##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063889_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467400
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.53125588361147	32.0	32.0	32.0	21.0	32.0
2	29.96609328198545	32.0	32.0	32.0	21.0	32.0
3	30.877567394094992	32.0	32.0	32.0	32.0	32.0
4	31.250021394950792	32.0	32.0	32.0	32.0	32.0
5	30.998151476251603	32.0	32.0	32.0	32.0	32.0
6	34.71662173727	36.0	36.0	36.0	32.0	36.0
7	34.760502781343604	36.0	36.0	36.0	32.0	36.0
8	34.6247432605905	36.0	36.0	36.0	32.0	36.0
9	34.9129567821994	36.0	36.0	36.0	32.0	36.0
10	34.52624304664099	36.0	36.0	36.0	32.0	36.0
11	34.97579589216945	36.0	36.0	36.0	36.0	36.0
12	34.76095421480531	36.0	36.0	36.0	32.0	36.0
13	34.894137783483096	36.0	36.0	36.0	32.0	36.0
14	34.78320068463842	36.0	36.0	36.0	32.0	36.0
15	34.68106974753958	36.0	36.0	36.0	32.0	36.0
16	34.74318142918271	36.0	36.0	36.0	32.0	36.0
17	34.68575096277279	36.0	36.0	36.0	32.0	36.0
18	34.7000106974754	36.0	36.0	36.0	32.0	36.0
19	34.713560119811724	36.0	36.0	36.0	32.0	36.0
20	34.71996576807874	36.0	36.0	36.0	32.0	36.0
21	34.732079589216944	36.0	36.0	36.0	32.0	36.0
22	34.695635430038514	36.0	36.0	36.0	32.0	36.0
23	34.63401797175867	36.0	36.0	36.0	32.0	36.0
24	34.632302096705175	36.0	36.0	36.0	32.0	36.0
25	34.57161103979461	36.0	36.0	36.0	32.0	36.0
26	34.492866923406076	36.0	36.0	36.0	32.0	36.0
27	34.480979888746255	36.0	36.0	36.0	32.0	36.0
28	34.46408001711596	36.0	36.0	36.0	32.0	36.0
29	34.424439452289256	36.0	36.0	36.0	32.0	36.0
30	34.40179075738126	36.0	36.0	36.0	32.0	36.0
31	34.40274497218656	36.0	36.0	36.0	32.0	36.0
32	34.34402866923406	36.0	36.0	36.0	32.0	36.0
33	34.32268934531451	36.0	36.0	36.0	32.0	36.0
34	34.3036649550706	36.0	36.0	36.0	32.0	36.0
35	34.28903294822422	36.0	36.0	36.0	32.0	36.0
36	34.22022250748823	36.0	36.0	36.0	32.0	36.0
37	34.210581942661534	36.0	36.0	36.0	32.0	36.0
38	34.171951219512195	36.0	36.0	36.0	32.0	36.0
39	34.149550706033374	36.0	36.0	36.0	32.0	36.0
40	34.15355584082157	36.0	36.0	36.0	32.0	36.0
41	34.124430894308944	36.0	36.0	36.0	32.0	36.0
42	34.040569105691056	36.0	36.0	36.0	32.0	36.0
43	34.043821138211385	36.0	36.0	36.0	32.0	36.0
44	33.96918271287976	36.0	36.0	36.0	32.0	36.0
45	33.912941805733844	36.0	36.0	36.0	32.0	36.0
46	33.8783055198973	36.0	36.0	36.0	32.0	36.0
47	33.893356867779204	36.0	36.0	36.0	32.0	36.0
48	33.824176294394526	36.0	36.0	36.0	32.0	36.0
49	33.80839965768079	36.0	36.0	36.0	32.0	36.0
50	32.989375267436884	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	13.0
22	29.0
23	97.0
24	258.0
25	589.0
26	1274.0
27	2634.0
28	4827.0
29	8430.0
30	14134.0
31	23283.0
32	38285.0
33	68904.0
34	129735.0
35	174906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32563657642662	19.61474850390365	13.300227008684526	25.75938791098521
2	16.164237605798938	20.857474176073392	37.52706484437179	25.451223373755873
3	18.391994848084828	24.939933547140665	29.68341823581137	26.984653368963134
4	12.51155327342747	17.031236628155753	37.72999572100984	32.72721437740693
5	15.435387248609327	36.05477107402653	33.139281129653405	15.37056054771074
