##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063888_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3091735
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.072393655989273	32.0	32.0	32.0	32.0	32.0
2	30.879063050358457	32.0	32.0	32.0	32.0	32.0
3	31.19834235469728	32.0	32.0	32.0	32.0	32.0
4	31.385346092080983	32.0	32.0	32.0	32.0	32.0
5	31.22330827189264	32.0	32.0	32.0	32.0	32.0
6	34.84675853525609	36.0	36.0	36.0	32.0	36.0
7	34.885666138915525	36.0	36.0	36.0	32.0	36.0
8	34.805549634752005	36.0	36.0	36.0	32.0	36.0
9	35.00986339385491	36.0	36.0	36.0	36.0	36.0
10	34.735305580847	36.0	36.0	36.0	32.0	36.0
11	35.021583027005875	36.0	36.0	36.0	36.0	36.0
12	34.88661641440809	36.0	36.0	36.0	32.0	36.0
13	34.956455841137746	36.0	36.0	36.0	36.0	36.0
14	34.888395674273504	36.0	36.0	36.0	32.0	36.0
15	34.817690714113596	36.0	36.0	36.0	32.0	36.0
16	34.85009452621263	36.0	36.0	36.0	32.0	36.0
17	34.80827043714937	36.0	36.0	36.0	32.0	36.0
18	34.80880153053221	36.0	36.0	36.0	32.0	36.0
19	34.80689515757334	36.0	36.0	36.0	32.0	36.0
20	34.800307270836605	36.0	36.0	36.0	32.0	36.0
21	34.80129506571553	36.0	36.0	36.0	32.0	36.0
22	34.77245074367629	36.0	36.0	36.0	32.0	36.0
23	34.71645273608508	36.0	36.0	36.0	32.0	36.0
24	34.687329282749005	36.0	36.0	36.0	32.0	36.0
25	34.65841509702481	36.0	36.0	36.0	32.0	36.0
26	34.58850418939527	36.0	36.0	36.0	32.0	36.0
27	34.5684853326692	36.0	36.0	36.0	32.0	36.0
28	34.54256364145051	36.0	36.0	36.0	32.0	36.0
29	34.51032801970415	36.0	36.0	36.0	32.0	36.0
30	34.490946992546256	36.0	36.0	36.0	32.0	36.0
31	34.4807866780303	36.0	36.0	36.0	32.0	36.0
32	34.431127506076685	36.0	36.0	36.0	32.0	36.0
33	34.40729881441973	36.0	36.0	36.0	32.0	36.0
34	34.384061053098016	36.0	36.0	36.0	32.0	36.0
35	34.34620463914275	36.0	36.0	36.0	32.0	36.0
36	34.301282289717584	36.0	36.0	36.0	32.0	36.0
37	34.28112273529264	36.0	36.0	36.0	32.0	36.0
38	34.23724413638297	36.0	36.0	36.0	32.0	36.0
39	34.209676120366076	36.0	36.0	36.0	32.0	36.0
40	34.19158757137983	36.0	36.0	36.0	32.0	36.0
41	34.17018437867411	36.0	36.0	36.0	32.0	36.0
42	34.08927091099334	36.0	36.0	36.0	32.0	36.0
43	34.0880059254755	36.0	36.0	36.0	32.0	36.0
44	34.02349813292536	36.0	36.0	36.0	32.0	36.0
45	33.95737571298963	36.0	36.0	36.0	32.0	36.0
46	33.95333364599489	36.0	36.0	36.0	32.0	36.0
47	33.909779136957084	36.0	36.0	36.0	32.0	36.0
48	33.86308140898233	36.0	36.0	36.0	32.0	36.0
49	33.848942745739855	36.0	36.0	36.0	32.0	36.0
50	33.1214745118841	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	1.0
19	11.0
20	24.0
21	78.0
22	274.0
23	798.0
24	2276.0
25	5427.0
26	11325.0
27	21642.0
28	37446.0
29	60319.0
30	91205.0
31	139914.0
32	215419.0
33	363208.0
34	717362.0
35	1425003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.359894784101684	19.55677220602753	13.26995408125638	25.813378928614405
2	15.137790347527563	21.398047563076418	37.828837354927316	25.635324734468707
3	17.42439262949631	25.947390602475185	29.616631988120563	27.011584779907942
4	12.1380713418194	17.66978735240892	37.14729755299209	33.04484375277959
5	14.086039068678266	37.284566756206466	33.54569521643996	15.083698958675306
