##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063890_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692499
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31784017016631	32.0	32.0	32.0	32.0	32.0
2	30.95423675702059	32.0	32.0	32.0	32.0	32.0
3	30.953137838466194	32.0	32.0	32.0	32.0	32.0
4	30.99253139715725	32.0	32.0	32.0	32.0	32.0
5	30.91465836051749	32.0	32.0	32.0	32.0	32.0
6	34.57716762045866	36.0	36.0	36.0	32.0	36.0
7	34.523732164234175	36.0	36.0	36.0	32.0	36.0
8	34.49165558361817	36.0	36.0	36.0	32.0	36.0
9	34.624307038710526	36.0	36.0	36.0	32.0	36.0
10	34.36308355679936	36.0	36.0	36.0	32.0	36.0
11	34.58957197050104	36.0	36.0	36.0	32.0	36.0
12	34.43958475030289	36.0	36.0	36.0	32.0	36.0
13	34.491427424443934	36.0	36.0	36.0	32.0	36.0
14	34.41338110235538	36.0	36.0	36.0	32.0	36.0
15	34.35552109100519	36.0	36.0	36.0	32.0	36.0
16	34.35155718636417	36.0	36.0	36.0	32.0	36.0
17	34.31091308435102	36.0	36.0	36.0	32.0	36.0
18	34.28628344589667	36.0	36.0	36.0	32.0	36.0
19	34.301096463677204	36.0	36.0	36.0	32.0	36.0
20	34.28496358839507	36.0	36.0	36.0	32.0	36.0
21	34.27446826638017	36.0	36.0	36.0	32.0	36.0
22	34.24126677439246	36.0	36.0	36.0	32.0	36.0
23	34.189971393460496	36.0	36.0	36.0	32.0	36.0
24	34.183184380049646	36.0	36.0	36.0	32.0	36.0
25	33.79388562293953	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	5.0
5	9.0
6	35.0
7	14.0
8	27.0
9	51.0
10	48.0
11	18.0
12	30.0
13	41.0
14	44.0
15	92.0
16	175.0
17	249.0
18	301.0
19	434.0
20	570.0
21	857.0
22	1273.0
23	2015.0
24	2974.0
25	4571.0
26	6694.0
27	9000.0
28	12420.0
29	17169.0
30	22922.0
31	31508.0
32	44917.0
33	64519.0
34	142014.0
35	327501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1862144881644	17.399607647125105	12.154704911922847	26.259472952787643
2	16.28358355525004	19.742162633655475	37.936492348928155	26.03776146216633
3	18.855633599426994	23.31910444657525	28.69565970818267	29.12960224581509
4	13.215909287830915	16.168069362692712	35.6932225809992	34.92279876847718
5	14.95922325610279	36.207900015741686	33.74873091681602	15.084145811339503
6	33.88888327241059	35.08846675438001	17.341679099142556	13.680970874066842
7	30.356095686813745	30.47308988910105	20.589390792411844	18.581423631673363
8	27.792062960095226	33.31912840484913	19.475968150142723	19.412840484912923
9	27.498078512277296	14.842842530469305	18.376357321590586	39.28272163566281
10	15.979141621971518	27.276129323099685	31.899331884594044	24.845397170334753
11	36.5051248190348	21.29826822438819	22.795403122570875	19.401203834006143
12	24.536600872756697	23.545212842817097	29.047336936103807	22.870849348322402
13	29.75685102718448	20.05545553587271	24.86006074389599	25.32763269304682
14	23.63356828473914	19.430163367810213	25.588751231833168	31.347517115617478
15	25.223381997665033	26.938626301857564	22.30265515367988	25.535336546797517
16	25.47710599288766	25.90164976728123	23.73919313562714	24.882051104203967
17	24.065460734973897	25.89892194388213	25.227900283060173	24.8077170380838
18	24.507695642748754	25.915600838210857	25.655466435436086	23.921237083604307
19	25.56222560702446	25.149642620761814	25.30716116893214	23.980970603281587
20	25.875456859064126	24.833767611384012	24.97582928320276	24.314946246349102
21	26.160431455062543	24.764878956738883	24.565596892413698	24.509092695784872
22	26.01440845141664	24.54711650324082	24.93139004704063	24.507084998301913
23	25.146006974804852	24.842214189811234	24.93861275669551	25.073166078688402
