##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063895_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1830247
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.386110180757022	32.0	32.0	32.0	32.0	32.0
2	31.091187145778683	32.0	32.0	32.0	32.0	32.0
3	31.10507106417877	32.0	32.0	32.0	32.0	32.0
4	31.114302058683883	32.0	32.0	32.0	32.0	32.0
5	31.132058678418815	32.0	32.0	32.0	32.0	32.0
6	34.8052486904773	36.0	36.0	36.0	36.0	36.0
7	34.737232187786674	36.0	36.0	36.0	36.0	36.0
8	34.714349483976754	36.0	36.0	36.0	32.0	36.0
9	34.761286864559814	36.0	36.0	36.0	32.0	36.0
10	34.5451056606021	36.0	36.0	36.0	32.0	36.0
11	34.70529879300444	36.0	36.0	36.0	32.0	36.0
12	34.586234535557224	36.0	36.0	36.0	32.0	36.0
13	34.62605648308671	36.0	36.0	36.0	32.0	36.0
14	34.60072219760502	36.0	36.0	36.0	32.0	36.0
15	34.57712592890468	36.0	36.0	36.0	32.0	36.0
16	34.55790338681063	36.0	36.0	36.0	32.0	36.0
17	34.543708035035706	36.0	36.0	36.0	32.0	36.0
18	34.5409018564161	36.0	36.0	36.0	32.0	36.0
19	34.41351959598896	36.0	36.0	36.0	32.0	36.0
20	34.361841871616235	36.0	36.0	36.0	32.0	36.0
21	34.330299134488406	36.0	36.0	36.0	32.0	36.0
22	34.2557862408735	36.0	36.0	36.0	32.0	36.0
23	34.30414965848872	36.0	36.0	36.0	32.0	36.0
24	34.2110469242676	36.0	36.0	36.0	32.0	36.0
25	33.894642635666116	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	13.0
5	23.0
6	67.0
7	28.0
8	125.0
9	130.0
10	163.0
11	49.0
12	112.0
13	117.0
14	172.0
15	319.0
16	474.0
17	586.0
18	816.0
19	1093.0
20	1431.0
21	2029.0
22	3186.0
23	4890.0
24	7344.0
25	10608.0
26	15051.0
27	20409.0
28	28820.0
29	39060.0
30	52536.0
31	73639.0
32	106836.0
33	156908.0
34	372614.0
35	930595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.01477984104019	20.636353052359663	12.90382882058071	26.44503828601944
2	10.078021310926315	19.969499617789012	52.15816704833125	17.794312022953424
3	18.89732932432381	26.817672934978535	30.412941458662416	23.87205628203524
4	9.881956578991943	18.099590530736425	41.415570322821765	30.602882567449868
5	8.97694822120003	42.12334717991928	38.26045570240004	10.63924889648065
6	28.26514971434192	40.74459626718252	19.79967048910237	11.19058352937319
7	25.99998797680836	31.82068830586125	23.598246144244417	18.581077573085977
8	20.262797580819274	48.05779579075672	19.4438507935033	12.235555834920705
9	26.70419634091143	14.707146090215534	21.655675260809005	36.932982308064034
10	15.714158411024117	29.246377968382088	36.8130495371355	18.2264140834583
11	32.38506245117372	18.69052350522554	32.96318751096797	15.96122653263277
12	28.313439429294995	22.31601783671836	35.77928482544904	13.591257908537614
13	31.765297642479112	24.438831299479506	26.102709618160347	17.69316143988103
14	16.60799139198455	28.510989380495168	27.382499550298988	27.49851967722129
15	17.222979718182348	45.788371058090036	21.12664737147687	15.862001852250748
16	15.243712742150391	27.54228183295908	39.43242588616478	17.781579538725747
17	16.312268836810066	34.35219634075334	32.84913330034745	16.486401522089157
18	16.471246976841087	26.300867399861875	42.933149022938004	14.294736600359034
19	25.121429098693437	24.17208675420826	27.74609111215002	22.960393034948286
20	24.51210162940831	29.915757217177525	31.08195598989918	14.49018516351499
21	19.23919634864991	26.661373586985587	26.910771690553876	27.188658373810632
22	23.133467739951083	36.03797943874027	26.678510634691232	14.150042186617409
23	15.955184964826936	35.74090924999125	32.097937738424385	16.20596804675743
24	24.611649423653187	27.438045451562807	33.28599706905225	14.664308055731754
