##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063888_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3091735
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15653734877019	32.0	32.0	32.0	32.0	32.0
2	30.658396660774613	32.0	32.0	32.0	32.0	32.0
3	30.678782625289685	32.0	32.0	32.0	32.0	32.0
4	30.659283864884927	32.0	32.0	32.0	32.0	32.0
5	30.61594282821781	32.0	32.0	32.0	32.0	32.0
6	34.02952096476574	36.0	36.0	36.0	32.0	36.0
7	34.007817940412096	36.0	36.0	36.0	32.0	36.0
8	33.9503796412047	36.0	36.0	36.0	32.0	36.0
9	34.16879777859357	36.0	36.0	36.0	32.0	36.0
10	33.74471421386374	36.0	36.0	36.0	32.0	36.0
11	34.1772593705476	36.0	36.0	36.0	32.0	36.0
12	33.905525538249556	36.0	36.0	36.0	32.0	36.0
13	34.04986682235056	36.0	36.0	36.0	32.0	36.0
14	33.89505277780923	36.0	36.0	36.0	32.0	36.0
15	33.79610251201995	36.0	36.0	36.0	32.0	36.0
16	33.80941898319229	36.0	36.0	36.0	32.0	36.0
17	33.728866154440794	36.0	36.0	36.0	32.0	36.0
18	33.74152118470697	36.0	36.0	36.0	32.0	36.0
19	33.71887014896167	36.0	36.0	36.0	32.0	36.0
20	33.692997944519824	36.0	36.0	36.0	27.0	36.0
21	33.65090410400633	36.0	36.0	36.0	27.0	36.0
22	33.61556439992432	36.0	36.0	36.0	27.0	36.0
23	33.56383551630395	36.0	36.0	36.0	27.0	36.0
24	33.53461664728704	36.0	36.0	36.0	27.0	36.0
25	32.76427151744894	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	29.0
5	33.0
6	120.0
7	52.0
8	166.0
9	163.0
10	222.0
11	91.0
12	188.0
13	135.0
14	607.0
15	1301.0
16	2100.0
17	2660.0
18	3213.0
19	3980.0
20	5196.0
21	6590.0
22	8918.0
23	11930.0
24	17035.0
25	23159.0
26	33225.0
27	45994.0
28	65525.0
29	92197.0
30	132267.0
31	197116.0
32	303064.0
33	448316.0
34	831367.0
35	854769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.684580405393596	18.39886158985267	12.440295267042988	25.47626273771074
2	15.684237356335966	20.455183549683852	38.8152302311951	25.045348862785076
3	17.919805051669137	25.37876521295304	29.148740931742534	27.552688803635295
4	12.251005938595696	16.771551668370186	36.88372514264274	34.09371725039138
5	13.924622229697931	37.144294265395864	34.21396444115226	14.717119063753945
6	32.16036561242767	36.381095575702744	18.190887444073987	13.267651367795608
7	29.077090007033064	31.19920520563137	21.849307401797542	17.87439738553803
8	27.36303937139838	33.476155179392244	20.063368768637993	19.097436680571388
9	27.076077681677525	15.292322338125691	19.27483985119112	38.356760129005664
10	15.52333415745783	27.33228311394794	32.22716193528595	24.91722079330828
11	35.23938687602481	21.774949056810758	23.803939347752433	19.181724719412
12	24.72735769492412	24.096602335445343	29.578736974691466	21.597302994939067
13	28.781138386933495	20.588715033030024	25.87180363479137	24.75834294524511
14	23.218498833919448	20.543827864114558	26.187101071318303	30.050572230647692
15	24.419932628464007	28.099801005103835	23.181724184465583	24.298542181966575
16	24.737671991440315	26.487863102030722	24.81884140054126	23.9556235059877
17	23.12353800885706	26.608368252834012	26.28224794851979	23.98584578978914
18	23.725276123144624	25.636443496997707	27.462742027523788	23.175538352333877
19	24.75012526104142	25.804292407821467	26.18486587682082	23.260716454316295
20	24.949675394561417	25.1300315299554	26.19640498303416	23.723888092449027
21	25.62881682230649	25.079815847426794	25.183841854575917	24.1075254756908
22	24.753355593908076	25.556989099741443	25.469595412543516	24.22005989380696
23	23.386444691745016	25.433374830628242	26.155175282372582	25.025005195254156
24	23.898717411752063	25.99780419959774	25.954011187447684	24.149467201202512
25	24.018431646344403	25.455604183113667	26.034214941143606	24.491749229398323
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	289.0
1	289.0
2	883.5
3	1478.0
4	1478.0
5	1478.0
6	3789.0
7	6100.0
8	6100.0
9	6100.0
10	6283.0
11	6466.0
12	6466.0
13	6466.0
14	8367.0
