##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1611726
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227170747385102	32.0	32.0	32.0	32.0	32.0
2	31.21769395046056	32.0	32.0	32.0	32.0	32.0
3	31.36682599896012	32.0	32.0	32.0	32.0	32.0
4	31.486865013035715	32.0	32.0	32.0	32.0	32.0
5	31.38442142150713	32.0	32.0	32.0	32.0	32.0
6	34.99620655123762	36.0	36.0	36.0	36.0	36.0
7	35.014815793751545	36.0	36.0	36.0	36.0	36.0
8	34.94437143782504	36.0	36.0	36.0	36.0	36.0
9	35.10929773423026	36.0	36.0	36.0	36.0	36.0
10	34.89340123569391	36.0	36.0	36.0	32.0	36.0
11	35.10977424202377	36.0	36.0	36.0	36.0	36.0
12	34.994508992223246	36.0	36.0	36.0	36.0	36.0
13	35.05788142649557	36.0	36.0	36.0	36.0	36.0
14	34.99164063866935	36.0	36.0	36.0	36.0	36.0
15	34.94718270971617	36.0	36.0	36.0	36.0	36.0
16	34.97772698337062	36.0	36.0	36.0	36.0	36.0
17	34.93413892932173	36.0	36.0	36.0	36.0	36.0
18	34.93870794415428	36.0	36.0	36.0	36.0	36.0
19	34.932640535674174	36.0	36.0	36.0	36.0	36.0
20	34.93410108169751	36.0	36.0	36.0	36.0	36.0
21	34.931880480925415	36.0	36.0	36.0	36.0	36.0
22	34.91047113467177	36.0	36.0	36.0	32.0	36.0
23	34.852556824174826	36.0	36.0	36.0	32.0	36.0
24	34.82903359504035	36.0	36.0	36.0	32.0	36.0
25	34.80249868774221	36.0	36.0	36.0	32.0	36.0
26	34.745503888378046	36.0	36.0	36.0	32.0	36.0
27	34.736498635624166	36.0	36.0	36.0	32.0	36.0
28	34.72119144321057	36.0	36.0	36.0	32.0	36.0
29	34.692502323595946	36.0	36.0	36.0	32.0	36.0
30	34.677880111135515	36.0	36.0	36.0	32.0	36.0
31	34.66903617612423	36.0	36.0	36.0	32.0	36.0
32	34.62167887097435	36.0	36.0	36.0	32.0	36.0
33	34.606510039547665	36.0	36.0	36.0	32.0	36.0
34	34.60136090129464	36.0	36.0	36.0	32.0	36.0
35	34.561868456549064	36.0	36.0	36.0	32.0	36.0
36	34.54507900226217	36.0	36.0	36.0	32.0	36.0
37	34.534793755266094	36.0	36.0	36.0	32.0	36.0
38	34.50182785411416	36.0	36.0	36.0	32.0	36.0
39	34.494221722550854	36.0	36.0	36.0	32.0	36.0
40	34.485951085978634	36.0	36.0	36.0	32.0	36.0
41	34.47103167660012	36.0	36.0	36.0	32.0	36.0
42	34.4109600515224	36.0	36.0	36.0	32.0	36.0
43	34.40053582308656	36.0	36.0	36.0	32.0	36.0
44	34.34816587931199	36.0	36.0	36.0	32.0	36.0
45	34.31806957261966	36.0	36.0	36.0	32.0	36.0
46	34.30906804258292	36.0	36.0	36.0	32.0	36.0
47	34.27899779491055	36.0	36.0	36.0	32.0	36.0
48	34.24149886519172	36.0	36.0	36.0	32.0	36.0
49	34.230131548414555	36.0	36.0	36.0	32.0	36.0
50	33.595611164676875	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	4.0
20	14.0
21	46.0
22	140.0
23	432.0
24	1115.0
25	2760.0
26	5711.0
27	10533.0
28	17489.0
29	27804.0
30	41501.0
31	61055.0
32	91609.0
33	151303.0
34	313571.0
35	886634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58770209829783	18.55786079851476	12.47586237707807	26.37857472610934
2	15.88794324568985	20.685739883937586	36.98001946989519	26.44629740047738
3	17.896076370490693	24.72188131700132	28.9115616713056	28.47048064120239
4	12.503924364315026	16.57049647396642	36.193869181238	34.73170998048055
5	14.447182709716166	37.04202823556857	33.36938164427452	15.141407410440733