6	33.73833975181857	36.952289259734705	16.619383825417202	12.689987163029526
7	29.645271715875054	30.924475823705606	21.278562259306806	18.15169020111254
8	27.16837826273	33.96191698759093	19.35066324347454	19.519041506204534
9	26.98161730096107	13.968027111913667	18.655059093359807	40.39529649376546
10	15.159424816912317	26.482726749522357	32.726214647442546	25.631633786122777
11	36.96640992725717	20.453359007274283	23.848951647411212	18.73127941805734
12	24.81685922122379	23.29589216944801	29.834617030380826	22.05263157894737
13	29.106457451679297	19.660974159067862	25.841146089628108	25.391422299624733
14	22.973519412750136	20.18896060967182	25.75465501637787	31.082864961200173
15	24.212424901151945	28.70851377026171	22.537356862879346	24.541704465707
16	24.86927573192753	25.957004338933153	25.19961659920068	23.97410332993864
17	23.121737270004278	26.875053487376977	26.11510483525888	23.888104407359865
18	23.834403080872914	25.303380402225073	27.880188275566965	22.982028241335044
19	25.16923406076166	25.263157894736842	26.174796747967477	23.39281129653402
20	25.49898480741294	24.80535901873988	25.6787027785682	24.016953395278982
21	26.016474112109545	24.619169875909286	25.09028669234061	24.274069319640564
22	25.05990586221652	25.489302524604195	25.64270432178006	23.80808729139923
23	23.284587982327583	25.40506423902695	26.472897656158068	24.837450122487404
24	24.43153129252283	25.582161593167253	25.952725312155007	24.03358180215492
25	24.29128315725063	25.39506283777712	26.181551325006314	24.13210267996594
26	23.56158052838327	26.116845105137486	26.577704177257438	23.743870189221802
27	24.489660558212183	25.696588465611864	25.474715705467656	24.339035270708305
28	23.756228751639426	25.622554232127843	25.93364413796645	24.687572878266284
29	24.276254559847665	25.214647139999357	25.766428823586047	24.74266947656693
30	23.83175470539529	25.365594825723324	26.46938631418369	24.33326415469769
31	24.973682859196877	25.061406661873953	25.401389887371195	24.563520591557975
32	24.300957018671813	25.72717800376132	25.07418873786075	24.89767623970611
33	23.537139299384453	25.62512168664646	26.20558036239621	24.632158651572887
34	24.409437026228463	25.388912542549846	26.269114000423627	23.932536430798066
35	25.059050569538467	25.43517702333781	25.612328731953205	23.89344367517052
36	23.809299658516128	25.69366585931549	25.895432332274932	24.60160214989345
37	24.81707003970971	25.432185403258934	25.06268827878954	24.688056278241817
38	24.199787751189618	25.56806511245764	25.6326811817466	24.599465954606142
39	25.06675396030707	25.076595890352344	24.813645194142765	25.043004955197823
40	25.41587058428346	25.395972982051507	25.673469649738013	23.514686783927015
41	24.435967162537736	25.27508969967458	26.264834946180194	24.02410819160749
42	25.199886176670066	26.15026819087231	25.341951385456717	23.307894247000903
43	24.202499796747098	25.622060856058432	25.891210488705557	24.28422885848891
44	24.009242717615724	25.62821596294355	25.371045903357974	24.991495416082756
