##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063890_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692499
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37497671476782	32.0	32.0	32.0	32.0	32.0
2	31.43856236615504	32.0	32.0	32.0	32.0	32.0
3	31.483147268082696	32.0	32.0	32.0	32.0	32.0
4	31.560626080326468	32.0	32.0	32.0	32.0	32.0
5	31.525197870321833	32.0	32.0	32.0	32.0	32.0
6	35.10649834873408	36.0	36.0	36.0	36.0	36.0
7	35.12459656981454	36.0	36.0	36.0	36.0	36.0
8	35.072517072226816	36.0	36.0	36.0	36.0	36.0
9	35.18096777035057	36.0	36.0	36.0	36.0	36.0
10	35.042535801495745	36.0	36.0	36.0	36.0	36.0
11	35.17675837799044	36.0	36.0	36.0	36.0	36.0
12	35.09090843452481	36.0	36.0	36.0	36.0	36.0
13	35.122839166554755	36.0	36.0	36.0	36.0	36.0
14	35.08493586272327	36.0	36.0	36.0	36.0	36.0
15	35.04535602217476	36.0	36.0	36.0	36.0	36.0
16	35.06198998121297	36.0	36.0	36.0	36.0	36.0
17	35.035062866516775	36.0	36.0	36.0	36.0	36.0
18	35.028983435355144	36.0	36.0	36.0	36.0	36.0
19	35.04175457293079	36.0	36.0	36.0	36.0	36.0
20	35.01865706665281	36.0	36.0	36.0	36.0	36.0
21	35.02092999412274	36.0	36.0	36.0	36.0	36.0
22	35.00880867698004	36.0	36.0	36.0	36.0	36.0
23	34.94302374443862	36.0	36.0	36.0	36.0	36.0
24	34.935924817219956	36.0	36.0	36.0	36.0	36.0
25	34.90351899425126	36.0	36.0	36.0	32.0	36.0
26	34.842945621582125	36.0	36.0	36.0	32.0	36.0
27	34.84829580981344	36.0	36.0	36.0	32.0	36.0
28	34.82579180619756	36.0	36.0	36.0	32.0	36.0
29	34.80530802210545	36.0	36.0	36.0	32.0	36.0
30	34.79745241509374	36.0	36.0	36.0	32.0	36.0
31	34.78491810096477	36.0	36.0	36.0	32.0	36.0
32	34.752702891989735	36.0	36.0	36.0	32.0	36.0
33	34.734903588308434	36.0	36.0	36.0	32.0	36.0
34	34.73188697745412	36.0	36.0	36.0	32.0	36.0
35	34.689473919817935	36.0	36.0	36.0	32.0	36.0
36	34.680442859845286	36.0	36.0	36.0	32.0	36.0
37	34.668281109431206	36.0	36.0	36.0	32.0	36.0
38	34.64670418296633	36.0	36.0	36.0	32.0	36.0
39	34.627215346159346	36.0	36.0	36.0	32.0	36.0
40	34.62475613683197	36.0	36.0	36.0	32.0	36.0
41	34.609189327349206	36.0	36.0	36.0	32.0	36.0
42	34.57394739920202	36.0	36.0	36.0	32.0	36.0
43	34.55840225040036	36.0	36.0	36.0	32.0	36.0
44	34.51585634058677	36.0	36.0	36.0	32.0	36.0
45	34.48252344046706	36.0	36.0	36.0	32.0	36.0
46	34.47064616699807	36.0	36.0	36.0	32.0	36.0
47	34.46079055709828	36.0	36.0	36.0	32.0	36.0
48	34.42348942020133	36.0	36.0	36.0	32.0	36.0
49	34.397993354503036	36.0	36.0	36.0	32.0	36.0
50	33.820272664653665	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	2.0
21	20.0
22	52.0
23	178.0
24	536.0
25	1157.0
26	2392.0
27	4440.0
28	7301.0
29	11329.0
30	16472.0
31	23616.0
32	33620.0
33	54475.0
34	114857.0
35	422050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33589292187358	18.108571420318906	12.84810428217189	26.707431375635625
2	15.671236595629157	20.513705454744997	37.27072135948407	26.544336590141775
3	18.00064116264159	23.909012667294352	29.10993917619265	28.980406993871412
4	12.9324374475631	16.920602051410903	35.65376989714065	34.493190603885346
5	14.617205223401045	36.48568445586203	33.726113683918676	15.170996636818249
6	33.85097697174139	35.45242795275648	17.062889803132723	13.633705272369411
7	30.271812666877494	30.590080274484148	20.65519228186611	18.48291477677224
8	27.763794604757553	33.66084283154199	19.269775118808834	19.305587444891618
9	27.492880784182343	14.680313996407179	18.24425408524575	39.58255113416473
10	15.904177816029378	27.39847882499556	32.04344894013063	24.653894418844434
11	36.51470976853396	21.296204037839768	22.845087140920057	19.343999052706213
12	24.61364509355984	23.511403643043014	29.085571366271097	22.789379897126057
