##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768061
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.314627153701146	32.0	32.0	32.0	32.0	32.0
2	31.399343687802627	32.0	32.0	32.0	32.0	32.0
3	31.484626944432346	32.0	32.0	32.0	32.0	32.0
4	31.572425951367062	32.0	32.0	32.0	32.0	32.0
5	31.5195335455055	32.0	32.0	32.0	32.0	32.0
6	35.11097354672718	36.0	36.0	36.0	36.0	36.0
7	35.130621058888806	36.0	36.0	36.0	36.0	36.0
8	35.07399179100721	36.0	36.0	36.0	36.0	36.0
9	35.18458752271556	36.0	36.0	36.0	36.0	36.0
10	35.04415854430362	36.0	36.0	36.0	36.0	36.0
11	35.181455843435266	36.0	36.0	36.0	36.0	36.0
12	35.09660639536758	36.0	36.0	36.0	36.0	36.0
13	35.12948252351022	36.0	36.0	36.0	36.0	36.0
14	35.07837851748328	36.0	36.0	36.0	36.0	36.0
15	35.05237658655442	36.0	36.0	36.0	36.0	36.0
16	35.06453057897889	36.0	36.0	36.0	36.0	36.0
17	35.03986174685149	36.0	36.0	36.0	36.0	36.0
18	35.03399713018951	36.0	36.0	36.0	36.0	36.0
19	35.01996084976706	36.0	36.0	36.0	36.0	36.0
20	35.013961056773496	36.0	36.0	36.0	36.0	36.0
21	34.99769238731017	36.0	36.0	36.0	36.0	36.0
22	34.97824000416275	36.0	36.0	36.0	36.0	36.0
23	34.935945083342716	36.0	36.0	36.0	36.0	36.0
24	34.9078798751853	36.0	36.0	36.0	36.0	36.0
25	34.87604613189251	36.0	36.0	36.0	32.0	36.0
26	34.818763606006804	36.0	36.0	36.0	32.0	36.0
27	34.79740687679893	36.0	36.0	36.0	32.0	36.0
28	34.76025205012723	36.0	36.0	36.0	32.0	36.0
29	34.731819207595215	36.0	36.0	36.0	32.0	36.0
30	34.697578873127114	36.0	36.0	36.0	32.0	36.0
31	34.696759896858765	36.0	36.0	36.0	32.0	36.0
32	34.650930595720396	36.0	36.0	36.0	32.0	36.0
33	34.645284297317794	36.0	36.0	36.0	32.0	36.0
34	34.63160094589497	36.0	36.0	36.0	32.0	36.0
35	34.60136499815334	36.0	36.0	36.0	32.0	36.0
36	34.55585355935118	36.0	36.0	36.0	32.0	36.0
37	34.51678194360941	36.0	36.0	36.0	32.0	36.0
38	34.472283478907116	36.0	36.0	36.0	32.0	36.0
39	34.41151691033284	36.0	36.0	36.0	32.0	36.0
40	34.430581863408555	36.0	36.0	36.0	32.0	36.0
41	34.4156315873717	36.0	36.0	36.0	32.0	36.0
42	34.36216284392903	36.0	36.0	36.0	32.0	36.0
43	34.36473741573396	36.0	36.0	36.0	32.0	36.0
44	34.26854390204863	36.0	36.0	36.0	32.0	36.0
45	34.24149449594782	36.0	36.0	36.0	32.0	36.0
46	34.17379038392906	36.0	36.0	36.0	32.0	36.0
47	34.15421470186832	36.0	36.0	36.0	32.0	36.0
48	34.11337674435441	36.0	36.0	36.0	32.0	36.0
49	34.084237478231806	36.0	36.0	36.0	32.0	36.0
50	33.51459593305887	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	1.0
19	6.0
20	19.0
21	46.0
22	181.0
23	543.0
24	1471.0
25	3359.0
26	6921.0
27	12406.0
28	20490.0
29	31875.0
30	45458.0
31	64135.0
32	93632.0
33	150662.0
34	312856.0
35	1023998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1031986116799	18.465324804908896	11.923805300323775	26.50767128308743
2	15.131486294261206	20.371029181784504	39.244367398073024	25.253117125881264
3	18.207105313912393	24.991841326382577	28.949406042904723	27.851647316800303
4	11.803382349364643	16.457633531874748	37.04436668191878	34.69461743684183
5	13.524646491269248	37.92244724588122	33.927223099203026	14.625683163646503
6	33.453125919088535	36.61959802178212	17.118462311148516	12.808813747980832