24	24.9782865995442	24.713390339740656	25.13349586469914	25.174827196016007
25	24.918385308054816	24.68643917158378	25.22938100007081	25.16579452029059
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	19.0
2	65.5
3	112.0
4	112.0
5	112.0
6	235.5
7	359.0
8	359.0
9	359.0
10	512.0
11	665.0
12	665.0
13	665.0
14	1183.5
15	1702.0
16	1702.0
17	1702.0
18	3090.0
19	4478.0
20	4478.0
21	4478.0
22	6939.5
23	9401.0
24	9401.0
25	9401.0
26	13721.5
27	18042.0
28	18042.0
29	18042.0
30	23929.0
31	29816.0
32	29816.0
33	29816.0
34	36412.5
35	43009.0
36	43009.0
37	43009.0
38	51084.5
39	59160.0
40	59160.0
41	59160.0
42	67390.5
43	75621.0
44	75621.0
45	75621.0
46	83580.0
47	91539.0
48	91539.0
49	91539.0
50	94600.0
51	97661.0
52	97661.0
53	97661.0
54	91912.0
55	86163.0
56	86163.0
57	86163.0
58	77528.5
59	68894.0
60	68894.0
61	68894.0
62	58510.5
63	48127.0
64	48127.0
65	48127.0
66	38901.0
67	29675.0
68	29675.0
69	29675.0
70	22221.5
71	14768.0
72	14768.0
73	14768.0
74	11029.0
75	7290.0
76	7290.0
77	7290.0
78	5417.5
79	3545.0
80	3545.0
81	3545.0
82	2401.5
83	1258.0
84	1258.0
85	1258.0
86	884.5
87	511.0
88	511.0
89	511.0
90	372.0
91	233.0
92	233.0
93	233.0
94	166.0
95	99.0
96	99.0
97	99.0
98	225.5
99	352.0
100	352.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038267203273939746
2	0.00678701341084969
3	0.0015884499472201405
4	0.0033213044350966572
5	0.010108317845946348
6	0.013285217740386629
7	0.022671512883051095
8	0.036245539704750473
9	0.04722028479463508
10	0.05819502988451969
11	0.0544405118274539
12	0.06339359334814924
13	0.05963907529108345
14	0.06368240242946199
15	0.059350266209770706
16	0.0667148977832459
17	0.06064990707567809
18	0.07927809282035064
19	0.0746571475193466
20	0.07884487919838151
21	0.07436833843803384
22	0.07855607011706876
23	0.08375463358069832
24	0.07653440654787949
25	0.07624559746656674
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	692499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.174569731096234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.896099787557354	27.509551062048043
2	17.280263494226645	15.612569363833165
3	8.385855825753147	11.364822862662153
4	4.4277211248465855	8.000815868169198
5	2.6903905243576864	6.076861717323842
6	1.7076054053256777	4.6284203673649005
7	1.1287530792761125	3.5693654282263836
8	0.777720727577244	2.8106559391405685
9	0.5764677491834421	2.343751427989395
>10	2.049736379340927	14.437435777614862
>50	0.053673903162877026	1.6814602850557123
>100	0.02506919940749288	1.7489715531313188
>500	6.427999847980852E-4	0.21531834744047046
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	911	0.1315525365379589	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTAGT	25	0.006031289	18.998627	1
GGACCGA	50	8.7189226E-5	15.196706	6
CGAACCT	40	0.005270132	14.25206	16
TTTAACC	40	0.005282781	14.246911	3
GTATCAA	630	0.0	14.022797	1
CTAGACC	55	1.961283E-4	13.815187	4
TTAGAGC	50	0.0015005314	13.297117	3
GTCCTAA	75	1.4768757E-5	12.665751	1
GTCTATA	85	3.937572E-6	12.293229	1
TCAAGAC	70	1.0914213E-4	12.211638	3
TCCTAAA	70	1.09363194E-4	12.208992	2
GTTCTAT	110	3.803507E-8	12.090035	1
GTCTTTA	55	0.0030671482	12.090035	1
CTAACAC	80	2.8673356E-5	11.872426	3
GGTATCA	210	0.0	11.761055	1
GGATAGT	65	8.028146E-4	11.689773	6
TATGATG	65	8.042757E-4	11.687241	2
GTCTTAA	90	7.449722E-6	11.610272	1
GTAGGAC	90	7.4617183E-6	11.608595	3
TTATACA	90	7.4617183E-6	11.608595	4
>>END_MODULE