FastQCFastQC Report
Thu 2 Feb 2017
SRR4063894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611726
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18550.023.718841
GCGAATT500.002580871321.99990713
GGTATCA9000.019.5548651
TAGTACG750.0012911617.5999264
CGTCTTA1451.2500459E-716.68958515
GTTATAT1854.2382453E-1016.6480621
TCTAGAT2800.016.4983942
CGAGCCG4150.016.43366615
TTAGTAC953.3480785E-416.2099553
GTCCTAT4650.015.6123521
TGTCGAC1158.220537E-515.30428412
GTGTCGA1452.2867043E-615.1723511
GTACTAA1452.2874428E-615.171881
AACGCAG27750.015.1422796
TCAACGC28400.014.8731774
TTAACGG1351.9545583E-514.66842535
CCGTCGT2551.2732926E-1114.6679719
GCCCGTT1057.1592024E-414.6670627
GGCGTAA900.00435996714.6666056
ATAGCGC900.00435996714.6666058
Produced by FastQC (version 0.11.5)