FastQCFastQC Report
Thu 2 Feb 2017
SRR4063888_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063888_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091735
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74900.2422587964363052No Hit
TATCAACGCAGAGTACTTTTTTTTT52710.1704867978659232No Hit
GTACATGGAAGCAGTGGTATCAACG48350.15638468368084588No Hit
TCCATGTACTCTGCGTTGATACCAC45890.14842798622779768No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38870.1257222886178796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT2900.013.43205310
ACCGTCG3600.011.8755168
CGTCGTA3650.011.71321610
CGAGACT2050.011.582124
CCGTCGT3850.010.8576149
CCGACCA3350.010.77658759
TTTGCGC3000.010.76887717
TATACTG2850.010.6641965
TACCGTC4100.010.6588277
GTCTAGG2050.010.6565851
CGTCTTA3400.010.61965515
ATACCGT4450.010.4602836
GTCGTAG4150.010.3023211
TTGCGCG3350.010.21088718
ATTTGCG3300.010.07766216
GCATCGC3150.09.95361914
ATCGCCA3250.09.94034216
CGACCCG3350.09.9230745
AACTATC1151.0248852E-59.91491919
GCACCGT1151.027649E-59.9126726
Produced by FastQC (version 0.11.5)