FastQCFastQC Report
Thu 2 Feb 2017
SRR4063891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768061
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19480.11017719411264656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC4650.022.2354741
GTATCAA33050.022.2318951
TGTAGGA29350.020.8371412
TTAGGAC17550.020.808693
ACCGTCG3500.020.7418448
GTATTAG4000.020.3490051
TAGGACG29150.020.3008944
GTAGGAC29750.020.187823
CTGTAGG28700.020.0059911
TACCGTC3650.019.8894397
AGGACGT29150.019.8480875
GGACGTG29250.019.780236
GTCCTAC24100.019.7168791
AAATTTA13200.019.499644
TAGGACA9050.019.4465644
TAGGACC17300.019.0742134
TCCTACA26400.018.9990732
CTTTAGG18950.018.922561
CGTCGTA3750.018.77241710
ATACCGT3750.018.7724176
Produced by FastQC (version 0.11.5)