FastQCFastQC Report
Thu 2 Feb 2017
SRR4063890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692499
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2900.030.3551881
GATTTCG454.0676096E-529.33275641
GTATCAA7150.027.7017631
GCGATTC407.029889E-427.49945813
ATATGCT852.3500797E-723.293664
TACGACC500.002579630421.9995674
TAAGGTT500.002579630421.9995674
TAGGACG803.6124693E-621.9995674
AATAGAG956.8588815E-720.8416965
TCTATAC755.4691372E-520.5329283
ATTTCGT654.929893E-420.30729142
CTAATAG901.0011172E-519.5551723
TTCGTAT808.9778885E-519.2510125
GTAGGAC1605.2750693E-1119.249623
CGCTAGA808.982868E-519.2496237
GTACACT1658.185452E-1118.6662984
TGCGATT600.007409825518.33297212
TATCAAC11050.018.3163812
TCAACGC11200.018.0710744
ATCAACG11300.017.9111523
Produced by FastQC (version 0.11.5)