##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063894_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1611726
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233759336264352	32.0	32.0	32.0	32.0	32.0
2	30.795158110001328	32.0	32.0	32.0	32.0	32.0
3	30.80677546927952	32.0	32.0	32.0	32.0	32.0
4	30.84462681622062	32.0	32.0	32.0	32.0	32.0
5	30.67343146415706	32.0	32.0	32.0	32.0	32.0
6	34.367123816331066	36.0	36.0	36.0	32.0	36.0
7	34.31115772780237	36.0	36.0	36.0	32.0	36.0
8	34.25696241172507	36.0	36.0	36.0	32.0	36.0
9	34.44092544266209	36.0	36.0	36.0	32.0	36.0
10	34.075306224507145	36.0	36.0	36.0	32.0	36.0
11	34.41219847542324	36.0	36.0	36.0	32.0	36.0
12	34.20472276305029	36.0	36.0	36.0	32.0	36.0
13	34.29304857029048	36.0	36.0	36.0	32.0	36.0
14	34.19231618773911	36.0	36.0	36.0	32.0	36.0
15	34.11642177392435	36.0	36.0	36.0	32.0	36.0
16	34.11598869783077	36.0	36.0	36.0	32.0	36.0
17	34.02583503647642	36.0	36.0	36.0	32.0	36.0
18	34.027262078045524	36.0	36.0	36.0	32.0	36.0
19	34.04699185841762	36.0	36.0	36.0	32.0	36.0
20	34.03109399488498	36.0	36.0	36.0	32.0	36.0
21	34.02360016528864	36.0	36.0	36.0	32.0	36.0
22	33.99430176096929	36.0	36.0	36.0	32.0	36.0
23	33.93616222608558	36.0	36.0	36.0	32.0	36.0
24	33.922386931773765	36.0	36.0	36.0	32.0	36.0
25	33.4839687391033	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	15.0
5	24.0
6	65.0
7	29.0
8	78.0
9	116.0
10	138.0
11	42.0
12	97.0
13	83.0
14	148.0
15	260.0
16	402.0
17	593.0
18	771.0
19	1120.0
20	1554.0
21	2203.0
22	3568.0
23	5450.0
24	8387.0
25	12370.0
26	18023.0
27	24195.0
28	34032.0
29	46747.0
30	62798.0
31	85965.0
32	121672.0
33	168871.0
34	344447.0
35	667456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.65761009224994	17.42960216205748	11.792312877624518	26.120474868068065
2	16.64620075538758	19.70398784331075	37.61189806797247	26.0379133333292
3	18.63966986350412	24.00544271038539	28.59110614464133	28.763781281469157
4	12.813776637198012	15.778615287629618	35.96406429163543	35.44354378353694
5	14.72091707186311	36.577821296578975	33.75621445999042	14.945047171567497
6	33.93175387212073	35.359474285146945	17.279338760921366	13.42943308181096
7	30.207705631305593	30.286768935940728	21.076576596144765	18.428948836608917
8	28.01301920730307	32.775246127319605	19.540169244729565	19.671565420647756
9	27.40388614448333	14.52617517322034	18.398104477222812	39.671834205073516
10	15.759188338354587	26.776441052657063	31.96789955916835	25.496471049819995
11	36.53589959114855	21.178393572601863	22.94596530784292	19.33974152840667
12	24.846544448562668	23.30798453120858	29.0241611396593	22.82130988056946
13	29.36527793730075	19.76885897790098	25.250682154708652	25.615180930089622
14	23.674379649100587	19.34634289897129	25.47233713363644	31.506940318291683
15	25.182736421259133	27.15239106277044	22.23492820293148	25.429944313038945
16	25.75775825245612	25.695051506451964	23.638642752838567	24.908547488253348
17	24.228562630414714	25.727575188098296	25.2107899103945	24.833072271092494
18	24.644564477000465	25.28679177566565	25.750998609739028	24.317645137594855
19	25.554738021837466	24.95791771057444	25.3412064934944	24.146137774093702
20	25.8296925877815	24.49282075609944	24.869727956206734	24.807758699912323
21	26.45790723983305	24.35410744820752	24.5367766034465	24.65120870851293
22	25.81943495808755	24.263893200869298	24.786898478733313	25.129773362309844
23	24.741421759629816	24.344387655306836	25.08219798179505	25.8319926032683
24	24.796946608491453	24.79024077062495	25.108581796009656	25.30423082487394
25	24.939784995644153	24.509726609288293	25.1941813584654	25.356307036602153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	109.0
2	254.0
3	399.0
4	399.0
5	399.0
6	975.5
7	1552.0
8	1552.0
9	1552.0
10	1812.5
11	2073.0
12	2073.0
13	2073.0