6	32.46750684406908	36.897359664239545	17.586346535012133	13.048786956679242
7	29.042343228751243	31.391510395137484	21.700120385518286	17.86602599059298
8	27.43084384657805	33.68377302711908	19.728275547548545	19.157107578754324
9	27.089645241515292	15.029454583209482	18.998327462254693	38.88257271302054
10	15.413835028785105	27.512345137938542	32.21415511127686	24.859664721999494
11	35.60605006965349	21.661475942456867	23.615363939900373	19.117110047989268
12	24.682952894056502	24.09965543596423	29.52007822150231	21.697313448476955
13	29.03566779063468	20.49920351907107	25.78683421067527	24.678294479618984
14	23.329470338441695	20.36724434774199	26.045361673031497	30.257923640784817
15	24.59889986550749	28.121581486999382	23.02713591765099	24.252382729842132
16	24.879508544292435	26.4306874538484	24.77031341524909	23.919490586610078
17	23.3260612568671	26.620683855505078	26.084803516472142	23.968451371155677
18	23.874608470872563	25.599277296421025	27.354123459920743	23.17199077278567
19	24.83586077073229	25.761166464784335	26.176467258675146	23.22650550580823
20	25.157630805914383	25.026118164722654	26.157236850191385	23.65901417917158
21	25.765144813510858	25.005603649730652	25.21179855323952	24.017452983518965
22	24.903363196862855	25.467707248787168	25.45716299380509	24.171766560544885
23	23.598642307378814	25.239543347448475	26.139302575010127	25.02251177016259
24	24.191309320424903	25.91122240457272	25.786340704189183	24.111127570813196
25	24.35198620296163	25.20213942072881	26.034308876927025	24.411565499382537
26	23.851830527607916	25.807584758029435	26.419501907591386	23.921082806771263
27	24.56393501163972	25.91273233752829	25.470141536546652	24.053191114285337
28	23.860170622245583	25.376137144705453	26.206186067197674	24.557506165851294
29	24.18015129696979	25.415309793936476	26.11434992373049	24.29018898536324
30	24.241049075635036	25.456295631180385	26.300476511465128	24.00217878171945
31	24.506211275555508	25.17749158594946	25.748466386656553	24.567830751838475
32	23.983615189847214	25.72728296013383	25.604791496045472	24.684310353973483
33	23.64354023497112	25.61988657057446	26.12428330315492	24.612289891299497
34	24.148210058913218	25.510744045443463	26.424587908266933	23.916457987376386
35	24.79830382109259	25.510511360420228	25.883580153671737	23.807604664815447
36	24.065793980907184	25.804286487365964	25.834433382316647	24.295486149410213
37	24.785760539572276	25.56605988936595	25.486328588700992	24.161850982360782
38	24.14485092690141	25.708053955039194	25.795971388988715	24.351123729070686
39	24.554463964280135	25.459169695560213	25.487601455939018	24.49876488422063
40	24.938787286386226	25.482893108532	25.786546981930204	23.79177262315157
41	24.008825035361273	25.607740051538673	26.169347913207762	24.214086999892288
42	24.90410562790558	26.083657347794066	25.631411758579386	23.38082526572097
43	24.188190133398972	25.43763097554972	25.860860503094894	24.513318387956414
44	23.98029302354015	25.65338712040669	25.814269638971737	24.55205021708143
45	24.197843449730037	25.82041513123699	25.69009835051753	24.291643068515448
46	24.06123987656026	25.512216374687025	25.83873460359005	24.587809145162662