45	24.312042413531938	25.913461332739978	25.242298854509432	24.532197399218653
46	24.245393627673323	25.408433105974375	25.478609145136033	24.867564121216272
47	25.047765147381956	25.252946676337373	25.507551493289345	24.191736682991326
48	25.266693767517488	25.977877147564133	24.599157020903313	24.156272064015063
49	24.64109816473327	25.712347934736563	25.20036457152149	24.446189329008682
50	24.607290637021443	26.09062161687998	24.78337334240491	24.51871440369367
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	5.5
2	7.0
3	13.0
4	19.0
5	25.0
6	31.0
7	42.0
8	53.0
9	76.5
10	100.0
11	119.5
12	139.0
13	254.5
14	370.0
15	464.5
16	559.0
17	654.0
18	749.0
19	787.5
20	826.0
21	1033.0
22	1240.0
23	1525.5
24	1811.0
25	2426.0
26	3041.0
27	4122.0
28	5203.0
29	6340.0
30	7477.0
31	8736.5
32	9996.0
33	12018.0
34	14040.0
35	16423.0
36	18806.0
37	21112.5
38	23419.0
39	24134.0
40	24849.0
41	26132.5
42	27416.0
43	27140.5
44	26865.0
45	28987.0
46	31109.0
47	32719.0
48	34329.0
49	34304.5
50	34280.0
51	32930.0
52	31580.0
53	30951.0
54	30322.0
55	30212.5
56	30103.0
57	28962.0
58	27821.0
59	25044.0
60	22267.0
61	19652.0
62	17037.0
63	14775.5
64	12514.0
65	10645.5
66	8777.0
67	7464.5
68	6152.0
69	5671.0
70	5190.0
71	4165.0
72	3140.0
73	2679.0
74	2218.0
75	1705.0
76	1192.0
77	1059.5
78	927.0
79	754.0
80	581.0
81	460.5
82	340.0
83	298.5
84	257.0
85	185.0
86	113.0
87	82.0
88	51.0
89	38.0
90	25.0
91	22.5
92	20.0
93	17.5
94	15.0
95	11.0
96	7.0
97	7.0
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0036371416345742404
2	8.557980316645272E-4
3	2.139495079161318E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	8.557980316645272E-4
10	2.139495079161318E-4
11	0.0
12	0.0
13	4.278990158322636E-4
14	2.139495079161318E-4
15	0.0034231921266581087
16	8.557980316645272E-4
17	0.0
18	0.0
19	0.0
20	2.139495079161318E-4
21	0.0
22	0.0
23	0.0010697475395806588
24	8.557980316645272E-4
25	0.0012836970474967909
26	0.0025673940949935818
27	0.003209242618741977
28	0.0023534445870774497
29	0.0010697475395806588
30	0.0023534445870774497
31	0.0051347881899871635
32	0.0023534445870774497
33	0.0023534445870774497
34	0.0014976465554129225
35	8.557980316645272E-4
36	0.00599058622165169
37	0.0017115960633290544
38	0.0051347881899871635
39	0.0025673940949935818
40	0.0014976465554129225
41	0.0014976465554129225
42	0.0019255455712451862
43	4.278990158322636E-4
44	0.0010697475395806588
45	4.278990158322636E-4
46	8.557980316645272E-4
47	0.0019255455712451862
48	0.0021394950791613176
49	4.278990158322636E-4
50	0.0012836970474967909
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	467400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.93298422744903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.59602098609537	61.071881172492546
2	7.028405938994589	9.68977991473646
3	1.7732845128534145	3.667133800659122
4	0.7847455634387549	2.1637941418833715
5	0.4323265207128812	1.4900778616704478
6	0.2751796728301956	1.1381373628151077
7	0.17125700951142717	0.8263679714843906
8	0.11476706274682597	0.6328988900926085
9	0.10005841271688781	0.6207592487073132
>10	0.5932755321655426	8.038322848876838
