FastQCFastQC Report
Thu 2 Feb 2017
SRR4063889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467400
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6200.1326486949080017No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5840.12494651262302096No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5120.10954214805305948No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5110.10932819854514335No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG4880.10440735986307231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAC353.2174803E-431.4242152
GTACTAC752.1609594E-929.3324051
ATACCGT1850.028.5365856
ACCGTCG1700.028.4666428
GTATAGC554.953725E-627.9991151
GCATAAG407.0269336E-427.4991321
ATAGGGG407.03133E-427.496193
CGTCGTA1800.026.89091710
CCGTCGT1800.026.8880399
TACCGTC1900.026.6278887
GTATCAA6150.026.4707071
GGCGTAC450.001396318824.44628713
GCGTACT450.001396318824.44628714
GTCGTAG2000.024.20182411
TACGAAT1001.7389539E-924.19923441
TCAGATA2000.024.1966482
CGAATGC1053.0595402E-923.0468943
ATACGAA1053.0595402E-923.0468940
CTAGCGG1105.2350515E-921.99930629
CGGGTAA500.002578565421.99930444
Produced by FastQC (version 0.11.5)