13	29.934512162542692	19.970830114296852	24.889565989646133	25.205091733514323
14	23.723389814844232	19.410741980482253	25.44989299608085	31.415975208592663
15	25.21185216848818	27.19274937036438	22.14454469835255	25.45085376279489
16	25.396502202910355	25.848059113954942	23.7850779719073	24.970360711227404
17	24.020540101862963	25.980687336732615	25.22848408445355	24.77028847695087
18	24.507433977957994	25.80505880178369	25.66325292853677	24.02425429172154
19	25.58328604084627	25.28148055087444	25.236715143270967	23.898518265008324
20	25.967188305508905	24.841840470067016	24.910144015064326	24.28082720935975
21	26.240471105373437	24.65028830366542	24.66934970303206	24.43989088792908
22	26.095488946554436	24.461118355405567	24.904584699761298	24.538807998278696
23	25.202817641741298	24.82461941908522	24.904042489898828	25.06852044927465
24	25.062671842147825	24.7909013192856	25.118267831149925	25.02815900741665
25	24.99682306799212	24.61530241504595	25.202168400501378	25.185706116460548
26	24.93725323698203	25.25120366920542	25.24947073213703	24.56207236167552
27	25.42850912472796	25.06271129409828	24.64737984487168	24.86139973630208
28	25.150944949319175	25.02877376814336	24.853459567259854	24.966821715277607
29	25.03418846617616	25.143649320923917	24.847180805360406	24.974981407539513
30	25.387344740790592	25.035127824847535	24.81533522703478	24.762192207327093
31	25.161240010051326	24.7863371295379	25.022745455963353	25.02967740444742
32	25.19531306409734	24.918914391504135	24.78807991011989	25.097692634278634
33	24.924293077130805	25.042564836462937	24.971515134886126	25.061626951520132
34	25.309286464177355	24.85844376957635	25.040253812017948	24.792015954228347
35	25.182750543702724	24.903606489196704	25.18722724607711	24.72641572102346
36	24.987074729724657	24.892626386036664	25.234460070301918	24.885838813936765
37	25.427385604491775	24.771324512683655	24.99992779428882	24.801362088535754
38	25.01112003281132	24.897175800497948	24.921293274257557	25.170410892433175
39	25.422438903225714	24.976930308592323	24.5828321060787	25.017798682103265
40	25.46474065067301	24.730715411064246	24.99527064202298	24.809273296239766
41	24.96747184383019	24.733817874095458	25.455574697787796	24.843135584286554
42	25.384085523380158	24.878229112664606	25.020036419351065	24.71764894460417
43	25.186104039197733	24.317064964208818	25.31144899052548	25.185382006067968
44	24.846099082876165	24.743137455938054	25.02198584530341	25.388777615882375
45	24.913753640831025	24.934692480646014	25.014837695110277	25.136716183412684
46	24.787758974484866	24.69476137596012	25.074549705339134	25.44292994421588
47	24.831476250714818	24.570242778172492	25.491419296330314	25.106861674782376
48	24.89537694624873	24.785483027815726	25.077907712213676	25.241232313721866
49	25.154586176919945	24.707217679864375	25.04223863311451	25.09595751010117
50	24.73765002000026	25.02126381425896	25.170579834250557	25.07050633149023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	5.0
2	9.0
3	21.5
4	34.0
5	46.0
6	58.0
7	59.0
8	60.0
9	74.0
10	88.0
11	135.5
12	183.0
13	372.0
14	561.0
15	731.0
16	901.0
17	872.5
18	844.0
19	878.0
20	912.0
21	1060.5
22	1209.0
23	1569.0
24	1929.0
25	2700.0
26	3471.0
27	4536.0
28	5601.0
29	7045.5
30	8490.0
31	10028.0
32	11566.0
33	13544.5
34	15523.0
35	18283.5
36	21044.0
37	23344.0
38	25644.0
39	28007.0
40	30370.0
41	33017.5
42	35665.0
43	37817.0
44	39969.0
45	43502.5
46	47036.0
47	49478.0
48	51920.0
49	53161.5
50	54403.0
51	55280.5
52	56158.0
53	55626.0
54	55094.0
55	52815.0
56	50536.0
57	48095.0
58	45654.0
59	41881.0
60	38108.0
61	33551.0
62	28994.0
63	24516.0
64	20038.0