14	2938.5
15	3804.0
16	3804.0
17	3804.0
18	6452.0
19	9100.0
20	9100.0
21	9100.0
22	15214.5
23	21329.0
24	21329.0
25	21329.0
26	32162.5
27	42996.0
28	42996.0
29	42996.0
30	54935.0
31	66874.0
32	66874.0
33	66874.0
34	84350.5
35	101827.0
36	101827.0
37	101827.0
38	119435.5
39	137044.0
40	137044.0
41	137044.0
42	156441.5
43	175839.0
44	175839.0
45	175839.0
46	193884.5
47	211930.0
48	211930.0
49	211930.0
50	217212.5
51	222495.0
52	222495.0
53	222495.0
54	209463.0
55	196431.0
56	196431.0
57	196431.0
58	178043.5
59	159656.0
60	159656.0
61	159656.0
62	137423.0
63	115190.0
64	115190.0
65	115190.0
66	93392.0
67	71594.0
68	71594.0
69	71594.0
70	53751.5
71	35909.0
72	35909.0
73	35909.0
74	26949.5
75	17990.0
76	17990.0
77	17990.0
78	13883.0
79	9776.0
80	9776.0
81	9776.0
82	6811.5
83	3847.0
84	3847.0
85	3847.0
86	2907.5
87	1968.0
88	1968.0
89	1968.0
90	1410.0
91	852.0
92	852.0
93	852.0
94	590.5
95	329.0
96	329.0
97	329.0
98	571.0
99	813.0
100	813.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04144625078952626
2	0.006390664418145516
3	0.0017993132827788345
4	0.004343169992914429
5	0.010051336269316247
6	0.012781328836291033
7	0.02227425753508971
8	0.03524172222821993
9	0.047898960493284834
10	0.056275074051048375
11	0.05410348905459117
12	0.061983240327450194
13	0.0633482366109376
14	0.06607822917791237
15	0.058074387333827214
16	0.06539573103616868
17	0.061983240327450194
18	0.07675001830335926
19	0.07389593516515834
20	0.07755660701632908
21	0.07222071245360563
22	0.07606752016161555
23	0.07972819201278629
24	0.07377184459393223
25	0.07737047115948989
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1611726.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.6496648256924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.42045074794585	47.970529322460735
2	11.583686196289039	13.819259981092818
3	3.3380235623754646	5.973359600278805
4	1.4606925286716324	3.485192789946238
5	0.7924881630038824	2.363582665075513
6	0.509813022808594	1.824610556058342
7	0.3523924418591385	1.4714063730803437
8	0.23795133304788768	1.135497380890656
9	0.18223557257238995	0.9783261740935082
>10	1.007067484853582	10.541878613241309
>50	0.061160146359325054	2.5134834233569854
>100	0.04933544327185144	5.7917251349630945
>500	0.004285280768884234	1.7485351315056692
>1k	4.180761725740716E-4	0.38261285395601125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2395	0.14859845904328653	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1663	0.10318130997452421	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.204528561306326E-5
2	0.0	0.0	0.0	0.0	6.204528561306326E-5
3	0.0	0.0	0.0	0.0	6.204528561306326E-5
4	0.0	0.0	0.0	0.0	6.204528561306326E-5
5	0.0	0.0	0.0	0.0	1.2409057122612653E-4
6	0.0	0.0	0.0	0.0	1.8613585683918978E-4
7	0.0	0.0	0.0	0.0	1.8613585683918978E-4
8	0.0	0.0	0.0	0.0	1.8613585683918978E-4
9	0.0	0.0	0.0	0.0	1.8613585683918978E-4
10	0.0	0.0	0.0	0.0	2.4818114245225305E-4
11	0.0	0.0	0.0	0.0	2.4818114245225305E-4
12	0.0	0.0	0.0	0.0	4.963622849045061E-4
13	0.0	0.0	0.0	0.0	6.204528561306326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	110	1.8553692E-10	13.818317	7
TATAACG	55	1.968852E-4	13.811027	2
CGTTATT	90	5.439415E-7	12.660109	2
GTATCAA	1730	0.0	12.627437	1
CTATACT	175	0.0	11.940008	4
GGTATCA	595	0.0	11.333782	1
TAGAACC	220	0.0	11.224594	4
TAGGACG	170	1.0913936E-11	11.173804	4
CGGTCCA	205	0.0	11.122407	10
GCGTTAT	95	1.3633009E-5	10.997718	1
CCGATAA	70	0.0014905587	10.8579235	9
GTTATTC	140	8.403731E-9	10.852531	3
TCGCACG	160	5.4205884E-10	10.69026	18
TAACGAA	80	3.7683302E-4	10.688269	13
CTAGGAC	170	1.4006218E-10	10.613138	3
CGCGGTC	135	5.682159E-8	10.555987	10
AGAACCG	190	9.094947E-12	10.498147	5
ATTTGCG	100	2.3933364E-5	10.452048	16
TATGGGT	100	2.4048453E-5	10.447507	4
TGTAGGA	275	0.0	10.358271	2
>>END_MODULE