##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063893_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1457703
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26850325477824	32.0	32.0	32.0	32.0	32.0
2	30.96057290133861	32.0	32.0	32.0	32.0	32.0
3	30.975279600851476	32.0	32.0	32.0	32.0	32.0
4	30.984607975698754	32.0	32.0	32.0	32.0	32.0
5	30.938110163730197	32.0	32.0	32.0	32.0	32.0
6	34.43977751297761	36.0	36.0	36.0	32.0	36.0
7	34.411266218152804	36.0	36.0	36.0	32.0	36.0
8	34.36440756450388	36.0	36.0	36.0	32.0	36.0
9	34.52011212160502	36.0	36.0	36.0	32.0	36.0
10	34.25693779871483	36.0	36.0	36.0	32.0	36.0
11	34.46445606546738	36.0	36.0	36.0	32.0	36.0
12	34.316620738243664	36.0	36.0	36.0	32.0	36.0
13	34.39218414176276	36.0	36.0	36.0	32.0	36.0
14	34.30439396776984	36.0	36.0	36.0	32.0	36.0
15	34.23504582209133	36.0	36.0	36.0	32.0	36.0
16	34.261906574933306	36.0	36.0	36.0	32.0	36.0
17	34.171261223994186	36.0	36.0	36.0	32.0	36.0
18	34.178232465735476	36.0	36.0	36.0	32.0	36.0
19	34.162550258866176	36.0	36.0	36.0	32.0	36.0
20	34.12025494905341	36.0	36.0	36.0	32.0	36.0
21	34.07989007362954	36.0	36.0	36.0	32.0	36.0
22	34.05745203240989	36.0	36.0	36.0	32.0	36.0
23	34.00856758887098	36.0	36.0	36.0	32.0	36.0
24	33.98191195325797	36.0	36.0	36.0	32.0	36.0
25	33.39425520836549	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	11.0
5	14.0
6	57.0
7	20.0
8	75.0
9	87.0
10	128.0
11	49.0
12	89.0
13	77.0
14	178.0
15	397.0
16	707.0
17	903.0
18	1144.0
19	1305.0
20	1788.0
21	2279.0
22	3177.0
23	4372.0
24	6107.0
25	8634.0
26	12526.0
27	17052.0
28	23922.0
29	34348.0
30	48384.0
31	72340.0
32	112133.0
33	173159.0
34	377701.0
35	554536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.48521565239233	17.91936908404778	11.91689719136169	25.67851807219821
2	15.410732649710798	19.855765569655336	40.12967652218297	24.603825258450893
3	18.635987884899688	24.689147518153785	28.778576990227656	27.89628760671887
4	11.768882632238626	16.098868794888208	37.163542114734064	34.968706458139096
5	13.527045652724256	37.84195978158482	34.4753089172477	14.15568564844322
6	33.124851202625834	36.49338361969895	17.62728307486901	12.754482102806207
7	29.560529968731323	30.67232801027056	21.284314404931077	18.482827616067034
8	26.960840826992545	35.28161610505396	19.3472281695708	18.410314898382687
9	27.240414206219132	14.350277134927236	18.924650595860683	39.48465806299295
10	15.587658529118839	27.31227550931458	32.663055825724186	24.437010135842392
11	36.337142461009	20.708897215326104	24.094213965032164	18.859746358632723
12	25.19985228160398	23.467593247237488	30.278358568559323	21.05419590259921
13	29.990156426329705	20.315378764789386	25.293316529241043	24.401148279639866
14	22.52883978431514	21.185648925179596	25.704390923662523	30.581120366842747
15	23.97964121589852	30.13772724308704	22.227865999938224	23.654765541076213
16	24.205988550089923	25.91153091063853	26.45162618926674	23.430854350004804
17	22.720130804824457	27.198219143384904	26.611876358689123	23.469773693101516
18	23.341180412098645	25.29416247366286	28.89001938084283	22.47463773339567
19	25.356197919312606	25.01177371941064	25.691354176821356	23.940674184455396
20	25.408698207302606	25.233355485741903	26.115218155454983	23.242728151500504
21	25.65227897755279	24.694129454876972	24.662893656867553	24.99069791070269
22	25.181434158846454	26.254077162466555	25.19180084430131	23.372687834385676
23	23.011778695637847	26.2414454099063	26.275155712763503	24.47162018169235
24	24.551854814072353	25.595713349100485	26.406565907944103	23.445865928883062
25	23.839790194907284	25.840627767594178	26.458440604425405	23.861141433073136
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	114.0
1	114.0
2	279.0
3	444.0
4	444.0
5	444.0
6	1325.5
7	2207.0
8	2207.0
9	2207.0
10	2274.0
11	2341.0
12	2341.0
13	2341.0
14	2981.5
15	3622.0
16	3622.0
17	3622.0
18	6725.5
19	9829.0
20	9829.0
21	9829.0
22	17430.5
23	25032.0
24	25032.0
25	25032.0
26	39980.5
27	54929.0
