##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063896_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3656062
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.207951068663498	32.0	32.0	32.0	32.0	32.0
2	30.732911531587813	32.0	32.0	32.0	32.0	32.0
3	30.741257123101303	32.0	32.0	32.0	32.0	32.0
4	30.783016808795914	32.0	32.0	32.0	32.0	32.0
5	30.585355499988786	32.0	32.0	32.0	32.0	32.0
6	34.28812284912017	36.0	36.0	36.0	32.0	36.0
7	34.2254726533631	36.0	36.0	36.0	32.0	36.0
8	34.161874169529945	36.0	36.0	36.0	32.0	36.0
9	34.37512492950065	36.0	36.0	36.0	32.0	36.0
10	33.94546509331625	36.0	36.0	36.0	32.0	36.0
11	34.33598199374081	36.0	36.0	36.0	32.0	36.0
12	34.084854414394506	36.0	36.0	36.0	32.0	36.0
13	34.21692247013316	36.0	36.0	36.0	32.0	36.0
14	34.08416788336741	36.0	36.0	36.0	32.0	36.0
15	33.98309656674312	36.0	36.0	36.0	32.0	36.0
16	33.99266341763351	36.0	36.0	36.0	32.0	36.0
17	33.893545021938905	36.0	36.0	36.0	32.0	36.0
18	33.888096810174446	36.0	36.0	36.0	32.0	36.0
19	33.92687624006376	36.0	36.0	36.0	32.0	36.0
20	33.90641843601121	36.0	36.0	36.0	32.0	36.0
21	33.88686871283912	36.0	36.0	36.0	32.0	36.0
22	33.869561566516104	36.0	36.0	36.0	32.0	36.0
23	33.79211266110914	36.0	36.0	36.0	32.0	36.0
24	33.770930033462236	36.0	36.0	36.0	32.0	36.0
25	33.329521490609295	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	42.0
5	48.0
6	144.0
7	51.0
8	200.0
9	233.0
10	328.0
11	118.0
12	222.0
13	198.0
14	309.0
15	535.0
16	1026.0
17	1287.0
18	1709.0
19	2433.0
20	3777.0
21	5842.0
22	9197.0
23	14429.0
24	21723.0
25	31461.0
26	45678.0
27	60960.0
28	83203.0
29	113198.0
30	148646.0
31	202567.0
32	284683.0
33	392184.0
34	784873.0
35	1444747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21421441580274	17.097341035654246	11.538650970159708	26.149793578383303
2	17.124321668822574	19.334671281374742	36.7531518687735	26.787855181029187
3	18.708915083308376	23.584660263485045	27.976296466488606	29.73012818671798
4	12.777707571895839	15.305584321731857	35.291147439463835	36.62556066690847
5	15.272588411617653	36.10020808526271	32.87949573722007	15.747707765899566
6	34.86340851718965	34.811816044110195	16.747912501791784	13.576862936908368
7	31.14675028780789	29.810987914257325	20.562451028885846	18.479810769048942
8	28.9473410595048	31.805938769233798	19.027822316297303	20.218897854964098
9	27.570330409187893	14.25574947999432	18.218820798694793	39.955099312123
10	16.41893080902112	25.892572959904587	30.580802904466086	27.107693326608207
11	37.54691756875656	20.783850093664093	22.194298600306787	19.47493373727256
12	25.55381972764167	23.049082085197643	27.855585929503007	23.541512257657686
13	29.40100374165681	19.00392722065594	25.140151335799672	26.454917701887577
14	24.323674836223404	19.244417499976052	24.412983888515587	32.01892377528496
15	25.65406911107906	26.563245359629562	21.67263250860666	26.110053020684713
16	26.654196777371148	25.191393367803432	22.858653695354256	25.29575615947116
17	24.749484710171878	25.38138015840629	24.468405090743282	25.400730040678543
18	25.714635304010496	24.29064764585732	25.231149586314917	24.763567463817267
19	26.179590596614354	24.419897820228748	24.603728343595563	24.796783239561336
20	26.361881976798294	23.710520697036554	23.98491177549423	25.942685550670923
21	27.53028181273725	23.582533062823472	23.677596549794874	25.20958857464441
22	26.597996841151527	23.48642160499065	24.18555634329073	25.730025210567085
23	24.95801468521074	23.401293654119844	24.897325887891885	26.743365772777526
24	25.394081152509617	24.349639840744324	24.342687117891078	25.913591888854974
25	25.52156855016302	23.830448374998873	24.427641182845235	26.220341891992877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	152.0
2	458.5
3	765.0
4	765.0
5	765.0
6	2049.5
7	3334.0
8	3334.0
9	3334.0
10	3635.5
11	3937.0
12	3937.0
13	3937.0
14	5243.0
15	6549.0
16	6549.0
17	6549.0
18	11317.5
19	16086.0
20	16086.0
21	16086.0
22	28172.0
23	40258.0
24	40258.0
25	40258.0
26	62175.0
27	84092.0
28	84092.0
29	84092.0
30	106858.0
31	129624.0
32	129624.0
33	129624.0
34	169384.5
35	209145.0
36	209145.0
37	209145.0
38	245190.5
39	281236.0
