##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4063891_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1768061 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.239266066046365 32.0 32.0 32.0 32.0 32.0 2 30.781100312715456 32.0 32.0 32.0 32.0 32.0 3 30.80579968677551 32.0 32.0 32.0 32.0 32.0 4 30.85405085005551 32.0 32.0 32.0 32.0 32.0 5 30.733381370891614 32.0 32.0 32.0 32.0 32.0 6 34.406932792477186 36.0 36.0 36.0 32.0 36.0 7 34.3448908154187 36.0 36.0 36.0 32.0 36.0 8 34.29632065861981 36.0 36.0 36.0 32.0 36.0 9 34.455638125607656 36.0 36.0 36.0 32.0 36.0 10 34.10004914988793 36.0 36.0 36.0 32.0 36.0 11 34.42443388548246 36.0 36.0 36.0 32.0 36.0 12 34.22219199450698 36.0 36.0 36.0 32.0 36.0 13 34.31640820084827 36.0 36.0 36.0 32.0 36.0 14 34.214288421044294 36.0 36.0 36.0 32.0 36.0 15 34.143512016836524 36.0 36.0 36.0 32.0 36.0 16 34.15007966354102 36.0 36.0 36.0 32.0 36.0 17 34.07596344243779 36.0 36.0 36.0 32.0 36.0 18 34.07151902564448 36.0 36.0 36.0 32.0 36.0 19 34.06873631622438 36.0 36.0 36.0 32.0 36.0 20 34.04124178973463 36.0 36.0 36.0 32.0 36.0 21 34.02119214212632 36.0 36.0 36.0 32.0 36.0 22 33.98370531333478 36.0 36.0 36.0 32.0 36.0 23 33.94620830389902 36.0 36.0 36.0 32.0 36.0 24 33.91025931797602 36.0 36.0 36.0 32.0 36.0 25 33.51506480828433 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 14.0 5 25.0 6 71.0 7 28.0 8 89.0 9 115.0 10 151.0 11 47.0 12 99.0 13 90.0 14 176.0 15 284.0 16 494.0 17 686.0 18 909.0 19 1225.0 20 1885.0 21 2884.0 22 4375.0 23 6783.0 24 10205.0 25 14696.0 26 20911.0 27 27082.0 28 37137.0 29 49740.0 30 65572.0 31 88535.0 32 124377.0 33 174260.0 34 368600.0 35 766510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.87220762731285 17.647122061788238 11.420430144639544 26.060240166259373 2 15.866507690689158 19.652688771718264 39.80029208442167 24.680511453170904 3 18.87212384850718 24.474061823576623 28.583944512285136 28.06986981563106 4 12.074715270204843 15.825863465445543 36.81074348752709 35.28867777682252 5 13.821720865223572 37.51100874307729 34.1831300522141 14.48414033948503 6 33.33108954307002 36.3906955485841 17.409757206809207 12.868457701536675 7 29.776975206078575 30.41974914731471 21.219912754271064 18.583362892335646 8 27.07183727092036 35.14328245220665 19.18783696086968 18.59704331600331 9 27.714212637278067 13.88206082335153 18.77348326407185 39.63024327529855 10 16.130981923009667 26.827491874890004 32.159816372231056 24.88170982986928 11 36.71487004667545 20.402739862463612 24.178765613186833 18.703624477674104 12 25.69678523485375 23.144339582064685 29.914282739058685 21.244592444022885 13 30.194876856434945 20.198227239748224 25.184016407823144 24.422879495993687 14 22.74557145355481 20.93068664754832 25.30074045398438 31.023001444912502 15 24.173252632264017 30.086388627148043 21.765463224703808 23.974895515884132 16 24.36147911365468 25.671120428140647 26.076520632793326 23.890879825411343 17 22.998105817297105 27.0508192184558 26.18844825859131 23.76262670565578 18 23.63372288827243 24.865046297737557 28.75169028203159 22.749540531958427 19 25.98306736715558 24.324330443307147 25.292936359427337 24.399665830109928 20 25.971854456453197 24.79342538578142 25.6105540693982 23.62416608836718 21 26.142290002365915 24.13545035596273 24.3720418982113 25.350217743460057 22 25.892868261107292 25.87017085879952 24.75918678773457 23.477774092358615 23 23.379419065058713 25.84348210015085 26.23360230041859 24.54349653437184 24 25.129718883458192 25.01883449698733 26.161356876218722 23.690089743335758 25 24.26884531047825 25.453748911829 26.314380384960657 23.963025392732092 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 146.0 1 146.0 2 375.5 3 605.0 4 605.0 5 605.0 6 1545.5 7 2486.0 8 2486.0 9 2486.0 10 2607.0 11 2728.0 12 2728.0 13 2728.0 14 3180.5 15 3633.0 16 3633.0 17 3633.0 18 6710.5 19 9788.0 20 9788.0 21 9788.0 22 18052.0 23 26316.0 24 26316.0 25 26316.0 26 43048.0 27 59780.0 28 