6	34.0379829536465	35.746341027583526	16.949562672990336	13.26611334577964
7	30.08482831449018	30.647020647430146	20.985887179334455	18.28226385874522
8	28.03511765344584	33.07865795963952	19.313344398082798	19.572879988831843
9	27.47296656714492	14.336716080083987	18.210556750974742	39.97976060179636
10	15.58046638836908	27.085866883745403	32.00457404089088	25.329092686994635
11	36.82735154734738	21.136843359231037	22.858537989707926	19.177267103713657
12	24.865128809354957	23.464577970283976	28.908379417555004	22.761913802806067
13	29.502504786829427	19.758716496847455	25.161349966557346	25.57742874976578
14	23.67792272254882	19.26525927189812	25.413206347236656	31.643611658316402
15	25.211864143862854	27.171537670183156	22.192032245294563	25.424565940659427
16	25.815888600237386	25.779901955005524	23.574603107632957	24.829606337124133
17	24.231863376196312	25.92135755169151	25.114271975678232	24.732507096433945
18	24.77907183802676	25.256325063301823	25.762615536292525	24.201987562378896
19	25.682452878069	24.97085417283243	25.228528723608214	24.11816422549036
20	25.999429181247365	24.4255205618842	24.788486833941377	24.78656342292706
21	26.533542301855277	24.38330088364896	24.49008082018904	24.593075994306723
22	25.77413998922892	24.359381631098127	24.78836326815261	25.078115111520333
23	24.790533816131376	24.242050088228943	25.139726850139848	25.82768924549983
24	24.960228823865187	24.81293276747822	25.006328642692278	25.220509765964312
25	25.043106523836556	24.42072215448857	25.188790752650142	25.34738056902473
26	25.070906341580248	24.92614633728647	25.21665912772945	24.786288193403834
27	25.275682598303835	24.94353563707934	24.635277444782815	25.145504319834007
28	24.874570474454323	24.715790283570623	25.215833096517752	25.193806145457298
29	24.96883688521488	24.826314884893474	25.06308630554061	25.141761924351037
30	24.989482906465497	24.868489795006646	25.126608432785535	25.015418865742323
31	25.438022262085518	24.60147218418249	24.63913617300367	25.321369380728324
32	25.12203188123616	24.809126439734214	24.559509427804343	25.50933225122528
33	24.864377532741432	24.527888620494736	25.00051189996792	25.60722194679591
34	25.360385933888658	24.49172444816728	25.132734577380678	25.015155040563393
35	25.695270879842948	24.526734314755963	24.98383668161179	24.794158123789302
36	25.081487392335188	24.849729988663434	24.821683309557773	25.247099309443605
37	25.631273019686486	24.613753238888503	24.734311866493265	25.020661874931747
38	25.10899174107558	24.604955354649753	24.98011280784815	25.30594009642651
39	25.376135791101305	24.633047332798892	24.683120177308748	25.307696698791055
40	25.643116144355844	24.731150771491187	24.884654808262663	24.74107827589031
41	24.926147803668876	24.877440411003526	25.143128379657064	25.053283405670534
42	25.748042293047064	24.99951914336787	24.71907114630637	24.533367417278697
43	25.20147842882502	24.361935855745852	25.086011077668392	25.350574637760744