40	281236.0
41	281236.0
42	328369.5
43	375503.0
44	375503.0
45	375503.0
46	424586.5
47	473670.0
48	473670.0
49	473670.0
50	488392.0
51	503114.0
52	503114.0
53	503114.0
54	474455.5
55	445797.0
56	445797.0
57	445797.0
58	413900.0
59	382003.0
60	382003.0
61	382003.0
62	338165.5
63	294328.0
64	294328.0
65	294328.0
66	243702.5
67	193077.0
68	193077.0
69	193077.0
70	146332.5
71	99588.0
72	99588.0
73	99588.0
74	76800.0
75	54012.0
76	54012.0
77	54012.0
78	43825.5
79	33639.0
80	33639.0
81	33639.0
82	23208.0
83	12777.0
84	12777.0
85	12777.0
86	9794.5
87	6812.0
88	6812.0
89	6812.0
90	4996.0
91	3180.0
92	3180.0
93	3180.0
94	2225.0
95	1270.0
96	1270.0
97	1270.0
98	1692.0
99	2114.0
100	2114.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03845667825107999
2	0.005880644256032857
3	0.0018325728611823322
4	0.004048071394850525
5	0.009545789978397522
6	0.013402398537005117
7	0.023139651351645567
8	0.03550268020618906
9	0.04854950490445731
10	0.057520906374126046
11	0.0559892036841826
12	0.06416740197513063
13	0.06381182813639374
14	0.06720345552126851
15	0.060310793416523026
16	0.06731286285626446
17	0.06271775478643415
18	0.0765851344971721
19	0.07376789562102612
20	0.07882798486458928
21	0.07423287679475896
22	0.07746039317713978
23	0.08202814941322112
24	0.07705011567090492
25	0.07666718999841907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3656062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.54085337067818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19022500125912	31.135746299455086
2	14.215413636916397	12.094716542631987
3	4.985230644641152	6.36227897618072
4	2.376194392613732	4.04341348945634
5	1.2390270139854318	2.635463326213174
6	0.8113514331802499	2.0709349410606364
7	0.5207818016255833	1.5508151582750085
8	0.402052346185283	1.3682919925124213
9	0.31107186086589783	1.1909936178756146
>10	1.7145662185757526	13.357546116826052
>50	0.12107782130295537	3.557366155158157
>100	0.08648921831799959	7.508147931707963
>500	0.015807930853239612	4.70584297564727
>1k	0.010646157510578414	8.269947280357423
>5k	6.452216673077826E-5	0.14849519664223962
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5410	0.14797342058203608	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	4491	0.12283708536671425	No Hit
CTATTGGAGCTGGAATTACCGCGGC	4323	0.11824197729688392	No Hit
GAATAGGACCGCGGTTCTATTTTGT	4313	0.11796845895939402	No Hit
GTATCTGATCGTCTTCGAACCTCCG	4084	0.1117048890308753	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3990	0.10913381665847023	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3963	0.1083953171472475	No Hit
GTACATGGGGAATAATTGCAATCCC	3807	0.10412843108240505	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3798	0.10388226457866415	No Hit
GAACTACGACGGTATCTGATCGTCT	3724	0.10185822888123888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9146283624293023E-4
2	0.0	0.0	0.0	0.0	2.1881466999192027E-4
3	0.0	0.0	0.0	0.0	2.1881466999192027E-4
4	0.0	0.0	0.0	0.0	2.1881466999192027E-4
5	0.0	0.0	0.0	2.7351833748990034E-5	2.461665037409103E-4
6	0.0	0.0	0.0	2.7351833748990034E-5	3.282220049878804E-4
7	0.0	0.0	0.0	2.7351833748990034E-5	3.5557383873687046E-4
8	0.0	0.0	0.0	2.7351833748990034E-5	3.5557383873687046E-4
9	0.0	0.0	0.0	2.7351833748990034E-5	3.8292567248586045E-4
10	0.0	0.0	0.0	2.7351833748990034E-5	3.8292567248586045E-4
11	0.0	0.0	0.0	2.7351833748990034E-5	3.8292567248586045E-4
12	0.0	0.0	0.0	2.7351833748990034E-5	7.111476774737409E-4
13	0.0	0.0	0.0	2.7351833748990034E-5	7.384995112227309E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4580	0.0	12.439956	1
CCGACCA	705	0.0	12.128148	9
GCGTTAT	270	0.0	11.957716	1
TCGCGTA	310	0.0	11.952095	9
CGTCTTA	325	0.0	11.694861	15
CGCGTAA	320	0.0	11.578909	10
CGACCAT	735	0.0	11.24566	10
CGTCGTA	635	0.0	11.071616	10
CGAACGT	295	0.0	10.946596	4
TCGAACG	300	0.0	10.762828	3
GGTTCTA	850	0.0	10.618947	13
GCGTAAC	350	0.0	10.586576	11
GACCATA	800	0.0	10.569611	11
CGGTTCT	845	0.0	10.569488	12
GTCTAGT	135	5.7321813E-8	10.550926	1
CCGTCGT	695	0.0	10.525605	9
CGAACGA	365	0.0	10.413233	16
CCTATTC	925	0.0	10.163943	3
GCGCCGA	450	0.0	10.135268	19
GGTCGCG	405	0.0	10.086551	7
>>END_MODULE