59780.0 29 59780.0 30 80361.0 31 100942.0 32 100942.0 33 100942.0 34 117389.5 35 133837.0 36 133837.0 37 133837.0 38 147168.0 39 160499.0 40 160499.0 41 160499.0 42 179001.0 43 197503.0 44 197503.0 45 197503.0 46 214153.5 47 230804.0 48 230804.0 49 230804.0 50 230472.0 51 230140.0 52 230140.0 53 230140.0 54 209894.5 55 189649.0 56 189649.0 57 189649.0 58 172178.5 59 154708.0 60 154708.0 61 154708.0 62 135284.5 63 115861.0 64 115861.0 65 115861.0 66 94473.5 67 73086.0 68 73086.0 69 73086.0 70 54730.0 71 36374.0 72 36374.0 73 36374.0 74 27669.0 75 18964.0 76 18964.0 77 18964.0 78 15088.5 79 11213.0 80 11213.0 81 11213.0 82 7743.0 83 4273.0 84 4273.0 85 4273.0 86 3307.5 87 2342.0 88 2342.0 89 2342.0 90 1667.0 91 992.0 92 992.0 93 992.0 94 712.5 95 433.0 96 433.0 97 433.0 98 696.0 99 959.0 100 959.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03970451245743219 2 0.004468171629824989 3 0.001696774036642401 4 0.0039025802842775218 5 0.00837075191410251 6 0.012386450467489528 7 0.023189245167446144 8 0.03438795380928599 9 0.04779246869876096 10 0.057011607631184666 11 0.054579564845330567 12 0.06159289753011916 13 0.06215848887566663 14 0.06628730569816313 15 0.059160854744265044 16 0.0658913917562799 17 0.06063139224268846 18 0.07601547684157955 19 0.07324407924839697 20 0.07844751962743367 21 0.07369655232483495 22 0.07573268116880583 23 0.0806533258750688 24 0.07669418645623652 25 0.07550644463058684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1768061.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.96131706550424 #Duplication Level Percentage of deduplicated Percentage of total 1 72.67309579860459 33.4015119813143 2 15.031525324248351 13.817374028118698 3 5.189456451229011 7.155427600576891 4 2.3887951096145055 4.3916867775007296 5 1.2906515448722728 2.96600224374787 6 0.7511884060418498 2.0715365103612116 7 0.503627087673908 1.6203154981549888 8 0.3610260959740102 1.3274587892786116 9 0.2501501006654041 1.0347505281585396 >10 1.3205371959365033 11.038468019511498 >50 0.12047284420991708 3.8553533429343707 >100 0.10288426876890347 9.861049537839953 >500 0.01163760136650321 3.6481057047748298 >1k 0.004952170794256685 3.8109594377275617 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTAAAGTGTGTATTTCTCATTT 4252 0.24048944012678297 No Hit GTCCTACAGTGGACATTTCTAAATT 3869 0.21882729159231495 No Hit CTGTAGGACGTGGAATATGGCAAGA 3738 0.21141804496564318 No Hit GTATCAACGCAGAGTACTTTTTTTT 3648 0.20632772285571593 No Hit CTTTAGGACGTGAAATATGGCGAGG 3646 0.20621460458660643 No Hit GTCCTACAGTGTGCATTTCTCATTT 2563 0.1449610618638158 No Hit TATCAACGCAGAGTACTTTTTTTTT 2482 0.1403797719648813 No Hit CTGTAGGACCTGGAATATGGCGAGA 1985 0.1122698820911722 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.131182691094934E-4 2 0.0 0.0 0.0 0.0 1.131182691094934E-4 3 0.0 0.0 0.0 0.0 1.131182691094934E-4 4 0.0 0.0 0.0 0.0 1.131182691094934E-4 5 0.0 0.0 0.0 0.0 1.131182691094934E-4 6 0.0 0.0 0.0 0.0 2.827956727737335E-4 7 0.0 0.0 0.0 0.0 2.827956727737335E-4 8 0.0 0.0 0.0 0.0 2.827956727737335E-4 9 0.0 0.0 0.0 0.0 2.827956727737335E-4 10 0.0 0.0 0.0 0.0 2.827956727737335E-4 11 0.0 0.0 0.0 0.0 2.827956727737335E-4 12 0.0 0.0 0.0 0.0 5.65591345547467E-4 13 0.0 0.0 0.0 5.65591345547467E-5 5.65591345547467E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTAG 140 0.0 14.24637 1 CGCCCAT 50 0.001496812 13.30339 14 TTATACC 100 1.01154E-8 13.296237 4 TTCGGAA 275 0.0 12.7836275 18 CGAACGA 120 7.366907E-10 12.670253 16 AAATGTC 605 0.0 12.562339 7 CACCTTT 530 0.0 12.550367 14 GTCCTAG 210 0.0 12.211174 1 ATTTAGA 675 0.0 12.100621 1 TAGGACC 1185 0.0 12.021914 4 TTTAGAA 650 0.0 11.978173 2 TAGAAAT 635 0.0 11.815553 4 TTTCGGA 315 0.0 11.764568 17 CGCGCTA 65 8.0053357E-4 11.695619 16 GCCGTTA 65 8.0053357E-4 11.695619 15 ACGAACG 130 2.6047928E-9 11.695619 15 TCCAACG 130 2.6102498E-9 11.693963 18 TATACCT 155 2.5465852E-11 11.642855 5 CCACCTT 580 0.0 11.630299 13 AATGTCC 655 0.0 11.603711 8 >>END_MODULE