095	25.063206857225367	25.427740958177182	24.760910285704355
27	25.50788407761941	24.61983483781603	24.61716079614243	25.255120288422134
28	24.62667809350128	24.631090170827612	25.313825712249667	25.428406023421445
29	24.899610474603307	24.318363979964207	25.35197333017809	25.430052215254396
30	24.665117541436647	24.65148024798482	25.6849731733046	24.99842903727393
31	25.406045744533635	24.459480328299556	24.582555893755234	25.551918033411575
32	24.99473560714995	24.7260677358565	24.47110399833144	25.80809265866211
33	24.52828927357384	24.34879686606925	25.266848721659752	25.85606513869716
34	25.255010124197735	24.4641929620615	25.38763744876902	24.893159464971745
35	25.8699536670916	24.275943855023655	25.28114937165464	24.57295310623011
36	24.89530937883565	25.013571006447233	24.746897387146717	25.344222227570402
37	25.589781094114954	24.785512877249325	24.576340302187187	25.04836572644853
38	24.990323052928055	24.649507328072907	24.952083234446857	25.40808638455218
39	25.547850745809686	24.19019835001347	24.708618475544007	25.553332428632835
40	25.932425124221965	24.62967382417804	24.91812681369413	24.519774237905864
41	24.80222634905228	24.81566305814151	25.421651953135964	24.960458639670247
42	26.124286896544348	25.287213404941156	24.651018833819318	23.93748086469518
43	25.15426744771351	24.328165348767097	25.026254377679336	25.49131282584006
44	24.94560258299993	24.80883193454261	24.7005384592245	25.545027023232965
45	25.180038384123332	25.089325644030374	24.559220980112052	25.171414991734242
46	24.874961395458904	24.760584997506584	24.84113646421175	25.523317142822766
47	25.496614810338464	24.457594215764395	25.05441438357996	24.99137659031718
48	25.809998876951294	25.336780914589475	24.002291554138967	24.850928654320263
49	25.144524126571582	25.147866497676386	24.48915200034226	25.218457375409777
50	25.360077008950952	25.382537952979085	23.97318056326165	25.284204474808313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	22.0
2	22.0
3	78.0
4	134.0
5	161.5
6	189.0
7	188.5
8	188.0
9	261.0
10	334.0
11	534.5
12	735.0
13	1272.0
14	1809.0
15	2679.0
16	3549.0
17	3973.0
18	4397.0
19	4635.0
20	4873.0
21	5284.0
22	5695.0
23	6351.0
24	7007.0
25	7458.5
26	7910.0
27	10215.0
28	12520.0
29	14635.0
30	16750.0
31	19965.0
32	23180.0
33	27507.0
34	31834.0
35	36987.5
36	42141.0
37	48021.5
38	53902.0
39	57866.5
40	61831.0
41	67295.5
42	72760.0
43	74390.5
44	76021.0
45	84420.0
46	92819.0
47	100685.5
48	108552.0
49	112077.0
50	115602.0
51	111221.5
52	106841.0
53	106367.5
54	105894.0
55	107761.0
56	109628.0
57	107694.5
58	105761.0
59	97117.5
60	88474.0
61	78946.0
62	69418.0
63	59733.0
64	50048.0
65	42657.5
66	35267.0
67	30460.0
68	25653.0
69	23142.5
70	20632.0
71	16161.0
72	11690.0
73	9857.0
74	8024.0
75	6397.5
76	4771.0
77	4112.5
78	3454.0
79	2874.5
80	2295.0
81	1846.0
82	1397.0
83	1156.0
84	915.0
85	672.0
86	429.0
87	326.0
88	223.0
89	174.0
90	125.0
91	102.0
92	79.0
93	68.5
94	58.0
95	49.5
96	41.0
97	35.5
98	30.0
99	31.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007286315430611216
2	3.3423465278033097E-4
3	6.68469305560662E-5
4	0.0
5	0.0
6	2.673877222242648E-4
7	1.336938611121324E-4
8	0.0
9	5.347754444485295E-4
10	6.016223750045957E-4
11	0.0
12	1.336938611121324E-4
13	2.673877222242648E-4
14	1.336938611121324E-4
15	0.004478744347256434
16	0.001002703958340993
17	0.0
18	3.3423465278033097E-4
19	6.68469305560662E-5
20	0.001069550888897059
21	0.0
22	1.336938611121324E-4
23	6.684693055606619E-4
24	2.673877222242648E-4
25	0.0016711732639016548
26	0.004879825930592832
27	0.00614991761115809
28	0.004077662763920038
29	0.002139101777794118
30	0.003810275041695773
31	0.008422713250064339
32	0.003743428111139707
33	0.004679285138924634
34	0.0022727956389062506
35	0.00280757108335478
36	0.007954784736171876
37	0.004077662763920038
38	0.008823794833400737
39	0.004278203555588236
40	0.0029412649444669125
41	0.003542887319471508
42	0.0019385609861259194
43	9.358570277849267E-4
