##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1457703
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14840540219784	32.0	32.0	32.0	32.0	32.0
2	31.020443121815624	32.0	32.0	32.0	32.0	32.0
3	31.275869638739852	32.0	32.0	32.0	32.0	32.0
4	31.4307283445256	32.0	32.0	32.0	32.0	32.0
5	31.29292935529391	32.0	32.0	32.0	32.0	32.0
6	34.9063197372853	36.0	36.0	36.0	36.0	36.0
7	34.946969307190834	36.0	36.0	36.0	36.0	36.0
8	34.86033574740534	36.0	36.0	36.0	32.0	36.0
9	35.04122101690125	36.0	36.0	36.0	36.0	36.0
10	34.798587915370966	36.0	36.0	36.0	32.0	36.0
11	35.05326325047009	36.0	36.0	36.0	36.0	36.0
12	34.925816850208854	36.0	36.0	36.0	32.0	36.0
13	35.00349865507583	36.0	36.0	36.0	36.0	36.0
14	34.91583402105916	36.0	36.0	36.0	32.0	36.0
15	34.856085910504405	36.0	36.0	36.0	32.0	36.0
16	34.89430563015923	36.0	36.0	36.0	32.0	36.0
17	34.85469948267926	36.0	36.0	36.0	32.0	36.0
18	34.853424188603576	36.0	36.0	36.0	32.0	36.0
19	34.85666558962971	36.0	36.0	36.0	32.0	36.0
20	34.83733654935196	36.0	36.0	36.0	32.0	36.0
21	34.828124796340546	36.0	36.0	36.0	32.0	36.0
22	34.801429372101175	36.0	36.0	36.0	32.0	36.0
23	34.75084636582349	36.0	36.0	36.0	32.0	36.0
24	34.719184223398045	36.0	36.0	36.0	32.0	36.0
25	34.676696830561504	36.0	36.0	36.0	32.0	36.0
26	34.614962718743115	36.0	36.0	36.0	32.0	36.0
27	34.5853757589852	36.0	36.0	36.0	32.0	36.0
28	34.540051711494044	36.0	36.0	36.0	32.0	36.0
29	34.50189441882194	36.0	36.0	36.0	32.0	36.0
30	34.47421799913974	36.0	36.0	36.0	32.0	36.0
31	34.471903398703304	36.0	36.0	36.0	32.0	36.0
32	34.41539943321788	36.0	36.0	36.0	32.0	36.0
33	34.414871890913304	36.0	36.0	36.0	32.0	36.0
34	34.39101792340415	36.0	36.0	36.0	32.0	36.0
35	34.356261186263595	36.0	36.0	36.0	32.0	36.0
36	34.3011580548301	36.0	36.0	36.0	32.0	36.0
37	34.260732124445106	36.0	36.0	36.0	32.0	36.0
38	34.20223255354486	36.0	36.0	36.0	32.0	36.0
39	34.14942412823463	36.0	36.0	36.0	32.0	36.0
40	34.1589212617385	36.0	36.0	36.0	32.0	36.0
41	34.133324826799424	36.0	36.0	36.0	32.0	36.0
42	34.06626109708219	36.0	36.0	36.0	32.0	36.0
43	34.06871495771087	36.0	36.0	36.0	32.0	36.0
44	33.96171373729765	36.0	36.0	36.0	32.0	36.0
45	33.920516730774374	36.0	36.0	36.0	32.0	36.0
46	33.88705655404427	36.0	36.0	36.0	32.0	36.0
47	33.85374455564679	36.0	36.0	36.0	32.0	36.0
48	33.820314563391854	36.0	36.0	36.0	32.0	36.0
49	33.79215930817183	36.0	36.0	36.0	32.0	36.0
50	33.116134082182725	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	18.0
21	47.0
22	156.0
23	451.0
24	1110.0
25	2677.0
26	5507.0
27	10656.0
28	18155.0
29	29017.0
30	43382.0
31	65150.0
32	98894.0
33	164242.0
34	327532.0
35	690705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.179187951684895	19.054979493660117	12.61795090278664	26.14788165186835
2	14.867747045171878	20.747424536889987	39.34008324077879	25.044745177159346
3	18.03617085353363	25.3236449650235	29.218086387188524	27.422097794254345
4	11.633576935768122	16.88684183266413	37.399456542244884	34.08012468932286
5	13.557014014514618	38.023794970580425	33.9340043890971	14.485186625807863
6	33.420205227131476	36.95309466543916	17.093411410562652	12.533288696866713
