##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063895_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1830247
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39391568460432	32.0	32.0	32.0	32.0	32.0
2	31.523881202919608	32.0	32.0	32.0	32.0	32.0
3	31.58383103482754	32.0	32.0	32.0	32.0	32.0
4	31.64491841811515	32.0	32.0	32.0	32.0	32.0
5	31.631363963443185	32.0	32.0	32.0	32.0	32.0
6	35.24553899009259	36.0	36.0	36.0	36.0	36.0
7	35.26903855053444	36.0	36.0	36.0	36.0	36.0
8	35.20153290785342	36.0	36.0	36.0	36.0	36.0
9	35.30169698406827	36.0	36.0	36.0	36.0	36.0
10	35.19840983211556	36.0	36.0	36.0	36.0	36.0
11	35.29086128812122	36.0	36.0	36.0	36.0	36.0
12	35.2548404668878	36.0	36.0	36.0	36.0	36.0
13	35.26074185615384	36.0	36.0	36.0	36.0	36.0
14	35.21605280598739	36.0	36.0	36.0	36.0	36.0
15	35.186984051879335	36.0	36.0	36.0	36.0	36.0
16	35.22161489678715	36.0	36.0	36.0	36.0	36.0
17	35.23565398550032	36.0	36.0	36.0	36.0	36.0
18	35.21764767269117	36.0	36.0	36.0	36.0	36.0
19	35.15546043785347	36.0	36.0	36.0	36.0	36.0
20	35.13221767335229	36.0	36.0	36.0	36.0	36.0
21	35.09527457222987	36.0	36.0	36.0	36.0	36.0
22	35.03488873359716	36.0	36.0	36.0	36.0	36.0
23	35.05546355218722	36.0	36.0	36.0	36.0	36.0
24	35.033352875322294	36.0	36.0	36.0	36.0	36.0
25	34.9551836446119	36.0	36.0	36.0	36.0	36.0
26	34.9314076187531	36.0	36.0	36.0	36.0	36.0
27	34.80934540529229	36.0	36.0	36.0	36.0	36.0
28	34.72675873802825	36.0	36.0	36.0	32.0	36.0
29	34.68594730656572	36.0	36.0	36.0	32.0	36.0
30	34.58359718660924	36.0	36.0	36.0	32.0	36.0
31	34.58049432672202	36.0	36.0	36.0	32.0	36.0
32	34.539852271305456	36.0	36.0	36.0	32.0	36.0
33	34.641364253021585	36.0	36.0	36.0	32.0	36.0
34	34.592011351473325	36.0	36.0	36.0	32.0	36.0
35	34.57049075889757	36.0	36.0	36.0	32.0	36.0
36	34.449446987209924	36.0	36.0	36.0	32.0	36.0
37	34.271285788202356	36.0	36.0	36.0	32.0	36.0
38	34.22525757452409	36.0	36.0	36.0	32.0	36.0
39	33.89338105731085	36.0	36.0	36.0	32.0	36.0
40	34.08582502798802	36.0	36.0	36.0	32.0	36.0
41	34.067172900706844	36.0	36.0	36.0	32.0	36.0
42	34.081712468317114	36.0	36.0	36.0	32.0	36.0
43	34.11337977879489	36.0	36.0	36.0	32.0	36.0
44	33.90765945798572	36.0	36.0	36.0	32.0	36.0
45	33.87209540570207	36.0	36.0	36.0	32.0	36.0
46	33.60106313519432	36.0	36.0	36.0	21.0	36.0
47	33.649446222285846	36.0	36.0	36.0	27.0	36.0
48	33.58683117633849	36.0	36.0	36.0	21.0	36.0
49	33.51107528109594	36.0	36.0	36.0	21.0	36.0
50	33.14973771299721	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	10.0
21	30.0
22	114.0
23	409.0
24	1148.0
25	3011.0
26	6608.0
27	13347.0
28	23161.0
29	35559.0
30	50550.0
31	71601.0
32	102957.0
33	166614.0
34	333743.0
35	1021379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.316578383984314	21.55060963681885	13.294366555816985	26.83844542337985
2	9.875338480939071	20.620455504098924	51.28327712928824	18.22092888567376
3	18.557129119678294	27.401652242328872	30.819728560188832	23.221490077804006
4	9.84801504933487	18.80287196209036	41.71874069456199	29.630372294012773
5	8.800232974019353	42.61818213607235	37.793314235728836	10.788270654179463
6	28.692084098260338	40.705863711862925	19.432478800594456	11.169573389282279
7	26.110916237489384	31.94679840851995	23.723696158877004	18.218589195113662
8	20.40096227449082	48.04876063176172	19.30651983038355	12.243757263363907
