FastQCFastQC Report
Thu 2 Feb 2017
SRR4063890_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063890_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692499
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9110.1315525365379589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGT250.00603128918.9986271
GGACCGA508.7189226E-515.1967066
CGAACCT400.00527013214.2520616
TTTAACC400.00528278114.2469113
GTATCAA6300.014.0227971
CTAGACC551.961283E-413.8151874
TTAGAGC500.001500531413.2971173
GTCCTAA751.4768757E-512.6657511
GTCTATA853.937572E-612.2932291
TCAAGAC701.0914213E-412.2116383
TCCTAAA701.09363194E-412.2089922
GTTCTAT1103.803507E-812.0900351
GTCTTTA550.003067148212.0900351
CTAACAC802.8673356E-511.8724263
GGTATCA2100.011.7610551
GGATAGT658.028146E-411.6897736
TATGATG658.042757E-411.6872412
GTCTTAA907.449722E-611.6102721
GTAGGAC907.4617183E-611.6085953
TTATACA907.4617183E-611.6085954
Produced by FastQC (version 0.11.5)