15	10268.0
16	10268.0
17	10268.0
18	17831.5
19	25395.0
20	25395.0
21	25395.0
22	41076.0
23	56757.0
24	56757.0
25	56757.0
26	84333.5
27	111910.0
28	111910.0
29	111910.0
30	139949.5
31	167989.0
32	167989.0
33	167989.0
34	199674.5
35	231360.0
36	231360.0
37	231360.0
38	257092.0
39	282824.0
40	282824.0
41	282824.0
42	314023.0
43	345222.0
44	345222.0
45	345222.0
46	386115.5
47	427009.0
48	427009.0
49	427009.0
50	416569.0
51	406129.0
52	406129.0
53	406129.0
54	369067.0
55	332005.0
56	332005.0
57	332005.0
58	295956.5
59	259908.0
60	259908.0
61	259908.0
62	223386.0
63	186864.0
64	186864.0
65	186864.0
66	151017.0
67	115170.0
68	115170.0
69	115170.0
70	86417.0
71	57664.0
72	57664.0
73	57664.0
74	43628.5
75	29593.0
76	29593.0
77	29593.0
78	23846.0
79	18099.0
80	18099.0
81	18099.0
82	12360.5
83	6622.0
84	6622.0
85	6622.0
86	5084.0
87	3546.0
88	3546.0
89	3546.0
90	2476.0
91	1406.0
92	1406.0
93	1406.0
94	902.5
95	399.0
96	399.0
97	399.0
98	831.0
99	1263.0
100	1263.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03586982713589619
2	0.004754611892675149
3	0.0014231491379435818
4	0.0030727083660145515
5	0.007762631661510446
6	0.012096767672520447
7	0.02008580942415828
8	0.030338952077069995
9	0.04295322852702447
10	0.051330401861737825
11	0.04942208824494984
12	0.05786395017684245
13	0.05883427913453126
14	0.061357134424522154
15	0.05517937339390342
16	0.06148651161888066
17	0.05728175280222917
18	0.07316280340973597
19	0.07073698101551394
20	0.07413313236742476
21	0.06866694590577782
22	0.0733245249026841
23	0.07688239774754305
24	0.07118980119576872
25	0.07329218060409447
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3091735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.6445501882656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54991727384615	35.042527248965456
2	14.626800743256121	13.937746842116535
3	5.076853595564738	7.256532177970825
4	2.2222160102837787	4.235059289245314
5	1.2133450605755152	2.8904639817137148
6	0.7192773097745383	2.0561786330900187
7	0.4612335924078106	1.5382686929391578
8	0.3418841084913004	1.303113165246736
9	0.25279451600580416	1.0839852904641163
>10	1.3375210873493675	11.376143236179152
>50	0.1010723577987835	3.3492369629807937
>100	0.08050637550151964	7.988982679026399
>500	0.011597756191767051	3.764788588060329
>1k	0.004843768762218752	3.762188259093826
>5k	1.3644419048503524E-4	0.4147849529076531
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7490	0.2422587964363052	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5271	0.1704867978659232	No Hit
GTACATGGAAGCAGTGGTATCAACG	4835	0.15638468368084588	No Hit
TCCATGTACTCTGCGTTGATACCAC	4589	0.14842798622779768	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3887	0.1257222886178796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.468859717925372E-5	0.0
5	0.0	0.0	0.0	6.468859717925372E-5	0.0
6	0.0	0.0	0.0	6.468859717925372E-5	0.0
7	0.0	0.0	0.0	6.468859717925372E-5	0.0
8	0.0	0.0	0.0	6.468859717925372E-5	0.0
9	0.0	0.0	0.0	9.703289576888058E-5	0.0
10	0.0	0.0	0.0	1.2937719435850744E-4	0.0
11	0.0	0.0	0.0	1.2937719435850744E-4	0.0
12	0.0	0.0	0.0	1.2937719435850744E-4	6.468859717925372E-5
13	0.0	0.0	0.0	1.2937719435850744E-4	9.703289576888058E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCAT	290	0.0	13.432053	10
ACCGTCG	360	0.0	11.875516	8
CGTCGTA	365	0.0	11.713216	10
CGAGACT	205	0.0	11.58212	4
CCGTCGT	385	0.0	10.857614	9
CCGACCA	335	0.0	10.7765875	9
TTTGCGC	300	0.0	10.768877	17
TATACTG	285	0.0	10.664196	5
TACCGTC	410	0.0	10.658827	7
GTCTAGG	205	0.0	10.656585	1
CGTCTTA	340	0.0	10.619655	15
ATACCGT	445	0.0	10.460283	6
GTCGTAG	415	0.0	10.30232	11
TTGCGCG	335	0.0	10.210887	18
ATTTGCG	330	0.0	10.077662	16
GCATCGC	315	0.0	9.953619	14
ATCGCCA	325	0.0	9.940342	16
CGACCCG	335	0.0	9.923074	5
AACTATC	115	1.0248852E-5	9.914919	19
GCACCGT	115	1.027649E-5	9.912672	6
>>END_MODULE