9	26.278707504588226	14.71451355487598	21.729435747587594	37.277343192948194
10	15.761214319316675	29.632225947928863	36.17392815208168	18.43263158067278
11	32.10852530206322	18.819492024569374	33.152592602305916	15.919390071061484
12	28.26712081401213	22.50280427243814	35.62592508207926	13.604149831470465
13	31.81139679407114	24.291593315390273	26.182881533181003	17.714128357357584
14	16.735728647609058	28.669955852784334	27.2901914503016	27.304124049305013
15	17.372598431392262	45.68408847760685	21.058837542988094	15.884475548012794
16	15.503271987774234	27.653910731584446	38.827351490220096	18.01546579042122
17	16.518917938398477	34.69575417962712	32.22346492030857	16.56186296166583
18	16.738187341550834	26.521439811172304	42.485466386921935	14.254906460354928
19	25.416146244821736	24.199260645836677	28.054152283354263	22.330440825987328
20	24.86664280816398	30.081874731933333	30.729496485424	14.321985974478688
21	19.370390990942752	26.483146810239273	27.254299556289396	26.89216264252858
22	23.51810823141164	35.4780370933946	26.934317536723224	14.069537138470533
23	16.072626699507605	35.73009163845537	31.916703638219335	16.280578023817693
24	24.933492365213105	27.493592373900487	32.740824842192914	14.832090418693495
25	19.464924790051416	32.275859054425446	32.85551931199152	15.403696843531616
26	13.8003405211416	34.79730468118216	34.67031958280696	16.732035214869278
27	16.477663286571527	34.124863533986186	31.48635667708125	17.91111650236104
28	15.25682572830626	34.443776018743606	28.747368415301082	21.55202983764905
29	19.96688877414702	29.321957496332356	27.36812197607359	23.34303175344704
30	15.779005838814623	29.95733720329194	32.00436463140502	22.259292326488417
31	23.28529768061728	26.58899873722678	30.580039659056695	19.545663923099244
32	17.784540907066773	33.587915975707375	30.841035195895493	17.786507921330358
33	14.851512739071019	34.89001216835496	35.176490322961364	15.08198476961266
34	18.11414012432596	33.00353132927801	34.047194032966	14.835134513430035
35	15.971253132202154	34.898605712437345	32.71248433215931	16.41765682320119
36	14.571969999158508	31.816060701105197	35.787358162093284	17.82461113764301
37	16.19399861652801	32.09649553212857	27.947281630529925	23.762224220813494
38	18.469554916118515	36.70950263430819	28.011804935516547	16.809137514056747
39	22.818568619297842	28.780496600576015	29.359133585369175	19.041801194756967
40	24.391852426170193	28.383024922562644	32.31562423334421	14.90949841792296
41	23.735341589725152	25.994192914568963	34.024777917776305	16.24568757792958
42	19.031531900710846	34.642964468558255	32.127460783188816	14.198042847542085
43	14.691919045665077	35.91989365195493	33.32604822269709	16.0621390796829
44	14.458558078136885	34.67335145861927	31.401893975819352	19.46619648742449
45	15.225530017669989	35.177645116275514	30.77675822932956	18.820066636724945
46	18.01642859016164	28.33150477755728	31.946144297091507	21.705922335189573
47	23.79144619944006	28.641069520119068	29.8788550347393	17.688629245701573
48	15.86280978550096	31.691252080353273	29.777163157612918	22.66877497653285
49	21.243334207535625	28.81731357636157	29.856302998513858	20.08304921758895
50	16.273135816634568	36.99976778811349	27.857831687360846	18.869264707891105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24.0
