FastQCFastQC Report
Thu 2 Feb 2017
SRR4063893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457703
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA14760.1012551939592633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA23750.020.3774852
GCATTCG4250.020.18747922
TTAGGAC14300.019.9992523
CTGTAGG22850.019.6404041
GTCCTAA12100.019.4538171
GATATAC6500.019.2915861
TAGGACG22700.019.0917974
GTAGGAC24900.018.9069233
TAGGACC14150.018.8120174
AGGACCT15750.018.5770845
AGGACGT22950.018.5003975
GGACGTG22900.018.4447256
TAGGACA8350.018.4424234
TTCGTAT4900.018.40747525
GGCGAGA14700.018.40747519
GCGAGAA14600.018.38287420
CGCCTAA600.007414414518.33264739
TTTAGGA15900.018.262842
TATGGCG26350.018.11634816
TGGCGAG26750.018.09216118
Produced by FastQC (version 0.11.5)