>50	0.07809613303979988	3.7490766220023626
>100	0.051338095015166514	6.434127510929613
>500	0.0012445598791555516	0.4776426536497965
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	620	0.1326486949080017	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	584	0.12494651262302096	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	512	0.10954214805305948	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	511	0.10932819854514335	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	488	0.10440735986307231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.139495079161318E-4	0.0
7	0.0	0.0	0.0	4.278990158322636E-4	0.0
8	0.0	0.0	0.0	8.557980316645272E-4	0.0
9	0.0	0.0	0.0	0.001069747539580659	0.0
10	0.0	0.0	0.0	0.001069747539580659	0.0
11	0.0	0.0	0.0	0.001069747539580659	0.0
12	0.0	0.0	0.0	0.001069747539580659	0.0
13	0.0	0.0	0.0	0.001069747539580659	2.139495079161318E-4
14	0.0	0.0	0.0	0.001069747539580659	2.139495079161318E-4
15	0.0	0.0	0.0	0.001069747539580659	2.139495079161318E-4
16	0.0	0.0	0.0	0.001069747539580659	2.139495079161318E-4
17	0.0	0.0	0.0	0.001069747539580659	2.139495079161318E-4
18	0.0	0.0	0.0	0.001069747539580659	2.139495079161318E-4
19	0.0	0.0	0.0	0.001069747539580659	2.139495079161318E-4
20	0.0	0.0	0.0	0.001069747539580659	6.418485237483953E-4
21	0.0	0.0	0.0	0.001069747539580659	6.418485237483953E-4
22	0.0	0.0	0.0	0.001069747539580659	6.418485237483953E-4
23	0.0	0.0	0.0	0.001069747539580659	8.557980316645272E-4
24	0.0	0.0	0.0	0.001069747539580659	8.557980316645272E-4
25	0.0	0.0	0.0	0.001069747539580659	8.557980316645272E-4
26	0.0	0.0	0.0	0.001069747539580659	8.557980316645272E-4
27	0.0	0.0	0.0	0.001069747539580659	8.557980316645272E-4
28	0.0	0.0	0.0	0.0012836970474967907	8.557980316645272E-4
29	0.0	0.0	0.0	0.002139495079161318	8.557980316645272E-4
30	0.0	0.0	0.0	0.0023534445870774497	8.557980316645272E-4
31	0.0	0.0	0.0	0.004920838682071031	8.557980316645272E-4
32	0.0	0.0	0.0	0.006632434745400086	8.557980316645272E-4
33	0.0	0.0	0.0	0.008985879332477536	8.557980316645272E-4
34	0.0	0.0	0.0	0.012836970474967908	8.557980316645272E-4
35	0.0	0.0	0.0	0.017543859649122806	8.557980316645272E-4
36	0.0	0.0	0.0	0.024390243902439025	8.557980316645272E-4
37	0.0	0.0	0.0	0.03423192126658109	8.557980316645272E-4
38	0.0	0.0	0.0	0.045785194694052206	8.557980316645272E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTAAC	35	3.2174803E-4	31.424215	2
GTACTAC	75	2.1609594E-9	29.332405	1
ATACCGT	185	0.0	28.536585	6
ACCGTCG	170	0.0	28.466642	8
GTATAGC	55	4.953725E-6	27.999115	1
GCATAAG	40	7.0269336E-4	27.499132	1
ATAGGGG	40	7.03133E-4	27.49619	3
CGTCGTA	180	0.0	26.890917	10
CCGTCGT	180	0.0	26.888039	9
TACCGTC	190	0.0	26.627888	7
GTATCAA	615	0.0	26.470707	1
GGCGTAC	45	0.0013963188	24.446287	13
GCGTACT	45	0.0013963188	24.446287	14
GTCGTAG	200	0.0	24.201824	11
TACGAAT	100	1.7389539E-9	24.199234	41
TCAGATA	200	0.0	24.196648	2
CGAATGC	105	3.0595402E-9	23.04689	43
ATACGAA	105	3.0595402E-9	23.04689	40
CTAGCGG	110	5.2350515E-9	21.999306	29
CGGGTAA	50	0.0025785654	21.999304	44
>>END_MODULE