7	29.5661175592492	30.75566354919637	21.268451168588303	18.409767722966127
8	27.033558962285188	35.35830001035876	19.040778539935776	18.567362487420276
9	27.162833071387947	14.118021545150764	18.71723824727324	40.001907136188045
10	15.552198648410398	27.467755511970644	32.46106342153447	24.51898241808449
11	36.662637493808745	20.55406386216113	23.97280102517524	18.810497618854882
12	25.24583213853037	23.500002058033807	29.99440214803849	21.259763655397332
13	30.09788728384568	20.342499161348346	25.241185239456488	24.318428315349486
14	22.591068121012555	21.04150373876655	25.660355354325308	30.70707278589559
15	24.101284545661606	30.092286414440917	22.093375849663428	23.713053190234053
16	24.325064108104993	25.94392238700303	26.121874687005644	23.609138817886333
17	22.891288554664428	27.299045141568616	26.278809881025143	23.530856422741806
18	23.638644935659002	25.161452752345813	28.736600484872767	22.463301827122418
19	25.611801580980487	24.947605925212475	25.688497588329035	23.752094905478003
20	25.80093915393824	25.1361224398794	25.928880870140876	23.134057536041492
21	25.788380760689932	24.546152405531167	24.730963714830796	24.934503118948097
22	25.379724127617216	26.084051415137377	25.2179627811701	23.318261676075306
23	23.1051235718882	26.066101664684084	26.28144178407814	24.547332979349576
24	24.819355958457873	25.487599994786308	26.18513673243004	23.507907314325777
25	24.194778805137503	25.663831556301343	26.283174427203885	23.858215211357273
26	22.98897730492464	26.84139791400967	26.8506595299627	23.31896525110299
27	24.005134435915256	26.395144376852777	25.67190515426291	23.92781603296906
28	23.15295741544043	26.18422505953112	25.98280372190409	24.68001380312436
29	23.8123594064181	25.481817077931122	25.750878634852185	24.954944880798596
30	23.32651411934036	25.65828740311434	26.659010486771134	24.356187990774167
31	24.935321362439804	25.0254015015186	25.63586081417901	24.40341632186258
32	23.77879280159379	26.25749833980034	25.604051984830438	24.35965687377543
33	22.96917191719172	26.133825272771176	26.735554653026277	24.161448157010824
34	23.96784986358282	26.07362068409596	26.771588181339833	23.186941270981386
35	24.231044434946718	26.1537849387271	26.333662399779374	23.281508226546812
36	23.10898858597702	26.297189956318395	26.5287360737874	24.065085383917186
37	24.23320017370437	25.9927458530826	25.24819075099698	24.52586322221605
38	23.926585766112876	26.578425238338298	25.518800871574488	23.976188123974335
39	24.91506842548661	25.32195789918526	25.451070357371208	24.311903317956922
40	25.592333502782182	25.352223793225747	26.071180866411876	22.98426183758019
41	24.505489303695736	25.2829034070523	26.87443445044877	23.3371728388032
42	24.995506552201515	26.73731784385852	25.881298887683013	22.38587671625695
43	23.44914308921449	26.197623892245065	26.503315547054974	23.849917471485472
44	23.28813359329015	26.4469244922404	25.83060182015507	24.434340094314383
45	23.511575226969946	26.80934524814054	25.650930724259474	24.028148800630042
46	23.696344007535213	25.340041668696134	26.036966732958817	24.926647590809836
47	24.91107426405164	25.343680390100136	26.09123969164637	23.654005654201857
