FastQCFastQC Report
Thu 2 Feb 2017
SRR4063896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3656062
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT44600.12198917852049557No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA44120.12067629050054403No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA43910.12010190199181524No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA42890.11731201494941826No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC39810.10888765015472932No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG38030.10401902374740911No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC37730.10319846873493939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49800.024.87091
TAACGGC4150.019.08571436
TTAACGG4650.017.97979235
AACGCAG72200.017.0623746
ATACGAA13300.017.03742440
CGCAATA13750.016.96122436
TCAACGC73700.016.5658664
ACCGTCG11450.016.522958
TACCGTC11900.016.4527197
GGTATCA23850.016.41891
CAACGCA75750.016.2046725
CGTCGTA11500.016.06896410
ATCAACG77300.015.9364393
CAATACG14500.015.93218438
TAGCGGC14650.015.91967230
TCTAGCG14650.015.91945528
TATCAAC78200.015.8653322
ATACCGT12350.015.8530096
TACGAAT14200.015.802875541
GTATTAG10750.015.7577681
Produced by FastQC (version 0.11.5)