7	29.738057680136603	30.59685157921588	21.231676961371807	18.433413779275714
8	27.089280290668704	35.3596963000711	19.04725006659838	18.50377334266182
9	27.661252007873127	13.734983371416936	18.6706183118029	39.933146308907034
10	16.045668624200456	27.087437451959538	32.033306919918715	24.83358700392129
11	36.73617595772997	20.328257905128837	24.217546792785996	18.7180193443552
12	25.60013483697678	23.266109031306044	29.81147143678866	21.322284694928513
13	30.285172930629788	20.065835242216	25.284522496535732	24.364469330618476
14	22.848923988310332	20.894631790717156	25.24686704105128	31.009577179921237
15	24.21747737556561	30.054468325791856	21.816742081447966	23.91131221719457
16	24.36848505415571	25.704137326433074	25.975170385452902	23.952207233958315
17	23.018606258494476	27.189107163157832	26.02291436777351	23.769372210574183
18	23.708498250623002	24.792229666673833	28.738480298723236	22.760791783979936
19	26.14892811956149	24.306344634036947	25.353310773779864	24.1914164726217
20	26.119744032650882	24.774128157878962	25.570830491199846	23.53529731827031
21	26.17568059020588	24.11166809289951	24.42670247236945	25.28594884452516
22	25.873118487561786	25.79048549850429	24.850358500479906	23.48603751345402
23	23.311667230377488	25.736943495814025	26.375444982766282	24.5759442910422
24	25.29375533425751	24.957283520346955	26.0727478192113	23.676213326184225
25	24.391484085969083	25.41081010457396	26.298860144420544	23.89884566503642
26	23.405727136618346	26.316128846150583	26.6605918995186	23.61755211771247
27	24.226281649543314	25.988882076250768	25.51398337967687	24.27085289452905
28	23.39796978604529	25.914385310784954	25.777393663203036	24.91025123996672
29	24.31432236520606	25.113346364771676	25.347109102097697	25.22522216792456
30	23.51322872225198	25.375719241248206	26.448621019463136	24.662431017036678
31	25.416425569992136	24.667371432546947	25.33384465625129	24.582358341209627
32	24.138463487639168	26.014194538021407	25.284162253026576	24.563179721312846
33	23.233227639397782	25.924407154306735	26.610380780973735	24.23198442532175
34	24.414046260919733	25.604647019397458	26.510974171599223	23.470332548083586
35	24.567976439140573	25.739062463765904	26.339396057843334	23.35356503925019
36	23.377731120005247	25.92332614612581	26.380520782191414	24.31842195167753
37	24.40000271491984	25.75191967457161	24.916063728218425	24.932013882290125
38	24.185732652540615	26.452552944417203	25.4032520352795	23.95846236776269
39	25.385270631195638	24.92712013384782	25.1650755835197	24.522533651436838
40	25.998191200001358	25.025126935565883	25.821779467801043	23.15490239663172
41	24.959248152450936	24.960096560102897	26.444527148479256	23.63612813896691
42	25.155299703116402	26.526880437186414	25.756250024745015	22.561569834952174
43	23.716598530799146	26.03470743808384	26.235325525045873	24.013368506071142
44	23.33312971849117	26.261563626244595	25.612541316845057	24.79276533841918
45	23.70329647332839	26.406118410149993	25.580967862170617	24.309617254351
46	24.074276221982537	25.16044521390527	25.807372985703452	24.95790557840874
47	25.19593874980416	25.07795412626617	25.696722957285157	24.029384166644512