48	24.18876061020377	26.271320738407756	25.17100839211979	24.368910259268688
49	24.331840798408997	25.894371883358637	25.505539895410507	24.268247422821858
50	23.748934949968376	27.16299484522645	24.859948493425847	24.22812171137933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	14.5
2	18.0
3	61.5
4	105.0
5	121.5
6	138.0
7	138.0
8	138.0
9	200.5
10	263.0
11	416.0
12	569.0
13	1000.0
14	1431.0
15	2031.0
16	2631.0
17	2983.0
18	3335.0
19	3620.5
20	3906.0
21	4371.0
22	4836.0
23	5699.0
24	6562.0
25	8091.0
26	9620.0
27	13199.5
28	16779.0
29	21498.0
30	26217.0
31	31711.0
32	37205.0
33	45629.5
34	54054.0
35	63746.0
36	73438.0
37	77894.5
38	82351.0
39	81189.0
40	80027.0
41	80805.0
42	81583.0
43	79923.0
44	78263.0
45	83832.0
46	89401.0
47	94631.0
48	99861.0
49	101118.5
50	102376.0
51	97826.0
52	93276.0
53	91189.5
54	89103.0
55	89118.5
56	89134.0
57	86633.5
58	84133.0
59	76162.5
60	68192.0
61	60116.0
62	52040.0
63	45127.0
64	38214.0
65	32755.0
66	27296.0
67	23385.5
68	19475.0
69	17693.0
70	15911.0
71	12511.5
72	9112.0
73	7792.0
74	6472.0
75	4991.5
76	3511.0
77	3054.5
78	2598.0
79	2135.5
80	1673.0
81	1364.0
82	1055.0
83	871.5
84	688.0
85	481.0
86	274.0
87	222.0
88	170.0
89	127.0
90	84.0
91	65.5
92	47.0
93	44.5
94	42.0
95	43.0
96	44.0
97	32.0
98	20.0
99	20.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007203113391411008
2	2.744043196728003E-4
3	6.860107991820007E-4
4	0.0
5	0.0
6	6.860107991820007E-5
7	2.744043196728003E-4
8	0.0
9	0.0013720215983640013
10	8.232129590184009E-4
11	6.860107991820007E-5
12	6.860107991820007E-5
13	4.1160647950920043E-4
14	2.0580323975460021E-4
15	0.0045962723545194045
16	6.174097192638006E-4
17	0.0
18	6.860107991820007E-5
19	0.0
20	5.488086393456006E-4
21	0.0
22	0.0
23	6.174097192638006E-4
24	1.3720215983640014E-4
25	0.001989431317627802
26	0.0050764799139468055
27	0.006448501512310806
28	0.004253266954928404
29	0.0030184475164008033
30	0.003910261555337404
31	0.0076833209508384084
32	0.003224250756155403
33	0.0048706766741922055
34	0.002881245356564403
35	0.0030184475164008033
36	0.007546118791002008
37	0.0037044583155828037
38	0.007614719870920207
39	0.004047463715173804
40	0.0024696388770552025
41	0.0034300539959100035
42	0.0017150269979550018
43	0.0017150269979550018
44	0.0017150269979550018
45	0.0014406226782822016
46	9.604151188548011E-4
47	0.0017836280778732018
48	0.0017836280778732018
49	2.744043196728003E-4
50	0.0017150269979550018
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1457703.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.0366737390115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.90879559088481	43.86621881777623
2	13.763984796435686	15.701038203660337
3	4.4359545428029	7.590362759368048
4	1.8791746840387042	4.287274934085024
5	0.9580573141247861	2.732220122450454
6	0.5208194648053546	1.7823485934617758
7	0.32537098487941446	1.2990655096095607
8	0.23158629534589936	1.0567129576056336
9	0.15688145206521995	0.8053196577431689
>10	0.6787024963332133	7.116301851405367
>50	0.06700218174167023	2.6779011252838267
>100	0.06557864380678277	7.591771647795861
