FastQCFastQC Report
Thu 2 Feb 2017
SRR4063892_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063892_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39317
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG2910.7401378538545668No Hit
GTACATGATGCCTTGCGCGTCGCCC770.1958440369305898No Hit
GTACATGGGGCTGGATCGAAGGGGC720.18312689167535673No Hit
GTATCAACGCAGAGTACATGGGGCT680.17295317547117023No Hit
TTCCAAAGGGTGATGGCAACACGCG630.16023603021593713No Hit
CCCTTTGGAATGTCCACGCGTTTGA550.13988859780756416No Hit
ATATAGCGCTGTCCACCGGCAGTCG510.12971488160337769No Hit
CCATAAGAGTCTCTGTGGAATATCG500.12717145255233106No Hit
GTGCTAACCCTGCCCTGTGCCAGCC460.11699773634814457No Hit
CTGTACATGATGCCTTGCGCGTCGC420.10682402014395807No Hit
GTATCAACGCAGAGTACATGGGTGC400.10173716204186485No Hit
GCCTTCATCAGAAGGTCATGATCCA400.10173716204186485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG800.0043524339.5000011