##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063896_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3656062
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238002801921848	32.0	32.0	32.0	32.0	32.0
2	31.2366264576476	32.0	32.0	32.0	32.0	32.0
3	31.372641656514578	32.0	32.0	32.0	32.0	32.0
4	31.499345744136726	32.0	32.0	32.0	32.0	32.0
5	31.391543141226816	32.0	32.0	32.0	32.0	32.0
6	35.007072363652476	36.0	36.0	36.0	36.0	36.0
7	35.02990731557616	36.0	36.0	36.0	36.0	36.0
8	34.95700510549329	36.0	36.0	36.0	36.0	36.0
9	35.11258698566928	36.0	36.0	36.0	36.0	36.0
10	34.910897025269264	36.0	36.0	36.0	32.0	36.0
11	35.12118120535155	36.0	36.0	36.0	36.0	36.0
12	34.99609224351228	36.0	36.0	36.0	36.0	36.0
13	35.05754689061619	36.0	36.0	36.0	36.0	36.0
14	34.992729062034506	36.0	36.0	36.0	36.0	36.0
15	34.952511746245	36.0	36.0	36.0	36.0	36.0
16	34.969568076252536	36.0	36.0	36.0	36.0	36.0
17	34.92562407311473	36.0	36.0	36.0	32.0	36.0
18	34.93059335427025	36.0	36.0	36.0	36.0	36.0
19	34.930202496565975	36.0	36.0	36.0	32.0	36.0
20	34.92675534495859	36.0	36.0	36.0	32.0	36.0
21	34.92283500662735	36.0	36.0	36.0	32.0	36.0
22	34.90463536996911	36.0	36.0	36.0	32.0	36.0
23	34.84839835867116	36.0	36.0	36.0	32.0	36.0
24	34.810351684407976	36.0	36.0	36.0	32.0	36.0
25	34.78070886106417	36.0	36.0	36.0	32.0	36.0
26	34.71310770988019	36.0	36.0	36.0	32.0	36.0
27	34.711894109016754	36.0	36.0	36.0	32.0	36.0
28	34.68895111734976	36.0	36.0	36.0	32.0	36.0
29	34.65577252245723	36.0	36.0	36.0	32.0	36.0
30	34.64433425910173	36.0	36.0	36.0	32.0	36.0
31	34.6439064764219	36.0	36.0	36.0	32.0	36.0
32	34.60291920651236	36.0	36.0	36.0	32.0	36.0
33	34.574006403611314	36.0	36.0	36.0	32.0	36.0
34	34.56631479444276	36.0	36.0	36.0	32.0	36.0
35	34.53344445471658	36.0	36.0	36.0	32.0	36.0
36	34.50492907395991	36.0	36.0	36.0	32.0	36.0
37	34.500638391799704	36.0	36.0	36.0	32.0	36.0
38	34.45764486488468	36.0	36.0	36.0	32.0	36.0
39	34.46552219300438	36.0	36.0	36.0	32.0	36.0
40	34.43972011415561	36.0	36.0	36.0	32.0	36.0
41	34.42351305858599	36.0	36.0	36.0	32.0	36.0
42	34.34896235348307	36.0	36.0	36.0	32.0	36.0
43	34.353483611601774	36.0	36.0	36.0	32.0	36.0
44	34.291012570355754	36.0	36.0	36.0	32.0	36.0
45	34.26220343090462	36.0	36.0	36.0	32.0	36.0
46	34.2538737034547	36.0	36.0	36.0	32.0	36.0
47	34.22608095814568	36.0	36.0	36.0	32.0	36.0
48	34.186599133165686	36.0	36.0	36.0	32.0	36.0
49	34.1798024759974	36.0	36.0	36.0	32.0	36.0
50	33.52081994233139	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	4.0
19	16.0
20	34.0
21	116.0
22	403.0
23	1113.0
24	2975.0
25	6860.0
26	13597.0
27	25329.0
28	41943.0
29	64975.0
30	95666.0
31	139988.0
32	208252.0
33	342916.0
34	711723.0
35	2000149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.0862896007808	18.194311624252954	12.186328553265334	26.53307022170091
2	16.348316758022342	20.171114101596263	36.147443682235426	27.333125458145975
3	17.910317941581198	24.221037518917278	28.366222662770657	29.502421876730867
4	12.461659567042354	16.026369355880725	35.4935993973844	36.01837167969252
5	15.010576954110736	36.452992317963975	32.5631786331851	15.973252094740186
6	34.990920546370035	35.208723496378745	16.37630376112845	13.424052196122762
7	31.021833922584296	30.0358528621295	20.47428730538444	18.46802590990177
