FastQCFastQC Report
Thu 2 Feb 2017
SRR4063888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091735
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC4800.018.791551
TAACGGC3800.017.36999736
TAGGACG14100.016.8504144
AGGACGT14800.016.0534345
CGTCGTA5650.015.96501810
TTAGGAC10400.015.6527373
GTAGGAC15250.015.5792233
TAGGACC15750.015.5041644
ATACCGT6400.015.4681546
ACCGTCG6300.015.3644878
AACGAAT2800.014.92968731
GGACGTG15650.014.9003846
TTAACGG4600.014.82743435
GTATTAG9500.014.820961
GTATCAA91150.014.6746211
CCGTCGT6300.014.6670499
GTATAGA4350.014.6665751
GTCCTAC13850.014.61362651
ATTTCGT5750.014.53833442
TGTAGGA16750.014.4465032
Produced by FastQC (version 0.11.5)