25	19.171709195976767	32.19116809147967	33.36703525523601	15.270087457307557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	146.0
1	146.0
2	366.5
3	587.0
4	587.0
5	587.0
6	1517.5
7	2448.0
8	2448.0
9	2448.0
10	2703.5
11	2959.0
12	2959.0
13	2959.0
14	4181.0
15	5403.0
16	5403.0
17	5403.0
18	11370.5
19	17338.0
20	17338.0
21	17338.0
22	38218.0
23	59098.0
24	59098.0
25	59098.0
26	105840.0
27	152582.0
28	152582.0
29	152582.0
30	215031.5
31	277481.0
32	277481.0
33	277481.0
34	284533.5
35	291586.0
36	291586.0
37	291586.0
38	283220.0
39	274854.0
40	274854.0
41	274854.0
42	262521.5
43	250189.0
44	250189.0
45	250189.0
46	241201.0
47	232213.0
48	232213.0
49	232213.0
50	191859.0
51	151505.0
52	151505.0
53	151505.0
54	104609.0
55	57713.0
56	57713.0
57	57713.0
58	42672.0
59	27631.0
60	27631.0
61	27631.0
62	20856.5
63	14082.0
64	14082.0
65	14082.0
66	10441.5
67	6801.0
68	6801.0
69	6801.0
70	4842.0
71	2883.0
72	2883.0
73	2883.0
74	2050.5
75	1218.0
76	1218.0
77	1218.0
78	826.5
79	435.0
80	435.0
81	435.0
82	293.5
83	152.0
84	152.0
85	152.0
86	116.0
87	80.0
88	80.0
89	80.0
90	75.0
91	70.0
92	70.0
93	70.0
94	70.5
95	71.0
96	71.0
97	71.0
98	396.5
99	722.0
100	722.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03977605208477326
2	0.00579156802333237
3	0.0019669476305657105
4	0.003988532695313802
5	0.010545024797199503
6	0.014315007755783782
7	0.02458684538207138
8	0.03835547879603136
9	0.05130455069725561
10	0.05977335299552465
11	0.05780640536495894
12	0.06616593279486321
13	0.06594738305813368
14	0.06851534246470559
15	0.061248563718448924
16	0.06938954141162368
17	0.06458144720357416
18	0.07911500469608747
19	0.07660168272369794
20	0.08113658976083556
21	0.07889645495935795
22	0.08020775337973508
23	0.08605395883724984
24	0.08124586462920032
25	0.08026239081391746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1830247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.4978106283728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02151862792631	25.13056624051218
2	13.759238313992247	9.218087188655275
3	4.402470304170222	4.424193496383866
4	1.897222390806829	2.542111854686234
5	1.0648796378680219	1.7835568225656597
6	0.6469901106147335	1.3003651322281389
7	0.4681559120107561	1.0977538659562627
8	0.35153336705953264	0.9420478527451592
9	0.26671498390090187	0.804093122021572
>10	1.723034961112372	11.286957969219557
>50	0.1924872199299811	4.566568144330039
>100	0.1647341036639873	11.083594480870673
>500	0.02173573268351303	4.89534659178892
>1k	0.017323215522198353	12.172782783995089
>5k	6.537062461206926E-4	1.6048844879882351
>10k+	0.0013074124922413852	7.147089966053215
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	23790	1.2998245591988402	No Hit
GTCCTACAGTGGACATTTCTAAATT	20901	1.141977011845942	No Hit
CTGTAGGACGTGGAATATGGCAAGA	20409	1.1150953942282107	No Hit
CTTTAGGACGTGAAATATGGCGAGG	19956	1.0903446365435923	No Hit
GTCCTACAGTGTGCATTTCTCATTT	14286	0.7805503847294928	No Hit
CTGTAGGACCTGGAATATGGCGAGA	10791	0.5895925522620717	No Hit
CTGAAGGACCTGGAATATGGCGAGA	10348	0.565388168919277	No Hit
ATTTAGAAATGTCCACTGTAGGACG	10073	0.5503628745191222	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	8632	0.47163033186231146	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	8189	0.4474259485195167	No Hit
GAATATGGCAAGAAAACTGAAAATC	6801	0.37158918987437217	No Hit
GGAATATGGCGAGAAAACTGAAAAT	5694	0.31110555023447656	No Hit
GCCATATTCCACGTCCTACAGTGGA	4989	0.27258615913589807	No Hit
CTGTAGGACATGGAATATGGCAAGA	4916	0.2685976264405842	No Hit
GAAATATGGCGAGGAAAACTGAAAA	4868	0.26597502959983	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	4690	0.2562495663153662	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	4390	0.2398583360606519	No Hit