44	25.115979517368853	24.64436774177937	24.729308739119453	25.510344001732328
45	25.071880355350167	24.900571195987357	24.806943698168276	25.220604750494196
46	25.10381849802725	24.72788168072733	24.75896673526888	25.40933308597654
47	25.166842878557745	24.759507996545256	25.02792089823391	25.045728226663094
48	25.567367107629902	25.027874311518467	24.514689157218562	24.890069423633072
49	25.183019103229658	24.855852485268816	24.71606367619129	25.24506473531023
50	25.162095320114215	25.129645233063595	24.61944051830793	25.088818928514257
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	26.5
2	23.0
3	68.5
4	114.0
5	141.5
6	169.0
7	160.5
8	152.0
9	204.5
10	257.0
11	404.5
12	552.0
13	1046.0
14	1540.0
15	2007.0
16	2474.0
17	2497.5
18	2521.0
19	2621.0
20	2721.0
21	3105.0
22	3489.0
23	4351.0
24	5213.0
25	6591.5
26	7970.0
27	10359.0
28	12748.0
29	15379.5
30	18011.0
31	21122.0
32	24233.0
33	28506.5
34	32780.0
35	38321.0
36	43862.0
37	51097.0
38	58332.0
39	64736.0
40	71140.0
41	78425.5
42	85711.0
43	89561.5
44	93412.0
45	101325.0
46	109238.0
47	115759.5
48	122281.0
49	125444.0
50	128607.0
51	127177.0
52	125747.0
53	124364.5
54	122982.0
55	120730.0
56	118478.0
57	113246.0
58	108014.0
59	98418.0
60	88822.0
61	78372.5
62	67923.0
63	58590.5
64	49258.0
65	41726.5
66	34195.0
67	28872.0
68	23549.0
69	20561.0
70	17573.0
71	13728.5
72	9884.0
73	8385.5
74	6887.0
75	5329.5
76	3772.0
77	3313.0
78	2854.0
79	2304.0
80	1754.0
81	1435.5
82	1117.0
83	892.0
84	667.0
85	494.0
86	321.0
87	230.5
88	140.0
89	100.0
90	60.0
91	53.0
92	46.0
93	40.5
94	35.0
95	32.5
96	30.0
97	27.0
98	24.0
99	19.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008314068272150477
2	4.3431699929144285E-4
3	2.4818114245225305E-4
4	0.0
5	0.0
6	1.861358568391898E-4
7	0.0
8	6.204528561306326E-5
9	0.0011168151410351388
10	0.0013649962834873918
11	0.0
12	1.861358568391898E-4
13	7.445434273567592E-4
14	6.204528561306326E-5
15	0.004963622849045061
16	0.0010547698554220753
17	6.204528561306326E-5
18	1.861358568391898E-4
19	1.861358568391898E-4
20	3.722717136783796E-4
21	0.0
22	1.2409057122612653E-4
23	6.204528561306326E-4
24	3.722717136783796E-4
25	0.0013029509978743285
26	0.005770211562014883
27	0.005584075705175694
28	0.003970898279236049
29	0.002792037852587847
30	0.003970898279236049
31	0.00663884556059777
32	0.003536581279944606
33	0.005149758705884251
34	0.0031643095662662263
35	0.00322635485187929
36	0.007879751272859035
37	0.0038468077080099227
38	0.008438158843376602
39	0.004591351135366681
40	0.002605901995748657
41	0.003784762422396859
42	0.0011788604266482021
43	9.306792841959489E-4
44	0.0011788604266482021
45	0.0012409057122612652
46	0.0011788604266482021
47	0.0018613585683918978
48	0.0016752227115527081
49	5.584075705175694E-4
50	0.0012409057122612652
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1611726.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.365676949282324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14456108596363	50.60635482878513
2	11.427934122696866	14.25421695387586
