FastQCFastQC Report
Thu 2 Feb 2017
SRR4063893_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063893_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457703
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT32750.22466853673210524No Hit
GTCCTACAGTGGACATTTCTAAATT31990.219454854658322No Hit
CTTTAGGACGTGAAATATGGCGAGG27330.1874867514164408No Hit
GTATCAACGCAGAGTACTTTTTTTT27030.1854287190188948No Hit
CTGTAGGACGTGGAATATGGCAAGA26390.18103824990413No Hit
TATCAACGCAGAGTACTTTTTTTTT20360.13967179871345536No Hit
GTCCTACAGTGTGCATTTCTCATTT18660.12800961512736134No Hit
CTGTAGGACCTGGAATATGGCGAGA15240.10454804579533691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT851.022272E-915.6430471
AACCGCG1006.5847416E-1014.2507477
CGCGGTC1153.765308E-1013.21989810
CGACCCG1700.012.8509695
TCCCGAC1850.012.8327852
CGGTCCA1650.012.66906910
GAACCGC1103.8171493E-812.0898826
TCTATAC951.0441363E-611.9956883
ATCCCCG2000.011.8776612
TAGACAG1603.6379788E-1211.8731785
GTACTAA802.873605E-511.8719551
TTAGGAC10600.011.8259373
TTTAGGA11200.011.7855382
TTTTCGG1701.8189894E-1211.73590916
GCCGCCT2950.011.5934318
AGAACCG1157.086237E-811.5634425
CTCCCGA2300.011.5622511
CGGTTCT2150.011.49094612
CAAATCG1251.8284481E-811.40098913
CGCCGGT1850.011.297897
Produced by FastQC (version 0.11.5)