>500	0.0066423194097598665	2.5052012315769985
>1k	0.001449233325765789	0.9882625881777117
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1476	0.1012551939592633	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.860107991820007E-5
2	0.0	0.0	0.0	0.0	6.860107991820007E-5
3	0.0	0.0	0.0	0.0	6.860107991820007E-5
4	0.0	0.0	0.0	0.0	6.860107991820007E-5
5	0.0	0.0	0.0	0.0	1.3720215983640014E-4
6	0.0	0.0	0.0	0.0	1.3720215983640014E-4
7	0.0	0.0	0.0	0.0	1.3720215983640014E-4
8	0.0	0.0	0.0	0.0	1.3720215983640014E-4
9	0.0	0.0	0.0	0.0	1.3720215983640014E-4
10	0.0	0.0	0.0	0.0	1.3720215983640014E-4
11	0.0	0.0	0.0	0.0	1.3720215983640014E-4
12	0.0	0.0	0.0	0.0	0.001029016198773001
13	0.0	0.0	0.0	0.0	0.0013034205184458013
14	0.0	0.0	0.0	0.0	0.0013034205184458013
15	0.0	0.0	0.0	0.0	0.0017150269979550018
16	6.860107991820007E-5	0.0	0.0	0.0	0.0017150269979550018
17	6.860107991820007E-5	0.0	0.0	0.0	0.0017150269979550018
18	6.860107991820007E-5	0.0	0.0	6.860107991820007E-5	0.0017836280778732018
19	6.860107991820007E-5	0.0	0.0	6.860107991820007E-5	0.0018522291577914018
20	6.860107991820007E-5	0.0	0.0	1.3720215983640014E-4	0.002126633477464202
21	6.860107991820007E-5	0.0	0.0	2.0580323975460021E-4	0.0022638356373006025
22	6.860107991820007E-5	0.0	0.0	4.8020755942740053E-4	0.0024010377971370026
23	6.860107991820007E-5	0.0	0.0	0.001029016198773001	0.0024010377971370026
24	6.860107991820007E-5	0.0	0.0	0.0024696388770552025	0.0024010377971370026
25	6.860107991820007E-5	0.0	0.0	0.0031556496762372033	0.0024010377971370026
26	6.860107991820007E-5	0.0	0.0	0.0039102615553374045	0.0024010377971370026
27	6.860107991820007E-5	0.0	0.0	0.004390469114764805	0.0024010377971370026
28	6.860107991820007E-5	0.0	0.0	0.006105496112719807	0.0024010377971370026
29	6.860107991820007E-5	0.0	0.0	0.008163528510265809	0.0024010377971370026
30	6.860107991820007E-5	0.0	0.0	0.013445811663967215	0.0024010377971370026
31	6.860107991820007E-5	0.0	0.0	0.023735973651697224	0.0024010377971370026
32	6.860107991820007E-5	0.0	0.0	0.03505515183820024	0.0024696388770552025
33	6.860107991820007E-5	0.0	0.0	0.04465930302674825	0.0024696388770552025
34	6.860107991820007E-5	0.0	0.0	0.05721330065177886	0.0025382399569734027
35	6.860107991820007E-5	0.0	0.0	0.07189393175427368	0.0025382399569734027
36	6.860107991820007E-5	0.0	0.0	0.0937090751682613	0.0025382399569734027
37	6.860107991820007E-5	0.0	0.0	0.12725500324826114	0.0025382399569734027
38	6.860107991820007E-5	0.0	0.0	0.1747955516315738	0.0025382399569734027
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGGA	2375	0.0	20.377485	2
GCATTCG	425	0.0	20.187479	22
TTAGGAC	1430	0.0	19.999252	3
CTGTAGG	2285	0.0	19.640404	1
GTCCTAA	1210	0.0	19.453817	1
GATATAC	650	0.0	19.291586	1
TAGGACG	2270	0.0	19.091797	4
GTAGGAC	2490	0.0	18.906923	3
TAGGACC	1415	0.0	18.812017	4
AGGACCT	1575	0.0	18.577084	5
AGGACGT	2295	0.0	18.500397	5
GGACGTG	2290	0.0	18.444725	6
TAGGACA	835	0.0	18.442423	4
TTCGTAT	490	0.0	18.407475	25
GGCGAGA	1470	0.0	18.407475	19
GCGAGAA	1460	0.0	18.382874	20
CGCCTAA	60	0.0074144145	18.332647	39
TTTAGGA	1590	0.0	18.26284	2
TATGGCG	2635	0.0	18.116348	16
TGGCGAG	2675	0.0	18.092161	18
>>END_MODULE