ACAGTGGACATTTCTAAATTTTCCA	4338	0.23701718948316813	No Hit
GTCCACTGTAGGACGTGGAATATGG	4207	0.2298596856052762	No Hit
CCATATTTCACGTCCTAAAGTGTGT	4079	0.2228660940299315	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	4068	0.2222650822539253	No Hit
GATATACACTGTTCTACAAATCCCG	4015	0.2193692982422591	No Hit
ACCTGGAATATGGCGAGAAAACTGA	3867	0.2112829579832667	No Hit
ATACACACTTTAGGACGTGAAATAT	3861	0.21095513337817243	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	3738	0.20423472897373962	No Hit
GACCTGGAATATGGCGAGAAAACTG	3694	0.20183068186971484	No Hit
CACTTTAGGACGTGAAATATGGCGA	3645	0.19915344759477818	No Hit
TTGTAGAACAGTGTATATCAATGAG	3522	0.19243304319034535	No Hit
CCACTGTAGGACGTGGAATATGGCA	3501	0.19128565707251535	No Hit
GTGTATTTCTCATTTTCCGTGATTT	3499	0.1911763822041506	No Hit
GAAATACACACTTTAGGACGTGAAA	3431	0.1874610366797487	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	3414	0.18653220029864823	No Hit
ATTCCACGTCCTACAGTGGACATTT	3032	0.16566070044097872	No Hit
CTACAGTGGACATTTCTAAATTTTC	3032	0.16566070044097872	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2982	0.16292882873185968	No Hit
GTAGGACGTGGAATATGGCAAGAAA	2966	0.1620546297849416	No Hit
GTGTATATCAATGAGTTACAATGAA	2819	0.15402292696013162	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	2800	0.15298481571066638	No Hit
TTTCTAAATATTCCACCTTTTTCAG	2712	0.14817672150261688	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	2670	0.14588194926695686	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	2638	0.14413355137312067	No Hit
GATATACACTGTTCTACAATGCCGG	2587	0.14134704222981925	No Hit
GTGTATATCAATGAGTTACAATGAG	2540	0.13877908282324736	No Hit
GTCCTACAGTGGACATTTCTAAATA	2539	0.13872444538906498	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2371	0.12954535644642498	No Hit
GTATTTCTCATTTTCCGTGATTTTC	2321	0.12681348473730594	No Hit
GAGAAATACACACTTTAGGACGTGA	2156	0.1177983080972131	No Hit
TTTTTCAAGTCGTCAAGTGGATGTT	2139	0.11686947171611263	No Hit
GTATCAACGCAGAGTACATGGGAAA	2112	0.11539426099318835	No Hit
CACTGAAGGACCTGGAATATGGCGA	2088	0.1140829625728112	No Hit
ACGTGGAATATGGCAAGAAAACTGA	2073	0.11326340106007551	No Hit
GTGAAATATGGCGAGGAAAACTGAA	2071	0.11315412619171074	No Hit
GTGCATTTCTCATTTTTCACGTTTT	2061	0.11260775184988693	No Hit
CTGTAGAACATATTAGATGAGTGAG	2056	0.11233456467897503	No Hit
CTTTAGGACATGAAATATGGCGAGG	2020	0.1103676170484093	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2009	0.10976660527240313	No Hit
GTGTGTATTTCTCATTTTCCGTGAT	1961	0.10714400843164884	No Hit
ACGTCCTACAGTGGACATTTCTAAA	1916	0.1046853238934417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.463743418238085E-5	0.0
11	0.0	0.0	0.0	5.463743418238085E-5	0.0
12	0.0	0.0	0.0	5.463743418238085E-5	0.0
13	0.0	0.0	0.0	5.463743418238085E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTTT	40	2.761239E-4	16.625168	12
AAGCCGG	35	0.0021674775	16.288998	18
ATGTCCG	50	8.710471E-5	15.200984	9
ACGTAGA	45	6.761221E-4	14.778331	8
TGTCCGC	45	6.761221E-4	14.778331	10
ACGGCGA	65	3.365869E-6	14.618329	17
TACGGCG	65	3.3696906E-6	14.616731	16
TTTGGCG	65	3.3696906E-6	14.616731	16
ATTGGCG	40	0.005276349	14.251313	16
CCACCTT	2285	0.0	13.844399	13
GCGTTTC	55	1.9607383E-4	13.817943	13
TAGAAAT	2615	0.0	13.766523	4
CACCTTT	2310	0.0	13.695692	14
GGCGAGC	70	7.247072E-6	13.574535	19
TAGGACC	4010	0.0	13.549036	4
ACCTTTT	2400	0.0	13.49916	15
ATAACGA	50	0.001497449	13.30268	17
CAACTTT	310	0.0	13.178272	14
TTCCACC	2545	0.0	13.17695	11
CGGCGAG	65	5.443218E-5	13.156496	18
>>END_MODULE