47	24.545498358014065	25.42010290188224	25.933480243608415	24.100918496495275
48	24.638360869001293	26.23506195808308	25.09054118350273	24.0360359894129
49	24.148841132752384	25.799213772501183	25.742319985664963	24.309625109081466
50	24.045917943234265	26.33731918055147	25.149683554846558	24.467079321367706
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	51.5
2	53.0
3	203.5
4	354.0
5	393.0
6	432.0
7	454.0
8	476.0
9	664.5
10	853.0
11	1245.5
12	1638.0
13	3072.5
14	4507.0
15	6015.0
16	7523.0
17	8304.0
18	9085.0
19	9497.5
20	9910.0
21	11268.0
22	12626.0
23	14618.0
24	16610.0
25	20403.0
26	24196.0
27	30525.0
28	36854.0
29	44445.5
30	52037.0
31	59031.5
32	66026.0
33	77300.0
34	88574.0
35	101282.0
36	113990.0
37	127573.5
38	141157.0
39	149110.0
40	157063.0
41	167564.5
42	178066.0
43	180470.0
44	182874.0
45	197279.0
46	211684.0
47	223923.5
48	236163.0
49	236235.0
50	236307.0
51	226381.0
52	216455.0
53	209804.5
54	203154.0
55	199459.0
56	195764.0
57	188219.5
58	180675.0
59	162831.0
60	144987.0
61	128059.5
62	111132.0
63	95428.5
64	79725.0
65	66935.5
66	54146.0
67	45969.5
68	37793.0
69	33877.5
70	29962.0
71	23426.5
72	16891.0
73	14408.5
74	11926.0
75	9266.5
76	6607.0
77	5832.5
78	5058.0
79	4227.5
80	3397.0
81	2749.5
82	2102.0
83	1737.0
84	1372.0
85	1008.5
86	645.0
87	472.5
88	300.0
89	239.0
90	178.0
91	144.0
92	110.0
93	86.0
94	62.0
95	71.5
96	81.0
97	71.0
98	61.0
99	52.5
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0060483838362602235
2	2.910986873066417E-4
3	1.9406579153776116E-4
4	0.0
5	0.0
6	2.2641009012738802E-4
7	3.234429858962686E-5
8	0.0
9	8.409517633302983E-4
10	0.0010350175548680595
11	6.468859717925372E-5
12	6.468859717925372E-5
13	3.234429858962686E-4
14	1.9406579153776116E-4
15	0.003654905740627835
16	7.115745689717909E-4
17	0.0
18	3.5578728448589544E-4
19	0.0
20	5.498530760236565E-4
21	0.0
22	1.2937719435850744E-4
23	6.145416732029103E-4
24	2.910986873066417E-4
25	0.0018436250196087309
26	0.004528201802547761
27	0.005369153565878059
28	0.0035578728448589546
29	0.0021670680055049995
30	0.0032020855603730595
31	0.005821973746132835
32	0.0029433311716560444
33	0.00391366012934485
34	0.0022317566026842533
35	0.002587543887170149
36	0.006662925509463133
37	0.003266774157552313
38	0.006404171120746119
39	0.003946004427934477
40	0.0018436250196087309
41	0.0024581666928116413
42	0.0016819035266605966
43	0.0010350175548680595
44	0.0012290833464058206
45	0.0012290833464058206
46	7.762631661510447E-4
47	0.0016819035266605966
48	0.0015525263323020893
49	2.910986873066417E-4
50	0.0014878377351228355
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3091735.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.02048969677392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.13620678108863	40.36778967188159
2	13.481570665685066	14.295989571525695
3	4.47905659467811	7.12445322088194
4	2.0024581055101605	4.246852374056916
5	1.0537301334752784	2.7934643842553113
6	0.6414140530706439	2.0404852315318847
7	0.4183843233086833	1.5528059192295842
8	0.2955600548206354	1.253659107311635
9	0.21961664235246098	1.0479763730779867
>10	1.1224784066411664	10.554488953531264