8	28.893766024755596	32.06201098340236	18.834281256718295	20.20994173512375
9	27.63771016068875	14.06770546704989	18.037659613808707	40.25692475845265
10	16.29695294919868	26.157821044538238	30.555244805358384	26.9899812009047
11	37.89313517455139	20.70057422502597	22.038463284772835	19.36782731564981
12	25.465712962666654	23.110473266173624	27.814005291487202	23.609808479672523
13	29.484199173806214	18.971795463018353	25.074650380206144	26.46935498296929
14	24.278231985989276	19.214525375288186	24.289391549420593	32.217851089301945
15	25.753814272592546	26.665415711810635	21.551508613261834	26.02926140233498
16	26.688380547987396	25.250237073899495	22.73275432688928	25.328628051223827
17	24.82952969615942	25.444207456000473	24.286650499909467	25.43961234793064
18	25.78773410472925	24.326569396092403	25.132601907629425	24.753094591548926
19	26.20967587529971	24.442692711447453	24.57116427456646	24.776467138686378
20	26.50374135230254	23.696790317149937	23.9385278735328	25.860940457014724
21	27.705410903863225	23.58589651926034	23.596098753248715	25.112593823627716
22	26.611284613099684	23.537728340195915	24.175026763798606	25.6759602829058
23	24.980880911326818	23.29123486884141	24.972237660939513	26.755646558892266
24	25.495747453874333	24.350712313442532	24.29409375431648	25.85944647836665
25	25.600520350678718	23.80125689415415	24.522510418501554	26.075712336665575
26	25.50141307071262	24.536115636560183	24.7704785840189	25.191992708708298
27	26.11268805345137	24.15681453817746	23.86505968993457	25.865437718436603
28	25.3019235797994	23.960910223824992	24.924396016074873	25.81277018030073
29	25.363316811493753	24.097551155384522	24.850265415270208	25.68886661785152
30	25.233989709868627	24.303419376191556	25.071185717450827	25.391405196488993
31	26.025183093321107	24.055292076724403	23.897952451264924	26.021572378689562
32	25.67032820349826	24.114555709855733	23.744718516449836	26.47039757019617
33	25.091632603044285	23.872809570492002	24.506879830542598	26.528677995921118
34	25.947509186384732	23.827351219408122	24.715053854616333	25.510085739590814
35	26.640287706813893	23.699104800292346	24.534812349423298	25.12579514347047
36	25.403170657251056	24.38286632754997	24.192263631320554	26.021699383878428
37	26.541154858529104	24.086253740859664	24.077446086625194	25.295145313986033
38	25.455463552111024	23.82437569427709	24.683726951915336	26.036433801696546
39	26.14707983715055	23.88429417499204	23.908337554829302	26.060288433028113
40	26.299976832407495	24.146402773108854	24.405595342848912	25.148025051634736
41	25.293310098106016	24.58982538841552	24.707934450837993	25.408930062640472
42	26.7035814385656	24.499909600384466	24.087599825929455	24.708909135120482
43	25.806329851395372	23.587353426276188	24.48067702221821	26.12563970011023
44	25.545354709977943	24.194402922307486	24.079605800304428	26.180636567410144
45	25.622372136634127	24.394148596886446	24.22207585751907	25.761403408960366
46	25.50906422335533	24.18032495428091	24.40458509275929	25.90602572960447
47	25.721174623493564	23.997724276391008	24.720977685873553	25.56012341424187
48	26.56696699653718	24.641464121794975	23.65324755605884	25.138321325609002
49	25.45660679778285	24.857954428897894	23.901593114964395	25.78384565835486