1	18.0
2	12.0
3	50.5
4	89.0
5	98.0
6	107.0
7	134.0
8	161.0
9	206.5
10	252.0
11	405.5
12	559.0
13	1072.0
14	1585.0
15	2323.0
16	3061.0
17	3659.5
18	4258.0
19	4671.5
20	5085.0
21	5991.0
22	6897.0
23	8398.5
24	9900.0
25	14630.5
26	19361.0
27	32941.5
28	46522.0
29	65723.0
30	84924.0
31	112912.0
32	140900.0
33	185782.0
34	230664.0
35	273253.5
36	315843.0
37	302172.5
38	288502.0
39	246319.0
40	204136.0
41	167748.0
42	131360.0
43	105318.0
44	79276.0
45	69120.0
46	58964.0
47	53315.0
48	47666.0
49	42572.0
50	37478.0
51	34459.0
52	31440.0
53	27775.0
54	24110.0
55	21201.5
56	18293.0
57	16124.0
58	13955.0
59	11552.5
60	9150.0
61	7589.0
62	6028.0
63	4874.5
64	3721.0
65	3064.5
66	2408.0
67	1867.0
68	1326.0
69	1077.0
70	828.0
71	709.5
72	591.0
73	483.0
74	375.0
75	303.5
76	232.0
77	166.5
78	101.0
79	73.0
80	45.0
81	33.0
82	21.0
83	15.0
84	9.0
85	8.5
86	8.0
87	6.0
88	4.0
89	5.5
90	7.0
91	4.0
92	1.0
93	1.5
94	2.0
95	3.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0077038782197157
2	6.010117760061892E-4
3	3.82462039276666E-4
4	0.0
5	0.0
6	3.82462039276666E-4
7	5.463743418238084E-5
8	0.0
9	0.001092748683647617
10	0.001092748683647617
11	5.463743418238084E-5
12	2.1854973672952337E-4
13	7.10286644370951E-4
14	3.82462039276666E-4
15	0.004862731642231896
16	7.64924078553332E-4
17	0.0
18	3.82462039276666E-4
19	5.463743418238084E-5
20	6.556492101885701E-4
21	0.0
22	1.0927486836476169E-4
23	6.010117760061892E-4
24	3.2782460509428506E-4
25	0.0020215850647480912
26	0.006064755194244274
27	0.006611129536068083
28	0.004152444997860945
29	0.002841146577483804
30	0.004425632168772849
31	0.008086340258992365
32	0.003387520919307613
33	0.004917369076414276
34	0.003168971182578089
35	0.003551433221854755
36	0.00901517664009284
37	0.003769982958584278
38	0.00901517664009284
39	0.004589544471319991
40	0.003168971182578089
41	0.003769982958584278
42	0.0020215850647480912
43	0.0016391230254714255
44	0.0012566609861947595
45	0.0013659358545595212
46	0.0012566609861947595
47	0.0023494096698423767
48	0.0015844855912890445
49	3.82462039276666E-4
50	0.0012020235520123785
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1830247.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.20802877055728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95006261650877	39.13718986516414
2	12.724487931819478	12.777429123428027
3	3.952378160335891	5.953233491588001
4	1.7412490467127335	3.496987289362335
5	0.902757894610794	2.2662847172733227
6	0.5637478317122105	1.6982800412367574
7	0.375246115673283	1.3188257440234838
8	0.2750263787208055	1.1046825868381107
9	0.19727392725688192	0.8914261513854916
>10	1.11822789213191	10.624392930051982
>50	0.11003643888579981	3.867536989866955
>100	0.07668849236447835	7.667412000355883
>500	0.007887552779747349	2.6423232819483755
>1k	0.004272424422363147	3.978280613239884
>5k	5.477467208157881E-4	1.986142666760113
>10k+	1.0954934416315761E-4	0.5895725074771989
