FastQCFastQC Report
Thu 2 Feb 2017
SRR4063889_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063889_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467400
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15840.33889602053915274No Hit
TATCAACGCAGAGTACTTTTTTTTT10690.22871202396234488No Hit
CTATTGGAGCTGGAATTACCGCGGC5540.11852802738553701No Hit
GTCCTAAAGTGTGTATTTCTCATTT5350.1144629867351305No Hit
GTATCTGATCGTCTTCGAACCTCCG5210.11146769362430466No Hit
CTTTAGGACGTGAAATATGGCGAGG4950.10590500641848523No Hit
CTGTAGGACGTGGAATATGGCAAGA4810.10290971330765938No Hit
GAATAGGACCGCGGTTCTATTTTGT4790.10248181429182712No Hit
GTCCTACAGTGGACATTTCTAAATT4740.10141206675224647No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4700.10055626872058193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGA359.918565E-519.00053614
TGTCGAG250.006024922719.00053618
GCGTAAC250.006024922719.00053611
TCTTATA401.2853117E-518.9944342
AGATAGT350.002166736916.2861731
AACGGAC350.002166736916.28617315
GGGTAAT602.5600033E-514.250401511
GTATAGG400.005270806614.25040151
GCTCGTA400.005270806614.25040159
TATGTCG400.005270806614.250401516
TTGCGCG551.9550702E-413.8185718
TTTGCGC551.9550702E-413.8185717
ATTTGCG551.9550702E-413.8185716
TAGGACA852.687957E-713.4107074
ATAAGAC500.001497701313.2989513
ATCCCCG655.4351414E-513.15421712
CCGTCGT801.990249E-613.0628689
CGTCGTA801.990249E-613.06286810
GTCGTAG905.3760596E-712.66702411
GTCCTAC2700.012.6670231
Produced by FastQC (version 0.11.5)