FastQCFastQC Report
Thu 2 Feb 2017
SRR4063891_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063891_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768061
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT42520.24048944012678297No Hit
GTCCTACAGTGGACATTTCTAAATT38690.21882729159231495No Hit
CTGTAGGACGTGGAATATGGCAAGA37380.21141804496564318No Hit
GTATCAACGCAGAGTACTTTTTTTT36480.20632772285571593No Hit
CTTTAGGACGTGAAATATGGCGAGG36460.20621460458660643No Hit
GTCCTACAGTGTGCATTTCTCATTT25630.1449610618638158No Hit
TATCAACGCAGAGTACTTTTTTTTT24820.1403797719648813No Hit
CTGTAGGACCTGGAATATGGCGAGA19850.1122698820911722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG1400.014.246371
CGCCCAT500.00149681213.3033914
TTATACC1001.01154E-813.2962374
TTCGGAA2750.012.783627518
CGAACGA1207.366907E-1012.67025316
AAATGTC6050.012.5623397
CACCTTT5300.012.55036714
GTCCTAG2100.012.2111741
ATTTAGA6750.012.1006211
TAGGACC11850.012.0219144
TTTAGAA6500.011.9781732
TAGAAAT6350.011.8155534
TTTCGGA3150.011.76456817
CGCGCTA658.0053357E-411.69561916
GCCGTTA658.0053357E-411.69561915
ACGAACG1302.6047928E-911.69561915
TCCAACG1302.6102498E-911.69396318
TATACCT1552.5465852E-1111.6428555
CCACCTT5800.011.63029913
AATGTCC6550.011.6037118
Produced by FastQC (version 0.11.5)