65	17206.5
66	14375.0
67	12115.5
68	9856.0
69	8046.5
70	6237.0
71	4832.5
72	3428.0
73	2913.0
74	2398.0
75	1980.0
76	1562.0
77	1303.0
78	1044.0
79	875.0
80	706.0
81	551.5
82	397.0
83	316.5
84	236.0
85	169.5
86	103.0
87	70.0
88	37.0
89	29.0
90	21.0
91	13.5
92	6.0
93	5.5
94	5.0
95	6.5
96	8.0
97	6.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010108317845946348
2	1.444045406563764E-4
3	0.0010108317845946347
4	0.0
5	0.0
6	2.888090813127528E-4
7	0.0
8	0.0
9	0.0010108317845946347
10	0.0011552363252510113
11	0.0
12	1.444045406563764E-4
13	5.776181626255056E-4
14	1.444045406563764E-4
15	0.005054158922973174
16	8.664272439382584E-4
17	0.0
18	4.332136219691292E-4
19	0.0
20	2.888090813127528E-4
21	0.0
22	0.0
23	7.22022703281882E-4
24	4.332136219691292E-4
25	0.0010108317845946347
26	0.004765349841660421
27	0.005776181626255056
28	0.004043327138378539
29	0.0020216635691892694
30	0.004043327138378539
31	0.007653440654787949
32	0.003032495353783904
33	0.0037545180570657866
34	0.002599281731814775
35	0.0036101135164094103
36	0.008231058817413456
37	0.004765349841660421
38	0.007942249736100701
39	0.004620945301004045
40	0.0023104726505020225
41	0.003176899894440281
42	0.001444045406563764
43	0.001444045406563764
44	0.0011552363252510113
45	8.664272439382584E-4
46	0.0011552363252510113
47	0.0021660681098456458
48	0.0018772590285328933
49	7.22022703281882E-4
50	0.0011552363252510113
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	692499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.8319754381801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.93109807199128	30.101187372771903
2	16.745058220987968	16.018984270743843
3	7.832975965840442	11.240001420178054
4	4.2272263859542925	8.087863546583703
5	2.5074931766734245	5.996917601902372
6	1.5853576365778423	4.549847252011356
7	1.052392599419882	3.5236611876742012
8	0.7464386863171859	2.856290952802485
9	0.5163975686347068	2.2230284237344096
>10	1.8003057366283917	13.068393332002346
>50	0.03977220073980638	1.2664628841621586
>100	0.015483750234810903	1.0673617554331336
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	1.444045406563764E-4	0.0
20	0.0	0.0	0.0	2.888090813127528E-4	0.0
21	0.0	0.0	0.0	2.888090813127528E-4	0.0
22	0.0	0.0	0.0	2.888090813127528E-4	0.0
23	0.0	0.0	0.0	2.888090813127528E-4	0.0
24	0.0	0.0	0.0	2.888090813127528E-4	0.0
25	0.0	0.0	0.0	5.776181626255056E-4	0.0
26	0.0	0.0	0.0	7.22022703281882E-4	0.0
27	0.0	0.0	0.0	0.0010108317845946347	0.0
28	0.0	0.0	0.0	0.002166068109845646	0.0
29	0.0	0.0	0.0	0.005054158922973174	0.0
30	0.0	0.0	0.0	0.010974745089884606	0.0
31	0.0	0.0	0.0	0.026570435480773258	0.0
32	0.0	0.0	0.0	0.045487430306758565	0.0
33	0.0	0.0	0.0	0.06714811140521502	0.0
34	0.0	0.0	0.0	0.09068605153220438	0.0
35	0.0	0.0	0.0	0.1181229142569159	0.0
36	0.0	0.0	0.0	0.15841178110004492	0.0
37	0.0	0.0	0.0	0.2252710834239472	0.0
38	0.0	0.0	0.0	0.32519902555815966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	290	0.0	30.355188	1
GATTTCG	45	4.0676096E-5	29.332756	41
GTATCAA	715	0.0	27.701763	1
GCGATTC	40	7.029889E-4	27.499458	13
ATATGCT	85	2.3500797E-7	23.29366	4
TACGACC	50	0.0025796304	21.999567	4
TAAGGTT	50	0.0025796304	21.999567	4
TAGGACG	80	3.6124693E-6	21.999567	4
AATAGAG	95	6.8588815E-7	20.841696	5
TCTATAC	75	5.4691372E-5	20.532928	3
ATTTCGT	65	4.929893E-4	20.307291	42
CTAATAG	90	1.0011172E-5	19.555172	3
TTCGTAT	80	8.9778885E-5	19.25101	25
GTAGGAC	160	5.2750693E-11	19.24962	3
CGCTAGA	80	8.982868E-5	19.24962	37
GTACACT	165	8.185452E-11	18.666298	4
TGCGATT	60	0.0074098255	18.332972	12
TATCAAC	1105	0.0	18.316381	2
TCAACGC	1120	0.0	18.071074	4
ATCAACG	1130	0.0	17.911152	3
>>END_MODULE