50	25.684538177600963	24.740037281130633	23.923517610068913	25.65190693119949
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	24.5
2	34.0
3	118.5
4	203.0
5	244.0
6	285.0
7	287.5
8	290.0
9	375.0
10	460.0
11	703.0
12	946.0
13	1891.0
14	2836.0
15	3896.5
16	4957.0
17	5231.0
18	5505.0
19	5652.0
20	5799.0
21	6659.5
22	7520.0
23	8546.0
24	9572.0
25	11854.5
26	14137.0
27	18011.0
28	21885.0
29	26457.0
30	31029.0
31	35994.5
32	40960.0
33	47929.0
34	54898.0
35	66268.0
36	77638.0
37	97383.0
38	117128.0
39	132193.0
40	147258.0
41	164639.5
42	182021.0
43	187493.0
44	192965.0
45	214851.0
46	236737.0
47	255018.0
48	273299.0
49	285050.5
50	296802.0
51	287291.5
52	277781.0
53	276417.5
54	275054.0
55	278861.5
56	282669.0
57	276722.5
58	270776.0
59	248293.0
60	225810.0
61	199449.5
62	173089.0
63	150607.5
64	128126.0
65	109395.0
66	90664.0
67	78759.0
68	66854.0
69	60681.0
70	54508.0
71	42228.5
72	29949.0
73	25431.5
74	20914.0
75	16427.0
76	11940.0
77	10448.0
78	8956.0
79	7335.0
80	5714.0
81	4605.5
82	3497.0
83	2934.0
84	2371.0
85	1707.0
86	1043.0
87	738.0
88	433.0
89	346.0
90	259.0
91	211.0
92	163.0
93	125.5
94	88.0
95	97.5
96	107.0
97	81.0
98	55.0
99	59.0
100	63.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008916697802170751
2	6.017403424777807E-4
3	3.555738387368704E-4
4	0.0
5	0.0
6	1.3675916874495017E-4
7	2.1881466999192027E-4
8	0.0
9	0.0013675916874495017
10	9.573141812146512E-4
11	1.0940733499596013E-4
12	2.7351833748990034E-5
13	5.196848412308107E-4
14	1.3675916874495017E-4
15	0.005142144744810126
16	7.93203178720711E-4
17	0.0
18	1.641110024939402E-4
19	0.0
20	8.47906846218691E-4
21	0.0
22	1.0940733499596013E-4
23	8.20555012469701E-4
24	4.6498117373283053E-4
25	0.0018052210274333424
26	0.005306255747304067
27	0.006920013938494479
28	0.004649811737328305
29	0.002543720538656073
30	0.0036104420548666847
31	0.00754910611472125
32	0.0035283865536197144
33	0.004321589732340425
34	0.0029539980448909237
35	0.0032275163823808238
36	0.00793203178720711
37	0.004130126896097494
38	0.007685865283466199
39	0.004649811737328305
40	0.002543720538656073
41	0.003254868216129814
42	0.0019966838636762724
43	0.0014222953549474816
44	0.0012855361862025316
45	0.0013675916874495017
46	0.0010940733499596013
47	0.002242850367417183
48	0.002242850367417183
49	4.6498117373283053E-4
50	0.001559054523692432
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3656062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.5596193866511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.51385765371708	33.948229937344486
2	13.798332174880393	12.572935241162646
3	4.740329370237986	6.479028056262185
4	2.1636116428082586	3.9429329178748467
5	1.1972548985288372	2.72732387428887
6	0.7208191376830654	1.9704147335672706
7	0.49591838914466435	1.5815697139390426
8	0.3544632390445239	1.2919368205942428
9	0.27260524471233044	1.1177812072708793
>10	1.5438251221854853	12.832457216274996
>50	0.10318727674064074	3.256269585966129
>100	0.07323198799177337	6.768643207909163
>500	0.014057165570418001	4.425076943727739