48	24.33865396547443	26.188120855893843	24.922356779295292	24.550868399336434
49	24.651778366623212	25.641905936184113	25.12812101433496	24.578194682857717
50	23.929702828617273	27.2734676932666	24.578218668500714	24.21861080961541
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	21.0
2	23.0
3	90.0
4	157.0
5	161.5
6	166.0
7	179.0
8	192.0
9	295.5
10	399.0
11	559.0
12	719.0
13	1312.0
14	1905.0
15	2681.0
16	3457.0
17	3791.5
18	4126.0
19	4320.0
20	4514.0
21	4912.0
22	5310.0
23	6078.0
24	6846.0
25	8175.0
26	9504.0
27	13031.0
28	16558.0
29	21775.5
30	26993.0
31	33894.5
32	40796.0
33	51293.5
34	61791.0
35	73877.0
36	85963.0
37	91234.0
38	96505.0
39	94031.5
40	91558.0
41	92562.5
42	93567.0
43	92732.5
44	91898.0
45	98155.0
46	104412.0
47	111144.5
48	117877.0
49	122525.5
50	127174.0
51	124795.0
52	122416.0
53	119766.5
54	117117.0
55	116991.5
56	116866.0
57	113088.5
58	109311.0
59	99460.0
60	89609.0
61	78278.0
62	66947.0
63	57057.0
64	47167.0
65	40383.0
66	33599.0
67	28737.0
68	23875.0
69	21481.5
70	19088.0
71	14937.0
72	10786.0
73	8998.5
74	7211.0
75	5602.0
76	3993.0
77	3491.5
78	2990.0
79	2462.5
80	1935.0
81	1560.0
82	1185.0
83	1003.0
84	821.0
85	594.0
86	367.0
87	246.0
88	125.0
89	101.5
90	78.0
91	69.0
92	60.0
93	42.0
94	24.0
95	26.5
96	29.0
97	22.0
98	15.0
99	16.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009558493739752191
2	3.3935480732848015E-4
3	2.2623653821898681E-4
4	0.0
5	0.0
6	2.8279567277373344E-4
7	0.0
8	0.0
9	0.0011877418256496805
10	0.0011311826910949338
11	0.0
12	0.0
13	6.221504801022137E-4
14	2.2623653821898681E-4
15	0.0034501072078395485
16	6.221504801022137E-4
17	0.0
18	1.6967740366424008E-4
19	0.0
20	0.001300860094759174
21	0.0
22	1.1311826910949341E-4
23	8.483870183212005E-4
24	4.5247307643797363E-4
25	0.001696774036642401
26	0.005203440379036696
27	0.0059387091282484036
28	0.003959139418832269
29	0.001640214902087654
30	0.0029410749968468283
31	0.005542795186365176
32	0.0032238706696205615
33	0.0035632254769490416
34	0.002601720189518348
35	0.0024886019204088546
36	0.0059387091282484036
37	0.002997634131401575
38	0.006900214415679097
39	0.004694408168043976
40	0.0021492471130803745
41	0.002432042785854108
42	0.0015836557675329075
43	0.0014139783638686674
44	9.615052874306938E-4
45	0.0011311826910949338
46	6.787096146569603E-4
47	0.0019230105748613876
48	0.0021492471130803745
49	3.3935480732848015E-4
50	0.0013574192293139206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1768061.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.134282275126985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21745777337532	37.950564355260916
2	14.483398561514694	14.811963806953212
3	4.926855916497901	7.557937234892494
4	2.237186089286375	4.575876199662279
5	1.1534734998830705	2.9491019769949793
6	0.7005134583476083	2.1492151750003208
7	0.44983988989959384	1.6101567936116514
8	0.29788930047430395	1.2185884461754544
9	0.2294169883281984	1.0557965735895247
>10	1.1238375868713064	10.268504519231895
>50	0.09426723819539254	3.357283475328301
>100	0.07548017932516367	8.045562006220942
>500	0.007815551183764058	2.7486847135193195
