FastQCFastQC Report
Thu 2 Feb 2017
SRR4063894_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063894_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611726
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23950.14859845904328653No Hit
TATCAACGCAGAGTACTTTTTTTTT16630.10318130997452421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1101.8553692E-1013.8183177
TATAACG551.968852E-413.8110272
CGTTATT905.439415E-712.6601092
GTATCAA17300.012.6274371
CTATACT1750.011.9400084
GGTATCA5950.011.3337821
TAGAACC2200.011.2245944
TAGGACG1701.0913936E-1111.1738044
CGGTCCA2050.011.12240710
GCGTTAT951.3633009E-510.9977181
CCGATAA700.001490558710.85792359
GTTATTC1408.403731E-910.8525313
TCGCACG1605.4205884E-1010.6902618
TAACGAA803.7683302E-410.68826913
CTAGGAC1701.4006218E-1010.6131383
CGCGGTC1355.682159E-810.55598710
AGAACCG1909.094947E-1210.4981475
ATTTGCG1002.3933364E-510.45204816
TATGGGT1002.4048453E-510.4475074
TGTAGGA2750.010.3582712
Produced by FastQC (version 0.11.5)