>1k	0.008506696754648518	7.085400543817605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	4460	0.12198917852049557	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	4412	0.12067629050054403	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	4391	0.12010190199181524	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	4289	0.11731201494941826	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3981	0.10888765015472932	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	3803	0.10401902374740911	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3773	0.10319846873493939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1881466999192027E-4
2	0.0	0.0	0.0	0.0	2.1881466999192027E-4
3	0.0	0.0	0.0	0.0	2.1881466999192027E-4
4	0.0	0.0	0.0	0.0	2.1881466999192027E-4
5	0.0	0.0	0.0	0.0	2.461665037409103E-4
6	0.0	0.0	0.0	0.0	3.008701712388904E-4
7	0.0	0.0	0.0	0.0	3.282220049878804E-4
8	0.0	0.0	0.0	0.0	3.5557383873687046E-4
9	0.0	0.0	0.0	0.0	4.102775062348505E-4
10	0.0	0.0	0.0	0.0	4.102775062348505E-4
11	0.0	0.0	0.0	0.0	4.102775062348505E-4
12	0.0	0.0	0.0	0.0	7.658513449717209E-4
13	0.0	0.0	0.0	5.470366749798007E-5	7.93203178720711E-4
14	0.0	0.0	0.0	5.470366749798007E-5	7.93203178720711E-4
15	0.0	0.0	0.0	1.0940733499596013E-4	0.0010393696824616213
16	0.0	0.0	0.0	1.0940733499596013E-4	0.0011761288512065714
17	0.0	0.0	0.0	1.0940733499596013E-4	0.0012308325187045516
18	0.0	0.0	0.0	1.641110024939402E-4	0.0012581843524535415
19	0.0	0.0	0.0	1.641110024939402E-4	0.0012581843524535415
20	0.0	0.0	0.0	2.1881466999192027E-4	0.0013675916874495017
21	0.0	0.0	0.0	3.008701712388904E-4	0.0014496471886964717
22	5.470366749798007E-5	0.0	0.0	4.3762933998384054E-4	0.0014769990224454618
23	5.470366749798007E-5	0.0	0.0	7.658513449717209E-4	0.001559054523692432
24	5.470366749798007E-5	0.0	0.0	0.0012855361862025316	0.001586406357441422
25	5.470366749798007E-5	0.0	0.0	0.0015317026899434418	0.001586406357441422
26	5.470366749798007E-5	0.0	0.0	0.0019146283624293023	0.001668461858688392
27	5.470366749798007E-5	0.0	0.0	0.0022154985336681927	0.001668461858688392
28	5.470366749798007E-5	0.0	0.0	0.0035283865536197144	0.001668461858688392
29	5.470366749798007E-5	0.0	0.0	0.005661829586040937	0.001668461858688392
30	5.470366749798007E-5	0.0	0.0	0.009627845479644492	0.001668461858688392
31	5.470366749798007E-5	0.0	0.0	0.019802727634268786	0.0017505173599353622
32	5.470366749798007E-5	0.0	0.0	0.03241192299255319	0.0017505173599353622
33	5.470366749798007E-5	0.0	0.0	0.04447408167585779	0.0017505173599353622
34	5.470366749798007E-5	0.0	0.0	0.05910731273156746	0.0018052210274333421
35	5.470366749798007E-5	0.0	0.0	0.0751901909759736	0.0018052210274333421
36	5.470366749798007E-5	0.0	0.0	0.09876747166760301	0.0018052210274333421
37	5.470366749798007E-5	0.0	0.0	0.13385987436755722	0.0018052210274333421
38	5.470366749798007E-5	0.0	0.0	0.18539072915065444	0.0019146283624293023
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4980	0.0	24.8709	1
TAACGGC	415	0.0	19.085714	36
TTAACGG	465	0.0	17.979792	35
AACGCAG	7220	0.0	17.062374	6
ATACGAA	1330	0.0	17.037424	40
CGCAATA	1375	0.0	16.961224	36
TCAACGC	7370	0.0	16.565866	4
ACCGTCG	1145	0.0	16.52295	8
TACCGTC	1190	0.0	16.452719	7
GGTATCA	2385	0.0	16.4189	1
CAACGCA	7575	0.0	16.204672	5
CGTCGTA	1150	0.0	16.068964	10
ATCAACG	7730	0.0	15.936439	3
CAATACG	1450	0.0	15.932184	38
TAGCGGC	1465	0.0	15.919672	30
TCTAGCG	1465	0.0	15.919455	28
TATCAAC	7820	0.0	15.865332	2
ATACCGT	1235	0.0	15.853009	6
TACGAAT	1420	0.0	15.8028755	41
GTATTAG	1075	0.0	15.757768	1
>>END_MODULE