##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063889_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467400
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.52124518613607	32.0	32.0	32.0	14.0	32.0
2	27.423095849379546	32.0	21.0	32.0	14.0	32.0
3	27.679328198545143	32.0	21.0	32.0	14.0	32.0
4	27.459738981600342	32.0	21.0	32.0	14.0	32.0
5	27.49783269148481	32.0	21.0	32.0	14.0	32.0
6	30.783403936670947	36.0	21.0	36.0	21.0	36.0
7	30.752282841249464	36.0	21.0	36.0	21.0	36.0
8	30.394197689345315	36.0	21.0	36.0	14.0	36.0
9	31.109067180145487	36.0	32.0	36.0	14.0	36.0
10	29.447488232777065	32.0	21.0	36.0	14.0	36.0
11	31.501014120667524	36.0	32.0	36.0	21.0	36.0
12	30.36280915703894	36.0	21.0	36.0	14.0	36.0
13	30.90241762943945	36.0	32.0	36.0	14.0	36.0
14	30.147693624304665	36.0	21.0	36.0	14.0	36.0
15	29.883893881044074	36.0	21.0	36.0	14.0	36.0
16	30.08188703465982	36.0	21.0	36.0	14.0	36.0
17	29.786416345742406	36.0	21.0	36.0	14.0	36.0
18	29.902441163885324	36.0	21.0	36.0	14.0	36.0
19	29.930246041934105	36.0	21.0	36.0	14.0	36.0
20	29.87025246041934	36.0	21.0	36.0	14.0	36.0
21	29.857948224219083	36.0	21.0	36.0	14.0	36.0
22	29.70995507060334	36.0	21.0	36.0	14.0	36.0
23	29.55703465982028	36.0	21.0	36.0	14.0	36.0
24	29.551444159178434	36.0	21.0	36.0	14.0	36.0
25	28.415265297389816	32.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	6.0
5	5.0
6	16.0
7	15.0
8	19.0
9	28.0
10	23.0
11	20.0
12	22.0
13	18.0
14	323.0
15	649.0
16	1017.0
17	1537.0
18	2484.0
19	3871.0
20	5973.0
21	8601.0
22	11064.0
23	14330.0
24	17837.0
25	21705.0
26	26024.0
27	30443.0
28	34614.0
29	37811.0
30	40566.0
31	43237.0
32	46375.0
33	44463.0
34	46870.0
35	27432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62148430644906	18.752220381713045	12.256721068693794	26.369574243144108
2	15.635751330811495	20.162649983739282	39.08584803929959	25.115750646149632
3	17.940286053552136	24.693888466928403	29.195220316862613	28.17060516265685
4	12.005391642953423	16.006333040929416	37.201480562271335	34.786794753845825
5	13.851522666147451	37.1443671087851	34.32476555028018	14.679344674787268
6	32.86894786843434	36.800212257893335	17.178981333287688	13.15185854038463
7	29.309909485802322	30.873044743543105	21.66499047782081	18.152055292833758
8	26.99944358842664	33.99268104776579	19.72693032015066	19.280945043656907
9	26.8927346896844	14.417829181951655	19.29397261329441	39.39546351506954
10	15.354007966490228	26.756448426500455	32.358462094960046	25.531081512049266
11	35.91213316581302	21.142876712035562	24.079904624716665	18.865085497434755
12	24.93262638305888	23.645089271380286	29.584884335714335	21.8374000098465
13	28.99478329369607	20.150571654254602	25.83008136520583	25.0245636868435
14	22.8051623343958	20.779915787761183	25.769287578159616	30.6456342996834
15	24.08681003722326	28.89629501199749	22.529597736209304	24.487297214569942
16	24.6873648744108	26.234807067416167	25.142029035144425	23.93579902302861
17	22.976614758923315	26.6955637609033	26.4594637983625	23.868357681810885
18	23.692706214810215	25.07696259981589	27.989766864336023	23.240564321037873
19	25.038962818339073	25.016056106458407	26.122000719313572	23.82298035588895
20	25.041157945892017	24.966656176474995	25.822570182593562	24.169615695039422
21	25.560236682337024	24.549370188643703	25.099331226826283	24.791061902192993
22	24.858169237817542	25.326799484485317	25.635722940831418	24.17930833686572
23	22.955533194887494	25.179729816523583	26.640690231004733	25.22404675758419
24	24.257805141203267	25.347228168724634	26.267318593618878	24.127648096453218
25	23.78339495481686	25.108167647543667	26.665653319745918	24.44278407789355
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	20.0
2	78.5
3	137.0
4	137.0
5	137.0
6	467.0
7	797.0
8	797.0
9	797.0
10	864.0
11	931.0
12	931.0
13	931.0
14	1129.0
15	1327.0