44	0.0013369386111213239
45	0.0016043263333455886
46	0.0011363978194531253
47	0.0018048671250137872
48	0.0018048671250137872
49	7.353162361167281E-4
50	0.0016711732639016548
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1495955.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.39627160559689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.56676521693176	36.57094409412073
2	14.65760970793511	14.773777598598627
3	5.619110120102081	8.495465993832678
4	2.584161244336232	5.209283677689039
5	1.383069423196425	3.4850771150401623
6	0.8440333244133147	2.5521679596784965
7	0.5013620443436464	1.7686744431635801
8	0.3535965484445419	1.4255958155370185
9	0.24014253058938145	1.0892059376074044
>10	1.0859925600417282	9.533340727764866
>50	0.07880845999627963	2.7675473196965736
>100	0.07363177089739703	7.633808499465091
>500	0.008920934860668089	2.9319447202115567
>1k	0.0027961139115526847	1.7631660975941514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1657	0.11076536393140168	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1533	0.10247634454244947	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1511	0.10100571207021602	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1497	0.10006985504243109	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.347754444485295E-4
2	0.0	0.0	0.0	0.0	5.347754444485295E-4
3	0.0	0.0	0.0	0.0	5.347754444485295E-4
4	0.0	0.0	0.0	0.0	5.347754444485295E-4
5	0.0	0.0	0.0	0.0	5.347754444485295E-4
6	0.0	0.0	0.0	0.0	7.353162361167281E-4
7	0.0	0.0	0.0	0.0	7.353162361167281E-4
8	0.0	0.0	0.0	0.0	7.353162361167281E-4
9	0.0	0.0	0.0	0.0	7.353162361167281E-4
10	0.0	0.0	0.0	6.68469305560662E-5	7.353162361167281E-4
11	0.0	0.0	0.0	6.68469305560662E-5	7.353162361167281E-4
12	0.0	0.0	0.0	6.68469305560662E-5	0.0012032447500091915
13	0.0	0.0	0.0	6.68469305560662E-5	0.0016711732639016548
14	0.0	0.0	0.0	6.68469305560662E-5	0.001738020194457721
15	0.0	0.0	0.0	6.68469305560662E-5	0.002005407916681986
16	0.0	0.0	0.0	6.68469305560662E-5	0.002139101777794118
17	0.0	0.0	0.0	6.68469305560662E-5	0.0022727956389062506
18	0.0	0.0	0.0	2.0054079166819858E-4	0.002473336430574449
19	0.0	0.0	0.0	2.0054079166819858E-4	0.002473336430574449
20	0.0	0.0	0.0	2.0054079166819858E-4	0.00280757108335478
21	0.0	0.0	0.0	4.0108158333639716E-4	0.0029412649444669125
22	0.0	0.0	0.0	8.690100972288605E-4	0.003074958805579045
23	0.0	0.0	0.0	0.0018717140555698534	0.003074958805579045
24	0.0	0.0	0.0	0.0032754995972472433	0.003074958805579045
25	0.0	0.0	0.0	0.0038771219722518392	0.003074958805579045
26	0.0	0.0	0.0	0.004679285138924633	0.003476040388915442
27	0.0	0.0	0.0	0.0054814483055974274	0.0036765811805836404
28	0.0	0.0	0.0	0.007420009291723348	0.0036765811805836404
29	0.0	0.0	0.0	0.010829202750082723	0.0036765811805836404
30	0.0	0.0	0.0	0.01804867125013787	0.0036765811805836404
31	0.0	0.0	0.0	0.03161859815301931	0.003743428111139707
32	0.0	0.0	0.0	0.04431951495867188	0.003743428111139707
33	0.0	0.0	0.0	0.059226380472674645	0.003943968902807906
34	0.0	0.0	0.0	0.07379901133389707	0.004478744347256435
35	0.0	0.0	0.0	0.09351885584793661	0.004478744347256435
36	0.0	0.0	0.0	0.11865330173701749	0.004478744347256435
37	0.0	0.0	0.0	0.15615442977897062	0.004478744347256435
38	0.0	0.0	0.0	0.2016771894876517	0.005013519791704965
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGCTA	60	2.8733662E-4	21.999834	12
TATATGA	110	1.2666897E-7	19.99918	2
GATATAC	270	0.0	19.557367	1
TCCCCGC	455	0.0	18.375328	1
ATAGACT	150	9.606083E-9	17.599277	4
CGTCTTA	140	8.376628E-8	17.285583	15
CGCCCCT	470	0.0	16.850372	5
ATAGCGT	410	0.0	16.633465	6
GTAGGAC	675	0.0	16.62154	3
GATTTCG	305	0.0	16.590036	41
GCATACG	120	6.40904E-6	16.499876	37
CTAGACC	80	0.0019891928	16.499323	3
AATAGCG	430	0.0	16.37142	5
CTAGAAC	175	3.6288839E-9	16.342188	3
GAATTAG	95	3.344615E-4	16.212029	1
GTATCAA	4330	0.0	16.107729	1
TAGCGTA	405	0.0	15.7524395	7
CCAATAG	455	0.0	15.471891	3
CGTCGTA	400	0.0	15.399883	10
TACGCTA	530	0.0	15.358375	9
>>END_MODULE