FastQCFastQC Report
Thu 2 Feb 2017
SRR4063895_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063895_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830247
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT237901.2998245591988402No Hit
GTCCTACAGTGGACATTTCTAAATT209011.141977011845942No Hit
CTGTAGGACGTGGAATATGGCAAGA204091.1150953942282107No Hit
CTTTAGGACGTGAAATATGGCGAGG199561.0903446365435923No Hit
GTCCTACAGTGTGCATTTCTCATTT142860.7805503847294928No Hit
CTGTAGGACCTGGAATATGGCGAGA107910.5895925522620717No Hit
CTGAAGGACCTGGAATATGGCGAGA103480.565388168919277No Hit
ATTTAGAAATGTCCACTGTAGGACG100730.5503628745191222No Hit
GTCCTTCAGTGTGCATTTCTCATTT86320.47163033186231146No Hit
TTTCTAAATTTTCCACCTTTTTCAG81890.4474259485195167No Hit
GAATATGGCAAGAAAACTGAAAATC68010.37158918987437217No Hit
GGAATATGGCGAGAAAACTGAAAAT56940.31110555023447656No Hit
GCCATATTCCACGTCCTACAGTGGA49890.27258615913589807No Hit
CTGTAGGACATGGAATATGGCAAGA49160.2685976264405842No Hit
GAAATATGGCGAGGAAAACTGAAAA48680.26597502959983No Hit
ATTCCAGGTCCTTCAGTGTGCATTT46900.2562495663153662No Hit
CCATATTCCAGGTCCTTCAGTGTGC43900.2398583360606519No Hit
ACAGTGGACATTTCTAAATTTTCCA43380.23701718948316813No Hit
GTCCACTGTAGGACGTGGAATATGG42070.2298596856052762No Hit
CCATATTTCACGTCCTAAAGTGTGT40790.2228660940299315No Hit
TTCCAGGTCCTTCAGTGTGCATTTC40680.2222650822539253No Hit
GATATACACTGTTCTACAAATCCCG40150.2193692982422591No Hit
ACCTGGAATATGGCGAGAAAACTGA38670.2112829579832667No Hit
ATACACACTTTAGGACGTGAAATAT38610.21095513337817243No Hit
CCTAAAGTGTGTATTTCTCATTTTC37380.20423472897373962No Hit
GACCTGGAATATGGCGAGAAAACTG36940.20183068186971484No Hit
CACTTTAGGACGTGAAATATGGCGA36450.19915344759477818No Hit
TTGTAGAACAGTGTATATCAATGAG35220.19243304319034535No Hit
CCACTGTAGGACGTGGAATATGGCA35010.19128565707251535No Hit
GTGTATTTCTCATTTTCCGTGATTT34990.1911763822041506No Hit
GAAATACACACTTTAGGACGTGAAA34310.1874610366797487No Hit
TCCTAAAGTGTGTATTTCTCATTTT34140.18653220029864823No Hit
ATTCCACGTCCTACAGTGGACATTT30320.16566070044097872No Hit
CTACAGTGGACATTTCTAAATTTTC30320.16566070044097872No Hit
GTATCAACGCAGAGTACTTTTTTTT29820.16292882873185968No Hit
GTAGGACGTGGAATATGGCAAGAAA29660.1620546297849416No Hit
GTGTATATCAATGAGTTACAATGAA28190.15402292696013162No Hit
AGTGTGTATTTCTCATTTTCCGTGA28000.15298481571066638No Hit
TTTCTAAATATTCCACCTTTTTCAG27120.14817672150261688No Hit
GGTCCTTCAGTGTGCATTTCTCATT26700.14588194926695686No Hit
ATATTTCACGTCCTAAAGTGTGTAT26380.14413355137312067No Hit
GATATACACTGTTCTACAATGCCGG25870.14134704222981925No Hit
GTGTATATCAATGAGTTACAATGAG25400.13877908282324736No Hit
GTCCTACAGTGGACATTTCTAAATA25390.13872444538906498No Hit
GTTCTACAGTGTGGTTTTTATCATT23710.12954535644642498No Hit
GTATTTCTCATTTTCCGTGATTTTC23210.12681348473730594No Hit
GAGAAATACACACTTTAGGACGTGA21560.1177983080972131No Hit
TTTTTCAAGTCGTCAAGTGGATGTT21390.11686947171611263No Hit
GTATCAACGCAGAGTACATGGGAAA21120.11539426099318835No Hit
CACTGAAGGACCTGGAATATGGCGA20880.1140829625728112No Hit
ACGTGGAATATGGCAAGAAAACTGA20730.11326340106007551No Hit
GTGAAATATGGCGAGGAAAACTGAA20710.11315412619171074No Hit
GTGCATTTCTCATTTTTCACGTTTT20610.11260775184988693No Hit
CTGTAGAACATATTAGATGAGTGAG20560.11233456467897503No Hit
CTTTAGGACATGAAATATGGCGAGG20200.1103676170484093No Hit
TATCAACGCAGAGTACTTTTTTTTT20090.10976660527240313No Hit
GTGTGTATTTCTCATTTTCCGTGAT19610.10714400843164884No Hit
ACGTCCTACAGTGGACATTTCTAAA19160.1046853238934417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTT402.761239E-416.62516812
AAGCCGG350.002167477516.28899818
ATGTCCG508.710471E-515.2009849
ACGTAGA456.761221E-414.7783318
TGTCCGC456.761221E-414.77833110
ACGGCGA653.365869E-614.61832917
TACGGCG653.3696906E-614.61673116
TTTGGCG653.3696906E-614.61673116
ATTGGCG400.00527634914.25131316
CCACCTT22850.013.84439913
GCGTTTC551.9607383E-413.81794313
TAGAAAT26150.013.7665234
CACCTTT23100.013.69569214
GGCGAGC707.247072E-613.57453519
TAGGACC40100.013.5490364
ACCTTTT24000.013.4991615
ATAACGA500.00149744913.3026817
CAACTTT3100.013.17827214
TTCCACC25450.013.1769511
CGGCGAG655.443218E-513.15649618
Produced by FastQC (version 0.11.5)