>1k	0.002567966817522476	1.7007647235588434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1948	0.11017719411264656	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.131182691094934E-4
2	0.0	0.0	0.0	0.0	1.131182691094934E-4
3	0.0	0.0	0.0	0.0	1.131182691094934E-4
4	0.0	0.0	0.0	0.0	1.131182691094934E-4
5	5.65591345547467E-5	0.0	0.0	0.0	1.131182691094934E-4
6	5.65591345547467E-5	0.0	0.0	0.0	1.696774036642401E-4
7	5.65591345547467E-5	0.0	0.0	0.0	1.696774036642401E-4
8	5.65591345547467E-5	0.0	0.0	0.0	1.696774036642401E-4
9	5.65591345547467E-5	0.0	0.0	0.0	1.696774036642401E-4
10	5.65591345547467E-5	0.0	0.0	0.0	1.696774036642401E-4
11	5.65591345547467E-5	0.0	0.0	0.0	2.262365382189868E-4
12	5.65591345547467E-5	0.0	0.0	0.0	6.787096146569604E-4
13	5.65591345547467E-5	0.0	0.0	0.0	8.483870183212005E-4
14	5.65591345547467E-5	0.0	0.0	0.0	8.483870183212005E-4
15	5.65591345547467E-5	0.0	0.0	0.0	0.0010746235565401872
16	5.65591345547467E-5	0.0	0.0	1.131182691094934E-4	0.001131182691094934
17	5.65591345547467E-5	0.0	0.0	2.262365382189868E-4	0.0011877418256496805
18	5.65591345547467E-5	0.0	0.0	2.827956727737335E-4	0.001300860094759174
19	5.65591345547467E-5	0.0	0.0	3.393548073284802E-4	0.001300860094759174
20	5.65591345547467E-5	0.0	0.0	5.090322109927202E-4	0.001527096632978161
21	5.65591345547467E-5	0.0	0.0	5.65591345547467E-4	0.001527096632978161
22	5.65591345547467E-5	0.0	0.0	7.918278837664537E-4	0.0016402149020876542
23	5.65591345547467E-5	0.0	0.0	0.0019795697094161344	0.0016402149020876542
24	5.65591345547467E-5	0.0	0.0	0.0028845158622920817	0.0016402149020876542
25	1.131182691094934E-4	0.0	0.0	0.0036197846115037886	0.0016402149020876542
26	1.131182691094934E-4	0.0	0.0	0.004128816822496509	0.0016402149020876542
27	1.131182691094934E-4	0.0	0.0	0.004864085571708216	0.0017533331711971475
28	1.131182691094934E-4	0.0	0.0	0.006221504801022137	0.0017533331711971475
29	1.131182691094934E-4	0.0	0.0	0.009445375470642699	0.0017533331711971475
30	1.131182691094934E-4	0.0	0.0	0.015384084598891101	0.0017533331711971475
31	1.131182691094934E-4	0.0	0.0	0.02980666391035151	0.0018098923057518943
32	1.131182691094934E-4	0.0	0.0	0.04519074850924261	0.0018098923057518943
33	1.131182691094934E-4	0.0	0.0	0.0599526826280315	0.001866451440306641
34	1.131182691094934E-4	0.0	0.0	0.07725977780178399	0.0019795697094161344
35	1.131182691094934E-4	0.0	0.0	0.09648988355039786	0.0019795697094161344
36	1.131182691094934E-4	0.0	0.0	0.12397762294400476	0.0019795697094161344
37	1.131182691094934E-4	0.0	0.0	0.1675281565511597	0.0019795697094161344
38	1.131182691094934E-4	0.0	0.0	0.22646277475720578	0.002036128843970881
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	465	0.0	22.235474	1
GTATCAA	3305	0.0	22.231895	1
TGTAGGA	2935	0.0	20.837141	2
TTAGGAC	1755	0.0	20.80869	3
ACCGTCG	350	0.0	20.741844	8
GTATTAG	400	0.0	20.349005	1
TAGGACG	2915	0.0	20.300894	4
GTAGGAC	2975	0.0	20.18782	3
CTGTAGG	2870	0.0	20.005991	1
TACCGTC	365	0.0	19.889439	7
AGGACGT	2915	0.0	19.848087	5
GGACGTG	2925	0.0	19.78023	6
GTCCTAC	2410	0.0	19.716879	1
AAATTTA	1320	0.0	19.4996	44
TAGGACA	905	0.0	19.446564	4
TAGGACC	1730	0.0	19.074213	4
TCCTACA	2640	0.0	18.999073	2
CTTTAGG	1895	0.0	18.92256	1
CGTCGTA	375	0.0	18.772417	10
ATACCGT	375	0.0	18.772417	6
>>END_MODULE