28	54929.0
29	54929.0
30	72347.5
31	89766.0
32	89766.0
33	89766.0
34	103853.5
35	117941.0
36	117941.0
37	117941.0
38	127117.0
39	136293.0
40	136293.0
41	136293.0
42	148959.5
43	161626.0
44	161626.0
45	161626.0
46	177496.0
47	193366.0
48	193366.0
49	193366.0
50	188530.0
51	183694.0
52	183694.0
53	183694.0
54	166517.5
55	149341.0
56	149341.0
57	149341.0
58	134836.5
59	120332.0
60	120332.0
61	120332.0
62	105007.0
63	89682.0
64	89682.0
65	89682.0
66	73207.5
67	56733.0
68	56733.0
69	56733.0
70	42625.5
71	28518.0
72	28518.0
73	28518.0
74	21902.0
75	15286.0
76	15286.0
77	15286.0
78	12490.5
79	9695.0
80	9695.0
81	9695.0
82	6559.5
83	3424.0
84	3424.0
85	3424.0
86	2646.0
87	1868.0
88	1868.0
89	1868.0
90	1307.0
91	746.0
92	746.0
93	746.0
94	493.0
95	240.0
96	240.0
97	240.0
98	437.0
99	634.0
100	634.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03567256155746404
2	0.005213682073783206
3	0.0012348194385276012
4	0.0037044583155828037
5	0.0076833209508384084
6	0.012622598704948813
7	0.022912760692678825
8	0.032311108641472235
9	0.04465930302674825
10	0.05446925745505086
11	0.052411225057504854
12	0.06016314708826146
13	0.06276998812515307
14	0.06503382376245367
15	0.057487704971451656
16	0.06331879676449867
17	0.060094546008343255
18	0.07614719870920207
19	0.07333455443255588
20	0.07731341706781149
21	0.07141372419484628
22	0.07642160302887488
23	0.08060626890388509
24	0.07463797495100168
25	0.07635300194895668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1457703.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.573183674616914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.60445308081263	37.72984708600278
2	14.808208065485069	14.9779645277544
3	4.878298890538316	7.401333174326225
4	2.1515234052825174	4.352375534223601
5	1.0825462490900561	2.73739051457495
6	0.606543579647588	1.840490391610626
7	0.37138451483193047	1.31474680977526
8	0.24283511521127704	0.982475590738134
9	0.18058821525866847	0.8219628881773109
>10	0.8716966904999356	8.131370138972072
>50	0.09453095907199026	3.362560677826982
>100	0.09284589639552783	9.749389384051014
>500	0.010875018971626224	3.6692204703842437
>1k	0.003670318902923851	2.928872811582476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	3275	0.22466853673210524	No Hit
GTCCTACAGTGGACATTTCTAAATT	3199	0.219454854658322	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2733	0.1874867514164408	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2703	0.1854287190188948	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2639	0.18103824990413	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2036	0.13967179871345536	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1866	0.12800961512736134	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1524	0.10454804579533691	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.860107991820007E-5
7	0.0	0.0	0.0	6.860107991820007E-5	6.860107991820007E-5
8	0.0	0.0	0.0	6.860107991820007E-5	6.860107991820007E-5
9	0.0	0.0	0.0	6.860107991820007E-5	6.860107991820007E-5
10	0.0	0.0	0.0	6.860107991820007E-5	6.860107991820007E-5
11	0.0	0.0	0.0	6.860107991820007E-5	6.860107991820007E-5
12	0.0	0.0	0.0	6.860107991820007E-5	4.1160647950920043E-4
13	0.0	0.0	0.0	6.860107991820007E-5	4.8020755942740053E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	85	1.022272E-9	15.643047	1
AACCGCG	100	6.5847416E-10	14.250747	7
CGCGGTC	115	3.765308E-10	13.219898	10
CGACCCG	170	0.0	12.850969	5
TCCCGAC	185	0.0	12.832785	2
CGGTCCA	165	0.0	12.669069	10
GAACCGC	110	3.8171493E-8	12.089882	6
TCTATAC	95	1.0441363E-6	11.995688	3
ATCCCCG	200	0.0	11.87766	12
TAGACAG	160	3.6379788E-12	11.873178	5
GTACTAA	80	2.873605E-5	11.871955	1
TTAGGAC	1060	0.0	11.825937	3
TTTAGGA	1120	0.0	11.785538	2
TTTTCGG	170	1.8189894E-12	11.735909	16
GCCGCCT	295	0.0	11.59343	18
AGAACCG	115	7.086237E-8	11.563442	5
CTCCCGA	230	0.0	11.562251	1
CGGTTCT	215	0.0	11.490946	12
CAAATCG	125	1.8284481E-8	11.400989	13
CGCCGGT	185	0.0	11.29789	7
>>END_MODULE