##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063892_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39317
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33006078795432	32.0	32.0	32.0	32.0	32.0
2	31.0087493959356	32.0	32.0	32.0	32.0	32.0
3	31.010122847623165	32.0	32.0	32.0	32.0	32.0
4	31.041050944883892	32.0	32.0	32.0	32.0	32.0
5	30.98473942569372	32.0	32.0	32.0	32.0	32.0
6	34.643436681333775	36.0	36.0	36.0	32.0	36.0
7	34.6145941958949	36.0	36.0	36.0	32.0	36.0
8	34.5394867360175	36.0	36.0	36.0	32.0	36.0
9	34.63623877711931	36.0	36.0	36.0	32.0	36.0
10	34.39934379530483	36.0	36.0	36.0	32.0	36.0
11	34.64013022356741	36.0	36.0	36.0	32.0	36.0
12	34.4743495180202	36.0	36.0	36.0	32.0	36.0
13	34.52771065951115	36.0	36.0	36.0	32.0	36.0
14	34.45400208561182	36.0	36.0	36.0	32.0	36.0
15	34.45194190808047	36.0	36.0	36.0	32.0	36.0
16	34.43820739120482	36.0	36.0	36.0	32.0	36.0
17	34.37436732202355	36.0	36.0	36.0	32.0	36.0
18	34.365745097540504	36.0	36.0	36.0	32.0	36.0
19	34.36447338301498	36.0	36.0	36.0	32.0	36.0
20	34.343388356181805	36.0	36.0	36.0	32.0	36.0
21	34.32192181499097	36.0	36.0	36.0	32.0	36.0
22	34.2758094462955	36.0	36.0	36.0	32.0	36.0
23	34.23330874685251	36.0	36.0	36.0	32.0	36.0
24	34.265533992929264	36.0	36.0	36.0	32.0	36.0
25	33.88666480148536	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	3.0
9	2.0
10	6.0
11	1.0
12	2.0
13	1.0
14	3.0
15	4.0
16	7.0
17	18.0
18	17.0
19	11.0
20	24.0
21	33.0
22	76.0
23	118.0
24	185.0
25	243.0
26	407.0
27	483.0
28	702.0
29	948.0
30	1258.0
31	1681.0
32	2345.0
33	3509.0
34	7790.0
35	19439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63534590234842	19.09014579039768	12.08813576571763	25.18637254153627
2	16.709060385613267	20.282850892811723	37.76262908887419	25.24545963270082
3	18.75270239336674	23.704758755754508	28.705140270112167	28.83739858076659
4	14.075336368492	16.143144186992902	36.34305771040517	33.43846173410993
5	15.655602187460257	35.347831616431385	34.03535546229175	14.96121073381661
6	32.44900035610724	36.7222872259246	17.367858778043445	13.460853639924707
7	29.61662723549314	30.997990282123688	21.193619781729375	18.191762700653793
8	27.147364135965805	33.3375737838388	19.9292692855689	19.5857927946265
9	26.993537224568726	15.20533306193069	19.693654266958426	38.10747544654216
10	16.681511642702635	26.810026720956863	32.4697798702125	24.038681766128008
11	34.82963076061786	22.21798101636257	23.312212128152275	19.640176094867293
12	24.081237911025145	24.541891479181512	29.680342054362214	21.69652855543113
13	29.016823191061565	20.676490799419714	25.384948206968517	24.921737802550204
14	22.36982745457322	20.364432228839007	26.586756247773195	30.67898406881458
15	25.928847719869708	27.27758550488599	22.002239413680783	24.791327361563518
16	24.062810169750335	28.02534802636602	23.93301605884	23.978825745043647
17	23.870114006514658	26.677015472312704	25.0	24.45287052117264
18	24.05446678544159	26.62764062102316	25.874268261644183	23.443624331891066
19	24.29564022295182	26.02631645924003	26.44880506986333	23.22923824794482
20	25.47726925622359	25.319452222165655	26.083083032123405	23.12019548948735
21	25.65277141548328	25.925077619992877	24.884206240138443	23.5379447243854
22	25.350607040138463	25.149532948153425	25.928376899386596	23.571483112321516