16	1327.0
17	1327.0
18	2385.5
19	3444.0
20	3444.0
21	3444.0
22	5924.0
23	8404.0
24	8404.0
25	8404.0
26	12938.0
27	17472.0
28	17472.0
29	17472.0
30	22114.5
31	26757.0
32	26757.0
33	26757.0
34	31496.5
35	36236.0
36	36236.0
37	36236.0
38	39768.0
39	43300.0
40	43300.0
41	43300.0
42	47371.0
43	51442.0
44	51442.0
45	51442.0
46	55779.5
47	60117.0
48	60117.0
49	60117.0
50	59802.0
51	59487.0
52	59487.0
53	59487.0
54	54123.5
55	48760.0
56	48760.0
57	48760.0
58	43993.5
59	39227.0
60	39227.0
61	39227.0
62	34315.0
63	29403.0
64	29403.0
65	29403.0
66	24215.0
67	19027.0
68	19027.0
69	19027.0
70	14222.5
71	9418.0
72	9418.0
73	9418.0
74	7312.5
75	5207.0
76	5207.0
77	5207.0
78	4498.5
79	3790.0
80	3790.0
81	3790.0
82	2623.0
83	1456.0
84	1456.0
85	1456.0
86	1094.0
87	732.0
88	732.0
89	732.0
90	495.5
91	259.0
92	259.0
93	259.0
94	155.5
95	52.0
96	52.0
97	52.0
98	125.0
99	198.0
100	198.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029525032092426188
2	0.0034231921266581087
3	0.0010697475395806588
4	0.0021394950791613176
5	0.0062045357295678226
6	0.009413778348309799
7	0.014976465554129226
8	0.025673940949935817
9	0.03551561831407788
10	0.04129225502781343
11	0.04129225502781343
12	0.04899443731279418
13	0.052845528455284556
14	0.052845528455284556
15	0.04685494223363286
16	0.0517757809157039
17	0.04813863928112966
18	0.06204535729567821
19	0.06161745827984596
20	0.06354300385109114
21	0.059050064184852376
22	0.06290115532734275
23	0.06632434745400086
24	0.058194266153187846
25	0.06439880188275567
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	467400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.11311618124877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.12057699709604	54.984510977951516
2	7.680444106033084	9.694735223753067
3	2.0745506473107707	3.927940680828286
4	0.8840528038260532	2.231813092729297
5	0.4975749016632689	1.5701751288773664
6	0.32199123206189034	1.2193122023079312
7	0.2142768142421727	0.9466574230549876
8	0.16243745757621167	0.8201547305755292
9	0.11524708458679421	0.6546242375168895
>10	0.7476335856407479	9.285486181580051
>50	0.10330912688148934	4.602378509658932
>100	0.07621165084062197	9.150885513343725
>500	0.001016155344541626	0.34417898604128855
>1k	6.774368963610841E-4	0.5671471117810799
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1584	0.33889602053915274	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1069	0.22871202396234488	No Hit
CTATTGGAGCTGGAATTACCGCGGC	554	0.11852802738553701	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	535	0.1144629867351305	No Hit
GTATCTGATCGTCTTCGAACCTCCG	521	0.11146769362430466	No Hit
CTTTAGGACGTGAAATATGGCGAGG	495	0.10590500641848523	No Hit
CTGTAGGACGTGGAATATGGCAAGA	481	0.10290971330765938	No Hit
GAATAGGACCGCGGTTCTATTTTGT	479	0.10248181429182712	No Hit
GTCCTACAGTGGACATTTCTAAATT	474	0.10141206675224647	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	470	0.10055626872058193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.139495079161318E-4
13	0.0	0.0	0.0	0.0	4.278990158322636E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGA	35	9.918565E-5	19.000536	14
TGTCGAG	25	0.0060249227	19.000536	18
GCGTAAC	25	0.0060249227	19.000536	11
TCTTATA	40	1.2853117E-5	18.994434	2
AGATAGT	35	0.0021667369	16.286173	1
AACGGAC	35	0.0021667369	16.286173	15
GGGTAAT	60	2.5600033E-5	14.2504015	11
GTATAGG	40	0.0052708066	14.2504015	1
GCTCGTA	40	0.0052708066	14.2504015	9
TATGTCG	40	0.0052708066	14.2504015	16
TTGCGCG	55	1.9550702E-4	13.81857	18
TTTGCGC	55	1.9550702E-4	13.81857	17
ATTTGCG	55	1.9550702E-4	13.81857	16
TAGGACA	85	2.687957E-7	13.410707	4
ATAAGAC	50	0.0014977013	13.298951	3
ATCCCCG	65	5.4351414E-5	13.154217	12
CCGTCGT	80	1.990249E-6	13.062868	9
CGTCGTA	80	1.990249E-6	13.062868	10
GTCGTAG	90	5.3760596E-7	12.667024	11
GTCCTAC	270	0.0	12.667023	1
>>END_MODULE