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	10719	0.5856586570009403	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	8857	0.48392375455334713	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	8730	0.4769848004121848	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	7627	0.41671971050901874	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	5762	0.31482089575887845	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	5134	0.2805085870923433	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	3651	0.19948127219987247	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	3498	0.1911217447699682	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	3206	0.175167613988713	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2902	0.15855783399726922	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	2562	0.13998110637525973	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	2555	0.13959864433598307	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	2375	0.1297639061831545	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2339	0.1277969585525888	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	2334	0.1275237713816769	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	2214	0.12096727927979119	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	2163	0.11818077013648978	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT	2002	0.10938414323312647	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATAT	1976	0.10796356994438455	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCAC	1932	0.1055595228403598	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATG	1876	0.10249982652614648	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATT	1873	0.10233591422359933	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG	1861	0.10168026501341076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.463743418238085E-5	0.0
11	0.0	0.0	0.0	5.463743418238085E-5	0.0
12	0.0	0.0	0.0	5.463743418238085E-5	0.0
13	0.0	0.0	0.0	5.463743418238085E-5	0.0
14	0.0	0.0	0.0	5.463743418238085E-5	0.0
15	0.0	0.0	0.0	1.092748683647617E-4	0.0
16	0.0	0.0	0.0	1.092748683647617E-4	0.0
17	0.0	0.0	0.0	1.092748683647617E-4	0.0
18	0.0	0.0	0.0	1.092748683647617E-4	0.0
19	0.0	0.0	0.0	1.092748683647617E-4	0.0
20	0.0	0.0	0.0	1.092748683647617E-4	0.0
21	0.0	0.0	0.0	3.278246050942851E-4	0.0
22	0.0	0.0	0.0	4.370994734590468E-4	0.0
23	0.0	0.0	0.0	0.0010381112494652362	0.0
24	0.0	0.0	0.0	0.0017483978938361872	0.0
25	0.0	0.0	0.0	0.0020762224989304724	0.0
26	0.0	0.0	0.0	0.0024040471040247574	0.0
27	0.0	0.0	0.0	0.0031689711825780894	0.0
28	0.0	0.0	0.0	0.004753456773867134	0.0
29	0.0	0.0	0.0	0.006665766970250463	0.0
30	0.0	0.0	0.0	0.011255311441570454	0.0
31	0.0	0.0	0.0	0.023275546961694243	0.0
32	0.0	0.0	0.0	0.03775446702002517	0.0
33	0.0	0.0	0.0	0.051741650170714665	0.0
34	0.0	0.0	0.0	0.06709476917596369	0.0
35	0.0	0.0	0.0	0.08496121015360222	0.0
36	0.0	0.0	0.0	0.10911095606221455	0.0
37	0.0	0.0	0.0	0.15189206702701877	0.0
38	0.0	0.0	0.0	0.2011203952253439	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	4490	0.0	27.632582	4
TATAGGA	770	0.0	25.140892	2
GACCGGG	80	1.3115823E-7	24.748064	7
GTATCAA	3370	0.0	24.611368	1
GCGAGGG	45	0.0013984578	24.444536	20
ATAGGAC	780	0.0	24.254513	3
TACGACT	65	1.8151499E-5	23.690454	4
GTCCTAT	665	0.0	23.48881	1
AGGACCG	85	2.3558096E-7	23.292295	5
ACTGTTC	1890	0.0	22.347458	8
AGGACCT	8815	0.0	22.310223	5
GGACCTG	8590	0.0	22.22876	6
TCCTATA	720	0.0	21.692747	2
CGGTAGG	155	1.8189894E-12	21.290401	1
CTAGGAC	820	0.0	21.193464	3
GACCTAG	315	0.0	20.950743	7
TGTAGGA	14425	0.0	20.862146	2
TTAGGAC	8665	0.0	20.817759	3
GATATAC	2250	0.0	20.728966	1
CGAGGAG	85	6.1146166E-6	20.706526	21
>>END_MODULE