FastQCFastQC Report
Thu 2 Feb 2017
SRR4063895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830247
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA107190.5856586570009403No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA88570.48392375455334713No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA87300.4769848004121848No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC76270.41671971050901874No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA57620.31482089575887845No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA51340.2805085870923433No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT36510.19948127219987247No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA34980.1911217447699682No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA32060.175167613988713No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA29020.15855783399726922No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG25620.13998110637525973No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT25550.13959864433598307No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG23750.1297639061831545No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA23390.1277969585525888No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC23340.1275237713816769No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG22140.12096727927979119No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA21630.11818077013648978No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT20020.10938414323312647No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATAT19760.10796356994438455No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCAC19320.1055595228403598No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATG18760.10249982652614648No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATT18730.10233591422359933No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG18610.10168026501341076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC44900.027.6325824
TATAGGA7700.025.1408922
GACCGGG801.3115823E-724.7480647
GTATCAA33700.024.6113681
GCGAGGG450.001398457824.44453620
ATAGGAC7800.024.2545133
TACGACT651.8151499E-523.6904544
GTCCTAT6650.023.488811
AGGACCG852.3558096E-723.2922955
ACTGTTC18900.022.3474588
AGGACCT88150.022.3102235
GGACCTG85900.022.228766
TCCTATA7200.021.6927472
CGGTAGG1551.8189894E-1221.2904011
CTAGGAC8200.021.1934643
GACCTAG3150.020.9507437
TGTAGGA144250.020.8621462
TTAGGAC86650.020.8177593
GATATAC22500.020.7289661
CGAGGAG856.1146166E-620.70652621
Produced by FastQC (version 0.11.5)