FastQCFastQC Report
Thu 2 Feb 2017
SRR4063896_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063896_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3656062
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54100.14797342058203608No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG44910.12283708536671425No Hit
CTATTGGAGCTGGAATTACCGCGGC43230.11824197729688392No Hit
GAATAGGACCGCGGTTCTATTTTGT43130.11796845895939402No Hit
GTATCTGATCGTCTTCGAACCTCCG40840.1117048890308753No Hit
GATTAAGAGGGACGGCCGGGGGCAT39900.10913381665847023No Hit
GTCTTGCGCCGGTCCAAGAATTTCA39630.1083953171472475No Hit
GTACATGGGGAATAATTGCAATCCC38070.10412843108240505No Hit
GTCCTATTCCATTATTCCTAGCTGC37980.10388226457866415No Hit
GAACTACGACGGTATCTGATCGTCT37240.10185822888123888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45800.012.4399561
CCGACCA7050.012.1281489
GCGTTAT2700.011.9577161
TCGCGTA3100.011.9520959
CGTCTTA3250.011.69486115
CGCGTAA3200.011.57890910
CGACCAT7350.011.2456610
CGTCGTA6350.011.07161610
CGAACGT2950.010.9465964
TCGAACG3000.010.7628283
GGTTCTA8500.010.61894713
GCGTAAC3500.010.58657611
GACCATA8000.010.56961111
CGGTTCT8450.010.56948812
GTCTAGT1355.7321813E-810.5509261
CCGTCGT6950.010.5256059
CGAACGA3650.010.41323316
CCTATTC9250.010.1639433
GCGCCGA4500.010.13526819
GGTCGCG4050.010.0865517
Produced by FastQC (version 0.11.5)