>50	0.07517276269487741	2.7606732597796833
>100	0.06366591418500034	6.956282787233647
>500	0.008167700063978772	2.976328291429633
>1k	0.002517862425459601	2.028750854273183
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.234429858962686E-5	0.0
4	0.0	0.0	0.0	3.234429858962686E-5	0.0
5	0.0	0.0	0.0	3.234429858962686E-5	0.0
6	0.0	0.0	0.0	3.234429858962686E-5	0.0
7	0.0	0.0	0.0	3.234429858962686E-5	0.0
8	0.0	0.0	0.0	3.234429858962686E-5	0.0
9	0.0	0.0	0.0	6.468859717925372E-5	0.0
10	0.0	0.0	0.0	9.703289576888058E-5	0.0
11	0.0	0.0	0.0	9.703289576888058E-5	0.0
12	0.0	0.0	0.0	9.703289576888058E-5	1.617214929481343E-4
13	0.0	0.0	0.0	9.703289576888058E-5	2.2641009012738802E-4
14	0.0	0.0	0.0	9.703289576888058E-5	2.2641009012738802E-4
15	0.0	3.234429858962686E-5	0.0	1.2937719435850744E-4	2.587543887170149E-4
16	0.0	3.234429858962686E-5	0.0	1.2937719435850744E-4	2.910986873066417E-4
17	0.0	3.234429858962686E-5	0.0	1.2937719435850744E-4	3.234429858962686E-4
18	0.0	3.234429858962686E-5	0.0	1.2937719435850744E-4	3.5578728448589544E-4
19	0.0	3.234429858962686E-5	0.0	1.617214929481343E-4	3.5578728448589544E-4
20	0.0	3.234429858962686E-5	0.0	2.910986873066417E-4	3.5578728448589544E-4
21	0.0	3.234429858962686E-5	0.0	4.2047588166514916E-4	3.5578728448589544E-4
22	0.0	3.234429858962686E-5	0.0	6.468859717925372E-4	3.8813158307552233E-4
23	0.0	3.234429858962686E-5	0.0	0.0013261162421747013	3.8813158307552233E-4
24	0.0	3.234429858962686E-5	0.0	0.0023287894984531337	3.8813158307552233E-4
25	0.0	3.234429858962686E-5	0.0	0.0026198881857597755	3.8813158307552233E-4
26	0.0	3.234429858962686E-5	0.0	0.0031373969631938053	4.851644788444029E-4
27	0.0	3.234429858962686E-5	0.0	0.004107725920882611	5.821973746132834E-4
28	0.0	6.468859717925372E-5	0.0	0.005821973746132835	5.821973746132834E-4
29	0.0	9.703289576888058E-5	0.0	0.008409517633302983	5.821973746132834E-4
30	0.0	9.703289576888058E-5	0.0	0.015169476038534998	5.821973746132834E-4
31	0.0	9.703289576888058E-5	0.0	0.029400967417970814	5.821973746132834E-4
32	0.0	9.703289576888058E-5	0.0	0.04748143032957223	5.821973746132834E-4
33	0.0	9.703289576888058E-5	0.0	0.06410639980464043	5.821973746132834E-4
34	0.0	1.2937719435850744E-4	0.0	0.0852595710822564	7.115745689717909E-4
35	0.0	1.2937719435850744E-4	0.0	0.10883856475409438	7.115745689717909E-4
36	0.0	1.2937719435850744E-4	0.0	0.1424442909887167	7.115745689717909E-4
37	0.0	1.2937719435850744E-4	0.0	0.1928043638927657	7.115745689717909E-4
38	0.0	1.2937719435850744E-4	0.0	0.26363837780404853	7.115745689717909E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	480	0.0	18.79155	1
TAACGGC	380	0.0	17.369997	36
TAGGACG	1410	0.0	16.850414	4
AGGACGT	1480	0.0	16.053434	5
CGTCGTA	565	0.0	15.965018	10
TTAGGAC	1040	0.0	15.652737	3
GTAGGAC	1525	0.0	15.579223	3
TAGGACC	1575	0.0	15.504164	4
ATACCGT	640	0.0	15.468154	6
ACCGTCG	630	0.0	15.364487	8
AACGAAT	280	0.0	14.929687	31
GGACGTG	1565	0.0	14.900384	6
TTAACGG	460	0.0	14.827434	35
GTATTAG	950	0.0	14.82096	1
GTATCAA	9115	0.0	14.674621	1
CCGTCGT	630	0.0	14.667049	9
GTATAGA	435	0.0	14.666575	1
GTCCTAC	1385	0.0	14.6136265	1
ATTTCGT	575	0.0	14.538334	42
TGTAGGA	1675	0.0	14.446503	2
>>END_MODULE