3	3.1530260173336924	5.899218060291458
4	1.3762595844353986	3.433254425650064
5	0.7486116920863383	2.3343837474556093
6	0.4412485367710826	1.6511258219165315
7	0.3207333528808376	1.400192687083854
8	0.22821837278073323	1.1386394648587275
9	0.1699545090590061	0.9539395207243065
>10	0.8944598976018462	9.735908481696358
>50	0.05099901410122635	2.1847306343951063
>100	0.04059449234719311	4.938307166429421
>500	0.003199361827979837	1.3411558271126467
>1k	1.999601142487398E-4	0.12857237972505822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.204528561306326E-5	0.0
8	0.0	0.0	0.0	6.204528561306326E-5	0.0
9	0.0	0.0	0.0	6.204528561306326E-5	0.0
10	0.0	0.0	0.0	6.204528561306326E-5	0.0
11	0.0	0.0	0.0	6.204528561306326E-5	0.0
12	0.0	0.0	0.0	6.204528561306326E-5	1.8613585683918978E-4
13	0.0	0.0	0.0	6.204528561306326E-5	1.8613585683918978E-4
14	0.0	0.0	0.0	6.204528561306326E-5	1.8613585683918978E-4
15	0.0	0.0	0.0	1.2409057122612653E-4	3.102264280653163E-4
16	0.0	0.0	0.0	1.2409057122612653E-4	3.102264280653163E-4
17	0.0	0.0	0.0	1.8613585683918978E-4	3.102264280653163E-4
18	0.0	0.0	0.0	1.8613585683918978E-4	3.102264280653163E-4
19	0.0	0.0	0.0	2.4818114245225305E-4	3.102264280653163E-4
20	0.0	0.0	0.0	2.4818114245225305E-4	3.102264280653163E-4
21	0.0	0.0	0.0	2.4818114245225305E-4	3.102264280653163E-4
22	0.0	0.0	0.0	4.3431699929144285E-4	3.102264280653163E-4
23	0.0	0.0	0.0	6.204528561306326E-4	3.7227171367837955E-4
24	0.0	0.0	0.0	7.445434273567591E-4	3.7227171367837955E-4
25	0.0	0.0	0.0	0.001178860426648202	3.7227171367837955E-4
26	0.0	0.0	0.0	0.0013649962834873918	4.963622849045061E-4
27	0.0	0.0	0.0	0.0016752227115527081	4.963622849045061E-4
28	0.0	0.0	0.0	0.0026679472813617203	5.584075705175694E-4
29	0.0	0.0	0.0	0.003288400137492353	5.584075705175694E-4
30	0.0	0.0	0.0	0.006824981417436959	5.584075705175694E-4
31	0.0	0.0	0.0	0.018241313970240598	5.584075705175694E-4
32	0.0	0.0	0.0	0.03021605409356181	5.584075705175694E-4
33	0.0	0.0	0.0	0.03995716393481274	5.584075705175694E-4
34	0.0	0.0	0.0	0.05385530791213891	6.204528561306326E-4
35	0.0	0.0	0.0	0.06887026703050023	6.204528561306326E-4
36	0.0	0.0	0.0	0.09114452456558993	6.204528561306326E-4
37	0.0	0.0	0.0	0.12880601293271934	6.204528561306326E-4
38	0.0	0.0	0.0	0.17571224885619516	6.204528561306326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1855	0.0	23.71884	1
GCGAATT	50	0.0025808713	21.999907	13
GGTATCA	900	0.0	19.554865	1
TAGTACG	75	0.00129116	17.599926	4
CGTCTTA	145	1.2500459E-7	16.689585	15
GTTATAT	185	4.2382453E-10	16.648062	1
TCTAGAT	280	0.0	16.498394	2
CGAGCCG	415	0.0	16.433666	15
TTAGTAC	95	3.3480785E-4	16.209955	3
GTCCTAT	465	0.0	15.612352	1
TGTCGAC	115	8.220537E-5	15.304284	12
GTGTCGA	145	2.2867043E-6	15.17235	11
GTACTAA	145	2.2874428E-6	15.17188	1
AACGCAG	2775	0.0	15.142279	6
TCAACGC	2840	0.0	14.873177	4
TTAACGG	135	1.9545583E-5	14.668425	35
CCGTCGT	255	1.2732926E-11	14.667971	9
GCCCGTT	105	7.1592024E-4	14.66706	27
GGCGTAA	90	0.004359967	14.666605	6
ATAGCGC	90	0.004359967	14.666605	8
>>END_MODULE