23	24.800814560264733	25.307369224894998	25.811378388697975	24.080437826142294
24	24.421652711678927	25.363296261420608	25.816303158323368	24.3987478685771
25	25.027361347959985	25.26152358166408	25.528773957087225	24.182341113288707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	4.0
2	7.0
3	10.0
4	10.0
5	10.0
6	15.5
7	21.0
8	21.0
9	21.0
10	41.5
11	62.0
12	62.0
13	62.0
14	124.5
15	187.0
16	187.0
17	187.0
18	298.5
19	410.0
20	410.0
21	410.0
22	663.0
23	916.0
24	916.0
25	916.0
26	1203.5
27	1491.0
28	1491.0
29	1491.0
30	1919.0
31	2347.0
32	2347.0
33	2347.0
34	2713.0
35	3079.0
36	3079.0
37	3079.0
38	3326.0
39	3573.0
40	3573.0
41	3573.0
42	3774.5
43	3976.0
44	3976.0
45	3976.0
46	4174.0
47	4372.0
48	4372.0
49	4372.0
50	4415.5
51	4459.0
52	4459.0
53	4459.0
54	4494.5
55	4530.0
56	4530.0
57	4530.0
58	4036.0
59	3542.0
60	3542.0
61	3542.0
62	3195.5
63	2849.0
64	2849.0
65	2849.0
66	2294.5
67	1740.0
68	1740.0
69	1740.0
70	1273.0
71	806.0
72	806.0
73	806.0
74	612.5
75	419.0
76	419.0
77	419.0
78	270.0
79	121.0
80	121.0
81	121.0
82	90.0
83	59.0
84	59.0
85	59.0
86	41.0
87	23.0
88	23.0
89	23.0
90	18.0
91	13.0
92	13.0
93	13.0
94	10.0
95	7.0
96	7.0
97	7.0
98	154.0
99	301.0
100	301.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035608006714652696
2	0.007630287153139863
3	0.0
4	0.0
5	0.0050868581020932415
6	0.007630287153139863
7	0.020347432408372966
8	0.03306457766360608
9	0.038151435765699314
10	0.05595543912302566
11	0.050868581020932425
12	0.06358572627616553
13	0.06612915532721216
14	0.05849886817407229
15	0.05341201007197904
16	0.061042297225118905
17	0.05341201007197904
18	0.06867258437825877
19	0.06612915532721216
20	0.07884630058244525
21	0.05849886817407229
22	0.07121601342930539
23	0.08138972963349186
24	0.061042297225118905
25	0.07121601342930539
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	39317.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.94127222321133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.07966678787479	69.30844164102042
2	6.062609500512379	9.329297759239006
3	1.3421043932431986	3.0978965841747845
4	0.7239430101484249	2.22804384871684
5	0.4065981289874715	1.564208866393672
6	0.27767677101583416	1.281888241727497
7	0.21486892995272885	1.1572602182262126
8	0.14214406135334368	0.8749395935600377
9	0.08594757198109154	0.5951623979449093
>10	0.6413011140127599	8.840959381438054
>50	0.019834055072559587	0.9817636137039957
>100	0.0033056758454265978	0.7401378538545668
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	291	0.7401378538545668	No Hit
GTACATGATGCCTTGCGCGTCGCCC	77	0.1958440369305898	No Hit
GTACATGGGGCTGGATCGAAGGGGC	72	0.18312689167535673	No Hit
GTATCAACGCAGAGTACATGGGGCT	68	0.17295317547117023	No Hit
TTCCAAAGGGTGATGGCAACACGCG	63	0.16023603021593713	No Hit
CCCTTTGGAATGTCCACGCGTTTGA	55	0.13988859780756416	No Hit
ATATAGCGCTGTCCACCGGCAGTCG	51	0.12971488160337769	No Hit
CCATAAGAGTCTCTGTGGAATATCG	50	0.12717145255233106	No Hit
GTGCTAACCCTGCCCTGTGCCAGCC	46	0.11699773634814457	No Hit
CTGTACATGATGCCTTGCGCGTCGC	42	0.10682402014395807	No Hit
GTATCAACGCAGAGTACATGGGTGC	40	0.10173716204186485	No Hit
GCCTTCATCAGAAGGTCATGATCCA	40	0.10173716204186485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	80	0.004352433	9.500001	1
>>END_MODULE